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AB192369

Anti-PIF1 antibody

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(2 Publications)

Rabbit Polyclonal PIF1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PIF1.

View Alternative Names

C15orf20, PIF1, ATP-dependent DNA helicase PIF1, DNA repair and recombination helicase PIF1, PIF1/RRM3 DNA helicase-like protein

1 Images
Western blot - Anti-PIF1 antibody (AB192369)
  • WB

Supplier Data

Western blot - Anti-PIF1 antibody (AB192369)

All lanes:

Western blot - Anti-PIF1 antibody (ab192369)

Lane 1:

HeLa whole cell lysate

Lane 2:

Raw264.7 whole cell lysate

Lane 3:

PC12 whole cell lysate

Predicted band size: 70 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human PIF1. The exact immunogen used to generate this antibody is proprietary information.

Q9H611

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab192369 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage buffer
Preservative: 0.1% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PIF1 also known as Pif1p is a helicase enzyme that plays a critical role in DNA replication and repair. It is part of the PIF1 family of helicases which are conserved from bacteria to humans. The enzyme has a molecular mass of approximately 70 kDa. PIF1 locates itself in the mitochondria and the nucleus where it acts on both nuclear and mitochondrial DNA. The enzyme functions by unwinding DNA helices facilitating various genetic processes such as replication recombination and repair.
Biological function summary

The protein maintains genome stability by resolving R-loops and DNA structures that can hinder replication. PIF1 interacts with proteins involved in DNA maintenance but it is not typically known to be part of a larger protein complex. The helicase is noted for suppressing the instability of genomic regions with G-quadruplex structures being particularly active at telomeres. Its activity prevents the accumulation of deleterious DNA structures that might otherwise disrupt genomic integrity.

Pathways

PIF1 engages in DNA replication and repair pathways. Within these biological pathways PIF1 ensures correct fork progression and prevents stalling which is linked to several other proteins such as Replication Protein A (RPA) and DNA pol Δ. PIF1 also has a role in the maintenance of telomeres interacting with telomeric proteins to prevent aberrant telomere elongation and reduce genomic instability.

PIF1's function relates closely to certain cancer types and mitochondrial disorders. Deficiencies or mutations in the PIF1 helicase can result in genomic instability contributing to tumor progression. PIF1 has been linked with BRCA1 in the context of breast cancer where it may play a complementary or compensatory role. Additionally defects in mitochondrial PIF1 functionality can contribute to mitochondrial myopathies disorders that involve proteins like mitochondrial DNA polymerase γ.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA/telomeric DNA hybrid that is the intermediate in the telomerase reaction. Possesses an intrinsic strand annealing activity.
See full target information PIF1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of applied toxicology : JAT 43:1522-1532 PubMed37183367

2023

Petite Integration Factor 1 knockdown enhances gemcitabine sensitivity in pancreatic cancer cells via increasing DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Wang,Xiangdong Hua,Xibo Fu,Zhiqiang Hao,Ao Jiao,Siyuan Li

Cancer management and research 12:7827-7835 PubMed32943924

2020

PIF1 Affects the Proliferation and Apoptosis of Cervical Cancer Cells by Influencing TERT.

Applications

Unspecified application

Species

Unspecified reactive species

Jiancai Wang,Xiaoyan Zhu,Pian Ying,Yingping Zhu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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