Rabbit Polyclonal PIKE antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AGAP2 aa 700-850.
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Publications
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- Cellular and molecular gastroenterology and hepatology 15:533-5582022Hepatitis B Virus Utilizes a Retrograde Trafficking Route via the Trans-Golgi Network to Avoid Lysosomal Degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing-Yi Li,Kazuyuki Kuroki,Tetsuro Shimakami,Kazuhisa Murai,Kazunori Kawaguchi,Takayoshi Shirasaki,Kouki Nio,Saiho Sugimoto,Tomoki Nishikawa,Hikari Okada,Noriaki Orita,Hideo Takayama,Ying Wang,Phuong Doan Thi Bich,Astuya Ishida,Sadahiro Iwabuchi,Shinichi Hashimoto,Takeshi Shimaoka,Noriko Tabata,Miho Watanabe-Takahashi,Kiyotaka Nishikawa,Hiroshi Yanagawa,Motoharu Seiki,Kouji Matsushima,Taro Yamashita,Shuichi Kaneko,Masao HondaPubMed 36270602
- Aging 11:5300-53182019Exosomes derived from microRNA-199a-overexpressing mesenchymal stem cells inhibit glioma progression by down-regulating AGAP2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Yu et. al.PubMed 31386624
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human AGAP2 aa 700-850. The exact immunogen used to generate this antibody is proprietary information. Database link Q99490
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and also seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1)-dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion.
Alternative names
CENTG1, KIAA0167, AGAP2, AGAP-2, Centaurin-gamma-1, GTP-binding and GTPase-activating protein 2, Phosphatidylinositol 3-kinase enhancer, Cnt-g1, GGAP2, PIKE
Recommended products
Rabbit Polyclonal PIKE antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AGAP2 aa 700-850.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human AGAP2 aa 700-850. The exact immunogen used to generate this antibody is proprietary information. Database link Q99490
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PIKE also known as phosphoinositide 3-kinase enhancer is a protein encoded by the CENTG1 gene. It weighs approximately 97 kDa and has several isoforms including PIKE-A PIKE-L and PIKE-S each with distinct localizations and functions. PIKE is expressed in neural tissues especially in the brain where it regulates numerous signaling processes. This protein facilitates the activation of phosphoinositide 3-kinase (PI3K) by directly interacting with it thereby enhancing downstream signaling.
- Biological function summary
Proteins like PIKE are important in regulating cellular growth survival and proliferation. PIKE acts as an important mediator in the PI3K signaling pathway and is known to affect neuronal cell development and neurite outgrowth. It participates in forming complexes with other proteins such as Akt further amplifying signaling cascades linked to cell survival and growth. Its prominent role in neural systems makes PIKE significant in maintaining normal brain function.
- Pathways
The protein PIKE integrates significantly into the PI3K/Akt signaling pathway and the small GTPase pathway. In the PI3K/Akt pathway PIKE works closely with Akt and mTOR proteins promoting cell survival and growth. In the small GTPase pathway PIKE interacts with proteins like Rac1 which regulates actin cytoskeleton dynamics and influences changes in cell shape and motility. These pathways highlight PIKE's involvement in essential cellular processes.
- Associated diseases and disorders
The abnormal regulation of PIKE links to specific conditions such as cancer and schizophrenia. In cancer altered PIKE activity contributes to tumorigenesis through its interaction with growth-related proteins including Akt. In schizophrenia changes in PIKE expression or function can affect neural signaling pathways potentially influencing cognitive and behavioral symptoms associated with the disorder. Understanding PIKE's role in these conditions could aid in developing new therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-PIKE antibody (ab224118)
PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for PIKE (green) using ab224118 (4 μg/ml) in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIKE antibody (ab224118)
Paraffin-embedded human cerebellum tissue stained for PIKE with ab224118 (1/50) in immunohistochemical analysis.
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