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AB249313

Anti-PIGS antibody [EPR11275(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal PIGS antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

View Alternative Names

UNQ1873/PRO4316, PIGS, GPI transamidase component PIG-S, Phosphatidylinositol-glycan biosynthesis class S protein

2 Images
Immunoprecipitation - Anti-PIGS antibody [EPR11275(B)] - BSA and Azide free (AB249313)
  • IP

Unknown

Immunoprecipitation - Anti-PIGS antibody [EPR11275(B)] - BSA and Azide free (AB249313)

This data was developed using ab157211, the same antibody clone in a different buffer formulation.

Immunoprecipitation analysis of pellet from HeLa cell lysate labeling PIGS using ab157211 at 1/10 dilution.

All lanes:

Immunoprecipitation - Anti-PIGS antibody [EPR11275(B)] (<a href='/en-us/products/primary-antibodies/pigs-antibody-epr11275b-ab157211'>ab157211</a>) at 1/1000 dilution

All lanes:

Immunoprecipitation pellet from HeLa cell lysat at 10 µg

Predicted band size: 62 kDa

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Western blot - Anti-PIGS antibody [EPR11275(B)] - BSA and Azide free (AB249313)
  • WB

Unknown

Western blot - Anti-PIGS antibody [EPR11275(B)] - BSA and Azide free (AB249313)

This data was developed using ab157211, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PIGS antibody [EPR11275(B)] (<a href='/en-us/products/primary-antibodies/pigs-antibody-epr11275b-ab157211'>ab157211</a>) at 1/1000 dilution

Lane 1:

PC-3 cell lysate at 10 µg

Lane 2:

Fetal liver cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

A431 cell lysate at 10 µg

Predicted band size: 62 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11275(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

ab249313 is the carrier-free version of ab157211.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PIGS also known as Phosphatidylinositol Glycan Anchor Biosynthesis Class S is a protein involved in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors. It is a component of the GPI transamidase complex which plays an important role in attaching GPI anchors to proteins. PIGS has a molecular mass of approximately 99 kDa and is predominantly expressed in the endoplasmic reticulum. This protein ensures the proper anchoring of GPI-anchored proteins to the membrane which is essential for their function.
Biological function summary

The GPI anchor system is critical for membrane protein localization and function. PIGS is an integral part of a multimeric enzyme complex within the endoplasmic reticulum that facilitates the attachment of GPI anchors to proteins. This process affects various cellular mechanisms including signal transduction and cell adhesion. Proper function of PIGS and its associated complex is necessary for maintaining the stability and activity of GPI-anchored proteins.

Pathways

The role of PIGS is important in the GPI-anchor biosynthesis pathway. This pathway involves a series of reactions that result in the addition of a GPI anchor to proteins facilitating their sorting and targeting to specific cellular locations. PIGS is part of this pathway along with other proteins like GPAA1 and PIGT which work together in the GPI transamidase complex to attach newly synthesized GPI anchors to proteins.

Malfunction of PIGS or disruptions in GPI anchor biosynthesis can lead to health issues such as paroxysmal nocturnal hemoglobinuria (PNH) and hyperphosphatasia with mental retardation syndrome (HPMRS). In PNH defects in GPI-anchor synthesis lead to increased sensitivity of red blood cells to complement-mediated lysis. PIGS interacts with proteins such as CD59 and DAF which rely on GPI anchors to inhibit complement pathway activation and ensure cell protection.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the GPI transamidase complex. Essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates.
See full target information PIGS
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com