AB157211

Anti-PIGS antibody [EPR11275(B)]

Name: Anti-PIGS antibody [EPR11275(B)] (ab157211) | Abcam
Brand: Abcam Limited
SKU: AB157211
Price: 370 USD
Availability: InStock
Rating: 3 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(2 Publications)

Rabbit Recombinant Monoclonal PIGS antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

UNQ1873/PRO4316, PIGS, GPI transamidase component PIG-S, Phosphatidylinositol-glycan biosynthesis class S protein

2 Images

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Immunoprecipitation - Anti-PIGS antibody [EPR11275(B)] (AB157211)

Immunoprecipitation analysis of pellet from HeLa cell lysate labeling PIGS using ab157211 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-PIGS antibody [EPR11275(B)] (ab157211) at 1/1000 dilution

All lanes:

Immunoprecipitation pellet from HeLa cell lysat at 10 µg

Predicted band size: 62 kDa

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Western blot - Anti-PIGS antibody [EPR11275(B)] (AB157211)

All lanes:

Western blot - Anti-PIGS antibody [EPR11275(B)] (ab157211) at 1/1000 dilution

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PC-3 cell lysate at 10 µg

Lane 2:

Fetal liver cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

A431 cell lysate at 10 µg

Predicted band size: 62 kDa

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Carrier free

Anti-PIGS antibody [EPR11275(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11275(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PIGS also known as Phosphatidylinositol Glycan Anchor Biosynthesis Class S is a protein involved in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors. It is a component of the GPI transamidase complex which plays an important role in attaching GPI anchors to proteins. PIGS has a molecular mass of approximately 99 kDa and is predominantly expressed in the endoplasmic reticulum. This protein ensures the proper anchoring of GPI-anchored proteins to the membrane which is essential for their function.

Biological function summary

The GPI anchor system is critical for membrane protein localization and function. PIGS is an integral part of a multimeric enzyme complex within the endoplasmic reticulum that facilitates the attachment of GPI anchors to proteins. This process affects various cellular mechanisms including signal transduction and cell adhesion. Proper function of PIGS and its associated complex is necessary for maintaining the stability and activity of GPI-anchored proteins.

Pathways

The role of PIGS is important in the GPI-anchor biosynthesis pathway. This pathway involves a series of reactions that result in the addition of a GPI anchor to proteins facilitating their sorting and targeting to specific cellular locations. PIGS is part of this pathway along with other proteins like GPAA1 and PIGT which work together in the GPI transamidase complex to attach newly synthesized GPI anchors to proteins.

Malfunction of PIGS or disruptions in GPI anchor biosynthesis can lead to health issues such as paroxysmal nocturnal hemoglobinuria (PNH) and hyperphosphatasia with mental retardation syndrome (HPMRS). In PNH defects in GPI-anchor synthesis lead to increased sensitivity of red blood cells to complement-mediated lysis. PIGS interacts with proteins such as CD59 and DAF which rely on GPI anchors to inhibit complement pathway activation and ensure cell protection.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the GPI transamidase complex. Essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates.

See full target information PIGS

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer discovery 13:1186-1209 PubMed36811466

2023

Addressing Tumor Heterogeneity by Sensitizing Resistant Cancer Cells to T cell-Secreted Cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshinaga Ito,Deng Pan,Wubing Zhang,Xixi Zhang,Tiffany Y Juan,Jason W Pyrdol,Oleksandr Kyrysyuk,John G Doench,X Shirley Liu,Kai W Wucherpfennig

Molecular medicine reports 18:684-694 PubMed29767244

2018

Propranolol induces hemangioma endothelial cell apoptosis via a p53‑BAX mediated pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tian-Hua Yao,Parekejiang Pataer,Krishna Prasad Regmi,Xi-Wen Gu,Quan-Yan Li,Jing-Ting Du,Su-Meng Ge,Jun-Bo Tu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PIGS antibody [EPR11275(B)]

Immunoprecipitation - Anti-PIGS antibody [EPR11275(B)] (AB157211)

Western blot - Anti-PIGS antibody [EPR11275(B)] (AB157211)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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Product promise