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AB237800

Anti-PIGT antibody

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(2 Publications)

Rabbit Polyclonal PIGT antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PIGT aa 400 to C-terminus.

View Alternative Names

CGI-06, PSEC0163, UNQ716/PRO1379, PIGT, GPI transamidase component PIG-T, Phosphatidylinositol-glycan biosynthesis class T protein

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIGT antibody (AB237800)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIGT antibody (AB237800)

Paraffin-embedded human brain tissue stained for PIGT using ab237800 at 1/500 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PIGT aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q969N2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PIGT (Phosphatidylinositol Glycan Anchor Biosynthesis Class T) plays a mechanical role in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors. Also known as PIG-T this protein is part of the GPI transamidase complex and has a molecular mass of approximately 68 kDa. It resides in the endoplasmic reticulum where it supports the attachment of various proteins to the cell membrane through GPI anchoring. PIGT is ubiquitously expressed across different human tissues indicating its fundamental role in cellular physiology.
Biological function summary

PIGT acts as a subunit within the GPI transamidase complex that enhances the transfer of pre-assembled GPI units to proteins. This activity anchors proteins to the cell surface which is necessary for their stability and function. The GPI anchor allows effective signaling adhesion and protection of proteins impacting many cellular processes. The interdependency with other subunits such as PIGA PIGK and PIGS ensures the complete function of the GPI transamidase complex.

Pathways

PIGT remains key within the GPI-anchor biosynthesis pathway playing a critical role in cell surface protein localization. It interacts with other members like PIGU and GAA1 within this pathway forming interactions necessary for proper transit and attachment of GPI-anchored proteins. The functionality of the GPI-anchor pathway directly influences cellular communication through pathways like signal transduction which are essential for different biological processes.

Mutations or deficiencies in PIGT link to Paroxysmal Nocturnal Hemoglobinuria (PNH) and Early Infantile Epileptic Encephalopathy (EIEE). PNH involves defective GPI-anchored proteins affecting the regulation of cell membrane stability while EIEE relates to neurological complications due to disturbed protein functioning. PIGT connects with other proteins like PIGM and PIGL contributing to the disorders' pathophysiology when disruptions in GPI-anchor biosynthesis occur.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the GPI transamidase complex. Essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates.
See full target information PIGT
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 22:5 PubMed38169393

2024

PIGT promotes cell growth, glycolysis, and metastasis in bladder cancer by modulating GLUT1 glycosylation and membrane trafficking.

Applications

Unspecified application

Species

Unspecified reactive species

Mingyue Tan,Qi Pan,Chao Yu,Xinyu Zhai,Jianyi Gu,Le Tao,Dongliang Xu

Molecular therapy. Nucleic acids 22:815-831 PubMed33230478

2020

Circular RNA circRIMS1 Acts as a Sponge of miR-433-3p to Promote Bladder Cancer Progression by Regulating CCAR1 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Feifan Wang,Mengjing Fan,Yueshu Cai,Xuejian Zhou,Shengcheng Tai,Yanlan Yu,Hongshen Wu,Yan Zhang,Jiaxin Liu,Shihan Huang,Ning He,Zhenghui Hu,Xiaodong Jin
View all publications
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