AB64798

Anti-PIG3 antibody

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(4 Publications)

Rabbit Polyclonal PIG3 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

PIG3, TP53I3, Quinone oxidoreductase PIG3, NADPH:quinone reductase PIG3, Tumor protein p53-inducible protein 3, p53-induced gene 3 protein, Protein PIG3

1 Images

Project

Western blot - Anti-PIG3 antibody (AB64798)

All lanes:

Western blot - Anti-PIG3 antibody (ab64798) at 1 µg/mL

Lane 1:

Caco-2 whole cell lysate (<a href='/en-us/products/unavailable/caco-2-whole-cell-lysate-ab3950'>ab3950</a>) at 10 µg

Lane 2:

A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg

Lane 3:

WI38 (Human lung fibroblast cell line) Whole Cell Lysate at 10 µg

Lane 4:

SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate at 10 µg

Lane 5:

TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate at 10 µg

Lane 6:

Lung (Human) Tissue Lysate at 10 µg

Lane 7:

Human skeletal muscle tissue lysate - total protein (<a href='/en-us/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 10 µg

Lane 8:

Human colon tissue lysate - total protein (<a href='/en-us/products/unavailable/human-colon-tissue-lysate-total-protein-ab30051'>ab30051</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 36 kDa

Observed band size: 35 kDa

false

Exposure time: 2min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PIG3 also known as PIG00 or P@IG3 str0h is a protein linked to cellular stress response with a molecular mass of approximately 55 kDa. It shows expression mainly in tissues that undergo oxidative stress or DNA damage acting as an important player in apoptosis. PIG3 is mainly known as p53-induced gene 3 aligning with its dependency on p53 for activation. High expression levels become evident in tissues encountering high metabolic or environmental stress.

Biological function summary

PIG3 mediates cell apoptosis in response to DNA damage and oxidative stress. It forms part of a complex with p53 contributing to the regulation of apoptotic pathways. As an oxidoreductase PIG3 initiates pro-apoptotic changes within the cell. Its enzymatic activity involves producing reactive oxygen species (ROS) further amplifying the cell’s apoptotic initiations. Its role connects to cellular response mechanisms in stress conditions dealing with damaged or unhealthy cells.

Pathways

PIG3 operates within the important p53 apoptotic pathway and the DNA damage signaling pathway. It significantly interacts with p53 as PIG3 expression depends on p53 transcription factor activation. Also PIG3 connects with caspase pathways by influencing the downstream apoptosis cascade required for cell death. It acts as a link bridging initial DNA damage response and the execution of apoptosis providing a control node within these pathways.

Research links PIG3 to cancer and neurodegenerative diseases. In cancer its expression may alter affecting cell apoptosis and proliferation balance and interacting closely with proteins like p53 and BAX. In neurodegenerative disorders abnormal PIG3 activity couples with oxidative stress that leads to neuronal degeneration. Its interplay with p53 remains significant as p53's regulation of apoptotic pathways impacts numerous conditions where PIG3's role becomes equally affected.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

NADPH : quinone reductase which expression is induced by p53/TP53 and is involved in both the DNA damage response through homologous recombination repair, and p53-mediated apoptosis (PubMed : 19349281, PubMed : 33782397). Catalyzes the NADPH-dependent reduction of quinones, exhibiting a low enzymatic activity with beta-naphthoquinones and a strong preference for the ortho-quinone isomer (1,2-beta-naphthoquinone) over the para isomer (1,4-beta-naphthoquinone). Also displays a low reductase activity for non-quinone compounds such as diamine and 2,6-dichloroindophenol (in vitro) (PubMed : 19349281). The reduction of 1,2-naphthoquinone concurrently generates reactive oxygen species (ROS), which may trigger apoptosis downstream of p53/TP53 (PubMed : 19349281).

See full target information TP53I3

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 11:615967 PubMed33777753

2021

Identification of Proteins Deregulated by Platinum-Based Chemotherapy as Novel Biomarkers and Therapeutic Targets in Non-Small Cell Lung Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah-Louise Ryan,Keyur A Dave,Sam Beard,Martina Gyimesi,Matthew McTaggart,Katherine B Sahin,Christopher Molloy,Neha S Gandhi,Eric Boittier,Connor G O'Leary,Esha T Shah,Emma Bolderson,Anne-Marie Baird,Derek J Richard,Kenneth J O'Byrne,Mark N Adams

Oncogene 34:2461-70 PubMed25023704

2014

Ash2L enables P53-dependent apoptosis by favoring stable transcription pre-initiation complex formation on its pro-apoptotic target promoters.

Applications

Unspecified application

Species

Unspecified reactive species

S K Mungamuri,S Wang,J J Manfredi,W Gu,S A Aaronson

Cell death and differentiation 20:576-88 PubMed23306555

2013

A threshold mechanism mediates p53 cell fate decision between growth arrest and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

M Kracikova,G Akiri,A George,R Sachidanandam,S A Aaronson

Nature structural & molecular biology 19:478-84, S1 PubMed22466965

2012

p53-mediated heterochromatin reorganization regulates its cell fate decisions.

Applications

Species

Unspecified reactive species

Sathish Kumar Mungamuri,Erica Kay Benson,Shaomeng Wang,Wei Gu,Sam W Lee,Stuart A Aaronson

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PIG3 antibody

Western blot - Anti-PIG3 antibody (AB64798)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise