Rabbit Recombinant Monoclonal PIEZO2 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-PIEZO2 antibody [EPR27152-48] - BSA and Azide free (AB318188), expandable thumbnail](https://content.abcam.com/products/images/piezo2-antibody-epr27152-48-bsa-and-azide-free-ab318188--western-blot-img432793.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PIEZO2 antibody [EPR27152-48] - BSA and Azide free (AB318188)")
![Western blot - Anti-PIEZO2 antibody [EPR27152-48] - BSA and Azide free (AB318188), expandable thumbnail](https://content.abcam.com/products/images/piezo2-antibody-epr27152-48-bsa-and-azide-free-ab318188--western-blot-img432792.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PIEZO2 antibody [EPR27152-48] - BSA and Azide free (AB318188)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | Flow Cyt | IHC-P | IHC-Fr | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Pore-forming subunit of the mechanosensitive non-specific cation Piezo channel required for rapidly adapting mechanically activated (MA) currents and has a key role in sensing touch and tactile pain. Piezo channels are homotrimeric three-blade propeller-shaped structure that utilize a cap-motion and plug-and-latch mechanism to gate their ion-conducting pathways. In inner ear hair cells, PIEZO1/2 subunits may constitute part of the mechanotransducer (MET) non-selective cation channel complex where they may act as pore-forming ion-conducting component in the complex. Required for Merkel-cell mechanotransduction. Plays a major role in light-touch mechanosensation.
Alternative names
C18orf30, C18orf58, FAM38B, Piezo-type mechanosensitive ion channel component 2, Protein FAM38B
Recommended products
Rabbit Recombinant Monoclonal PIEZO2 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27152-48
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab318188 is the carrier-free version of Anti-PIEZO2 antibody [EPR27152-48] ab318187.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PIEZO2 also known as FAM38B is a mechanosensitive ion channel protein. It has a high molecular mass of approximately 287 kDa. This protein exists in mechanosensitive tissues such as the skin bladder and lungs. PIEZO2 detects mechanical stimuli by opening its ion-conducting pore allowing the influx of cations into the cell which sends a signal.
- Biological function summary
PIEZO2 plays an important role in the sensory pathways of touch and proprioception. It is not a part of a larger protein complex but operates as a homotrimer. The channel's sensitivity to mechanical forces enables cells to transduce physical touch into electrical signals facilitating an organism's ability to perceive its environment.
- Pathways
Sensory transduction and mechanotransduction heavily involve PIEZO2. This protein interacts within pathways that transmit information about mechanical loads to the nervous system. Although not directly part of larger pathways' cascades its role is well observed in touch-related signaling paths where it occasionally interacts with proteins like TRPV1.
- Associated diseases and disorders
Mutations in PIEZO2 relate to Gordon Syndrome and Marden-Walker Syndrome. Dysfunction in PIEZO2 can lead to defects in touch and balance implicating disorders with sensory processing. There is also a notable connection to the protein TRPA1 which participates in similar sensory pathways and can correlate with sensory-related dysfunctions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-PIEZO2 antibody [EPR27152-48] - BSA and Azide free (ab318188), expandable thumbnail](https://content.abcam.com/products/images/piezo2-antibody-epr27152-48-bsa-and-azide-free-ab318188--western-blot-img432793.jpg "Western blot - Anti-PIEZO2 antibody [EPR27152-48] - BSA and Azide free (ab318188)")
Western blot - Anti-PIEZO2 antibody [EPR27152-48] - BSA and Azide free (ab318188)
This data was developed using Anti-PIEZO2 antibody [EPR27152-48] ab318187, the same antibody clone in a different buffer formulation.
Negative control: T-47D, MCF7.
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-PIEZO2 antibody [EPR27152-48] (Anti-PIEZO2 antibody [EPR27152-48] ab318187) at 1/1000 dilution
Lane 1: MKL-1 (human Skin Loosely packed floati aggregates with irregular outline and no central necrosis) whole cell lysate at 48 µg with NFDM/TBST
Lane 2: T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 48 µg with NFDM/TBST
Lane 3: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 48 µg with NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 320 kDa, 124 kDa
Exposure time: 92s
![Western blot - Anti-PIEZO2 antibody [EPR27152-48] - BSA and Azide free (ab318188), expandable thumbnail](https://content.abcam.com/products/images/piezo2-antibody-epr27152-48-bsa-and-azide-free-ab318188--western-blot-img432792.jpg "Western blot - Anti-PIEZO2 antibody [EPR27152-48] - BSA and Azide free (ab318188)")
Western blot - Anti-PIEZO2 antibody [EPR27152-48] - BSA and Azide free (ab318188)
This data was developed using Anti-PIEZO2 antibody [EPR27152-48] ab318187, the same antibody clone in a different buffer formulation.
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-PIEZO2 antibody [EPR27152-48] (Anti-PIEZO2 antibody [EPR27152-48] ab318187) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg with NFDM/TBST
Lane 2: HeLa transfected with siRNA specifically targeting PIEZO2 whole cell lysate at 20 µg with NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 320 kDa, 124 kDa
Exposure time: 92s
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