AB230516

Anti-PiGF antibody - C-terminal

Name: Anti-PiGF antibody - C-terminal (ab230516) | Abcam
Brand: Abcam Limited
SKU: AB230516

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(3 Publications)

Rabbit Polyclonal PIGF antibody. C-terminal. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PIGF conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

GPI11 homolog, Phosphatidylinositol-glycan biosynthesis class F protein, PIG-F, PIGF

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PiGF antibody - C-terminal (AB230516)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PiGF antibody - C-terminal (AB230516)

Formalin-fixed, paraffin-embedded human kidney tissue stained for PiGF using ab230516 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-PiGF antibody - C-terminal (AB230516)

All lanes:

Western blot - Anti-PiGF antibody - C-terminal (ab230516) at 1/500 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 2:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

Lane 3:

H9c2 (rat heart myoblast cell line) whole cell lysate

Predicted band size: 25 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human PIGF conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q07326

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.01% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Placental Growth Factor or PlGF is an important protein involved in angiogenesis and vasculogenesis. This protein with a molecular mass of around 23 kDa belongs to the vascular endothelial growth factor (VEGF) family. Primarily it expresses in the placenta but you can also find it in the heart thyroid and lungs. PlGF interacts with its receptor VEGFR-1 (Flt-1) playing a significant role in blood vessel formation and repair. This makes it an important focus in the study of vascular biology and pathophysiology.

Biological function summary

PlGF enhances cellular proliferation and migration. As a homodimer it binds to VEGFR-1 mediating angiogenic processes that involve endothelial cells. PlGF's role becomes important in the remodeling of vascular tissues especially under pathological conditions where it supports the growth of new blood vessels. Its expression often increases during tissue ischemia indicating its involvement in tissue recovery and regeneration processes.

Pathways

PlGF functions in the angiogenesis signaling pathway working with proteins such as VEGF-A. This pathway is essential for the development of new blood vessels allowing the supply of nutrients and oxygen to tissues especially during growth or repair. PlGF also plays a role in the inflammatory pathway influencing immune cell activation and infiltration. Through these processes PlGF collaborates with other growth factors and cytokines contributing to a complex network of cellular activities required for maintaining vascular health.

PlGF shows relevance to cancer and cardiovascular diseases. The protein contributes to tumor angiogenesis providing tumors with the blood supply needed for growth and metastasis. PlGF also relates to disorders like atherosclerosis where it influences plaque formation and progression in the arteries. In these contexts PlGF often interacts with other members of the VEGF family modulating the pathological environment. Understanding these interactions offers potential therapeutic targets for managing these conditions better.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Stabilizing subunit of the ethanolamine phosphate transferase 3 and ethanolamine phosphate transferase 2 complexes that sequentially transfer an ethanolamine phosphate (EtNP) from a phosphatidylethanolamine (PE) to the 6-OH position of the third alpha-1,2-linked mannose and the second alpha-1,6-linked mannose of a 2-acyl-6-[alpha-D-mannosyl-(1->2)-alpha-D-mannosyl-(1->6)-2-phosphoethanolamine-alpha-D-mannosyl-(1->4)-alpha-D-glucosaminyl]-1-(1-radyl,2-acyl-sn-glycero-3-phospho)-1D-myo-inositol (also termed H6) intermediate to generate a 2-acyl-6-[6-phosphoethanolamine-alpha-D-mannosyl-(1->2)-6-phosphoethanolamine-alpha-D-mannosyl-(1->6)-2-phosphoethanolamine-alpha-D-mannosyl-(1->4)-alpha-D-glucosaminyl]-1-(1-radyl,2-acyl-sn-glycero-3-phospho)-1D-myo-inositol (also termed H8) (PubMed : 15632136). Participates in the tenth and eleventh steps of the glycosylphosphatidylinositol-anchor biosynthesis, in association with PIGO and PIGG, respectively (PubMed : 15632136, PubMed : 33386993).

See full target information PIGF

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 26:99 PubMed33143661

2020

Involvement of Hdac3-mediated inhibition of microRNA cluster 17-92 in bronchopulmonary dysplasia development.

Applications

Unspecified application

Species

Unspecified reactive species

Di Wang,Hui Hong,Xiao-Xia Li,Jing Li,Zhi-Qun Zhang

International journal of molecular sciences 21: PubMed32260158

2020

Increased Angiogenesis and Lymphangiogenesis in the Placental Villi of Women with Chronic Venous Disease during Pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel A Ortega,Miguel A Saez,Oscar Fraile-Martínez,Ángel Asúnsolo,Leonel Pekarek,Coral Bravo,Santiago Coca,Felipe Sainz,Melchor Álvarez- Mon,Julia Buján,Natalio García-Honduvilla

Molecular medicine reports 18:705-714 PubMed29845261

2018

Candesartan targeting of angiotensin II type 1 receptor demonstrates benefits for hypertension in pregnancy via the NF‑κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xudong Zhao,Xietong Wang

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PiGF antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PiGF antibody - C-terminal (AB230516)

Western blot - Anti-PiGF antibody - C-terminal (AB230516)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise