Rabbit Polyclonal PIKE antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Immunogen corresponding to Synthetic Peptide within Human Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2. The exact immunogen used to generate this antibody is proprietary information. Database link Q99490
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Mouse | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.50000-1.00000 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 10 µg/mL | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion.
Alternative names
AGAP-2, Centaurin-gamma-1, GTP-binding and GTPase-activating protein 2, Phosphatidylinositol 3-kinase enhancer, Cnt-g1, GGAP2, PIKE, AGAP2, CENTG1, KIAA0167
Recommended products
Rabbit Polyclonal PIKE antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Immunogen corresponding to Synthetic Peptide within Human Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2. The exact immunogen used to generate this antibody is proprietary information. Database link Q99490
- Purification technique
Affinity purification Protein A
- Specificity
Recognises PIKE: also detect both PIKE-L and PIKE-A isoforms.
- Concentration
- Purification notes
Purified by ion exchange chromatography.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-PIKE antibody (ab21271)
ab21271 at concentrations of 0.5 (lane A), 1 (lane B) and 2 μg/ml (lane C), staining PIKE-L and PIKE-A in mouse brain cell lysates by Western blot.
Lane 1: Western blot - Anti-PIKE antibody (ab21271) at 0.5 µg/mL
Lane 2: Western blot - Anti-PIKE antibody (ab21271) at 1 µg/mL
Lane 3: Western blot - Anti-PIKE antibody (ab21271) at 2 µg/mL
All lanes: Mouse brain cell lysate
Predicted band size: 124 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIKE antibody (ab21271)
ab21271 at 10μg/ml staining PIKE in mouse brain tissue by IHC
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