Rabbit Polyclonal PIGV antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PIGV aa 400 to C-terminus.
View Alternative Names
GPI mannosyltransferase 2, GPI mannosyltransferase II, Phosphatidylinositol-glycan biosynthesis class V protein, GPI-MT-II, PIG-V, PIGV
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIGV antibody (AB237692)
Paraffin-embedded human kidney tissue stained for PIGV using ab237692 at 1/200 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
- WB
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Western blot - Anti-PIGV antibody (AB237692)
All lanes:
Western blot - Anti-PIGV antibody (ab237692) at 1/500 dilution
All lanes:
A549 (human lung carcinoma cell line) whole cell lysate
Secondary
All lanes:
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 55 kDa
false
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-PIGV antibody (AB237692)
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PIGV (Green) using ab237692 at 1/66 dilution in ICC/IF, followed by Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.
Reactivity data
Properties and storage information
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Supplementary information
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Biological function summary
The protein PIGV participates in the GPI anchor biosynthesis pathway which is vital for the post-translational modification of proteins. This protein acts as part of a GPI-mannosyltransferase II complex within the endoplasmic reticulum. The complex adds mannose to the growing GPI anchor an important step for anchoring proteins to the cell surface. Such anchored proteins are often involved in cell signaling immune response and interactions between cells.
Pathways
The PIGV protein functions within the broader GPI-anchor biosynthesis pathway. It contributes directly to the synthesis of the GPI-anchor component critical for cellular processes involving surface proteins. The PIGV enzyme operates alongside PIGO and other GPI-related proteins in this pathway ensuring the precise assembly of the GPI-anchor which is necessary for the anchoring function.
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Target data
Publications (2)
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The Journal of biological chemistry 299:104982 PubMed37390992
2023
Applications
Unspecified application
Species
Unspecified reactive species
Cancer medicine 12:4968-4980 PubMed36056909
2022
Applications
Unspecified application
Species
Unspecified reactive species
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