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AB224128

Anti-PIGP antibody

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(1 Publication)

Rabbit Polyclonal PIGP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PIGP aa 100 to C-terminus.

View Alternative Names

DCRC, DSCR5, DSCRC, NPD010, PIGP, Phosphatidylinositol N-acetylglucosaminyltransferase subunit P, Down syndrome critical region protein 5, Down syndrome critical region protein C, Phosphatidylinositol-glycan biosynthesis class P protein, PIG-P

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-PIGP antibody (AB224128)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PIGP antibody (AB224128)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for PIGP (green) using ab224128 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIGP antibody (AB224128)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIGP antibody (AB224128)

Paraffin-embedded human stomach tissue stained for PIGP using ab224128 at 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-PIGP antibody (AB224128)
  • WB

Supplier Data

Western blot - Anti-PIGP antibody (AB224128)

All lanes:

Western blot - Anti-PIGP antibody (ab224128) at 1/100 dilution

Lane 1:

Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 2:

PIGP overexpressing HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Predicted band size: 18 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human PIGP aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P57054

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PIGP also known as phosphatidylinositol glycan anchor biosynthesis class P acts mechanically in the early step of glycosylphosphatidylinositol (GPI) anchor biosynthesis. This protein plays an essential role in the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc to phosphatidylinositol (PI) on the cytoplasmic side of the endoplasmic reticulum. PIGP has an estimated molecular mass of around 10 kDa. The gene encoding PIGP expresses predominantly in several tissues especially in the brain and other neural tissues.
Biological function summary

PIGP functions as an important component of the GPI transamidase complex—an important part of the GPI anchor biosynthesis process. This complex includes several other proteins such as PIGQ PIGT PIGK and GPAA1 which coordinate closely for GPI anchor synthesis. GPI anchors attach proteins to the cell membrane affecting their localization and function. Therefore PIGP contributes significantly to diverse cellular mechanisms.

Pathways

PIGP participates integrally in the GPI-anchor biosynthesis pathway and the inositol phosphate metabolism pathway. It closely interacts with other proteins like PIGA and PIGC that are involved in the same biosynthetic pathway. These interactions ensure the proper assembly and function of GPI-anchored proteins which play substantial roles in signal transduction and cell adhesion processes.

PIGP has associations with inherited conditions such as Paroxysmal Nocturnal Hemoglobinuria (PNH) and Down syndrome-related mental disorders. In PNH aberrations in the GPI-anchor biosynthesis disrupt cell membrane stability often involving proteins like PIGA. Meanwhile specific mutations within this pathway may link to intellectual disabilities seen in Down syndrome illustrating the clinical significance of PIGP and related proteins in human health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the glycosylphosphatidylinositol-N-acetylglucosaminyltransferase (GPI-GnT) complex that catalyzes the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to phosphatidylinositol and participates in the first step of GPI biosynthesis.
See full target information PIGP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

microPublication biology 2025: PubMed41050330

2025

Cells stably expressing shRNA against MYO10 display altered cell motility.

Applications

Unspecified application

Species

Unspecified reactive species

Joanna A Mas,Chase E Cristella,Vu Hao M N Phan,Lillian S Wendt,Charlotte A Rose,Abigail Ali,David F Carpio,Christine Cole,Paige Embley,Jack E Hoskins-Harris,Delia Johnson,Noelle Ledoux,Hannah W Lwin,Sarah Salah,Erin Weisbart,Stacey J Criswell,Omar Alberto Quintero-Carmona
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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