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AB96819

Anti-PIG3 antibody

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(2 Publications)

Rabbit Polyclonal PIG3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TP53I3 aa 1 to C-terminus.

View Alternative Names

PIG3, TP53I3, Quinone oxidoreductase PIG3, NADPH:quinone reductase PIG3, Tumor protein p53-inducible protein 3, p53-induced gene 3 protein, Protein PIG3

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PIG3 antibody (AB96819)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PIG3 antibody (AB96819)

Immunofluorescence analysis of paraformaldehyde fixed A549 cells, using ab96819 at 1/200 dilution : (1) without DNA probe; (2) merged with DNA probe.

Western blot - Anti-PIG3 antibody (AB96819)
  • WB

Unknown

Western blot - Anti-PIG3 antibody (AB96819)

10% SDS PAGE

All lanes:

Western blot - Anti-PIG3 antibody (ab96819) at 1/1000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TP53I3 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q53FA7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Post translation modification: Acetylation
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PIG3 also known as PIG00 or P@IG3 str0h is a protein linked to cellular stress response with a molecular mass of approximately 55 kDa. It shows expression mainly in tissues that undergo oxidative stress or DNA damage acting as an important player in apoptosis. PIG3 is mainly known as p53-induced gene 3 aligning with its dependency on p53 for activation. High expression levels become evident in tissues encountering high metabolic or environmental stress.
Biological function summary

PIG3 mediates cell apoptosis in response to DNA damage and oxidative stress. It forms part of a complex with p53 contributing to the regulation of apoptotic pathways. As an oxidoreductase PIG3 initiates pro-apoptotic changes within the cell. Its enzymatic activity involves producing reactive oxygen species (ROS) further amplifying the cell’s apoptotic initiations. Its role connects to cellular response mechanisms in stress conditions dealing with damaged or unhealthy cells.

Pathways

PIG3 operates within the important p53 apoptotic pathway and the DNA damage signaling pathway. It significantly interacts with p53 as PIG3 expression depends on p53 transcription factor activation. Also PIG3 connects with caspase pathways by influencing the downstream apoptosis cascade required for cell death. It acts as a link bridging initial DNA damage response and the execution of apoptosis providing a control node within these pathways.

Research links PIG3 to cancer and neurodegenerative diseases. In cancer its expression may alter affecting cell apoptosis and proliferation balance and interacting closely with proteins like p53 and BAX. In neurodegenerative disorders abnormal PIG3 activity couples with oxidative stress that leads to neuronal degeneration. Its interplay with p53 remains significant as p53's regulation of apoptotic pathways impacts numerous conditions where PIG3's role becomes equally affected.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the NADPH-dependent reduction of quinones (PubMed : 19349281). Exhibits a low enzymatic activity with beta-naphthoquinones, with a strong preference for the ortho-quinone isomer (1,2-beta-naphthoquinone) over the para isomer (1,4-beta-naphthoquinone). Also displays a low reductase activity for non-quinone compounds such as diamine and 2,6-dichloroindophenol (in vitro) (PubMed : 19349281). Involved in the generation of reactive oxygen species (ROS) (PubMed : 19349281).
See full target information TP53I3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

World journal of surgical oncology 19:232 PubMed34362378

2021

Cyanidin-3-glucoside suppresses the progression of lung adenocarcinoma by downregulating TP53I3 and inhibiting PI3K/AKT/mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojun Chen,Weixia Zhang,Xiuzhen Xu

Cell & bioscience 10:121 PubMed33088477

2020

Identification of atypical mitogen-activated protein kinase MAPK4 as a novel regulator in acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Mao,Ya Zhou,Longqing Chen,Lin Hu,Shiming Liu,Wen Zheng,Juanjuan Zhao,Mengmeng Guo,Chao Chen,Zhixu He,Lin Xu
View all publications
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