Rabbit Polyclonal PIGP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PIGP aa 100 to C-terminus.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PIGP aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P57054
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Orangutan | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
Target data
Function
Part of the glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex that catalyzes the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol and participates in the first step of GPI biosynthesis.
Alternative names
Phosphatidylinositol N-acetylglucosaminyltransferase subunit P, Down syndrome critical region protein 5, Down syndrome critical region protein C, Phosphatidylinositol-glycan biosynthesis class P protein, PIG-P, DSCRC, DSCR5, NPD010, DCRC, PIGP
Recommended products
Rabbit Polyclonal PIGP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PIGP aa 100 to C-terminus.
Alternative names
Phosphatidylinositol N-acetylglucosaminyltransferase subunit P, Down syndrome critical region protein 5, Down syndrome critical region protein C, Phosphatidylinositol-glycan biosynthesis class P protein, PIG-P, DSCRC, DSCR5, NPD010, DCRC, PIGP
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PIGP aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P57054
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PIGP also known as phosphatidylinositol glycan anchor biosynthesis class P acts mechanically in the early step of glycosylphosphatidylinositol (GPI) anchor biosynthesis. This protein plays an essential role in the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to phosphatidylinositol (PI) on the cytoplasmic side of the endoplasmic reticulum. PIGP has an estimated molecular mass of around 10 kDa. The gene encoding PIGP expresses predominantly in several tissues especially in the brain and other neural tissues.
- Biological function summary
PIGP functions as an important component of the GPI transamidase complex—an important part of the GPI anchor biosynthesis process. This complex includes several other proteins such as PIGQ PIGT PIGK and GPAA1 which coordinate closely for GPI anchor synthesis. GPI anchors attach proteins to the cell membrane affecting their localization and function. Therefore PIGP contributes significantly to diverse cellular mechanisms.
- Pathways
PIGP participates integrally in the GPI-anchor biosynthesis pathway and the inositol phosphate metabolism pathway. It closely interacts with other proteins like PIGA and PIGC that are involved in the same biosynthetic pathway. These interactions ensure the proper assembly and function of GPI-anchored proteins which play substantial roles in signal transduction and cell adhesion processes.
- Associated diseases and disorders
PIGP has associations with inherited conditions such as Paroxysmal Nocturnal Hemoglobinuria (PNH) and Down syndrome-related mental disorders. In PNH aberrations in the GPI-anchor biosynthesis disrupt cell membrane stability often involving proteins like PIGA. Meanwhile specific mutations within this pathway may link to intellectual disabilities seen in Down syndrome illustrating the clinical significance of PIGP and related proteins in human health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-PIGP antibody (ab224128)
All lanes: Western blot - Anti-PIGP antibody (ab224128) at 1/100 dilution
Lane 1: Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2: PIGP overexpressing HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Developed using the ECL technique.
Predicted band size: 18 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIGP antibody (ab224128)
Paraffin-embedded human stomach tissue stained for PIGP using ab224128 at 1/50 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-PIGP antibody (ab224128)
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for PIGP (green) using ab224128 at 4 μg/ml in ICC/IF.
Downloads
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