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AB300454

Anti-PIM1 antibody [EPR24447-39] - BSA and Azide free

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Rabbit Recombinant Monoclonal PIM1 antibody. Carrier free. Suitable for WB, IP and reacts with Human, Mouse samples.

View Alternative Names

Serine/threonine-protein kinase pim-1, PIM1

4 Images
Western blot - Anti-PIM1 antibody [EPR24447-39] - BSA and Azide free (AB300454)
  • WB

Supplier Data

Western blot - Anti-PIM1 antibody [EPR24447-39] - BSA and Azide free (AB300454)

This data was developed using ab300453, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PIM1 antibody [EPR24447-39] (<a href='/en-us/products/primary-antibodies/pim1-antibody-epr24447-39-ab300453'>ab300453</a>) at 1/5000 dilution

Lane 1:

His-tagged human PIM1 recombinant protein at 0.01 µg

Lane 2:

His-tagged human PIM3 recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 45 kDa

Observed band size: 44 kDa

false

Exposure time: 7s

Western blot - Anti-PIM1 antibody [EPR24447-39] - BSA and Azide free (AB300454)
  • WB

Supplier Data

Western blot - Anti-PIM1 antibody [EPR24447-39] - BSA and Azide free (AB300454)

This data was developed using ab300453, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PIM1 antibody [EPR24447-39] (<a href='/en-us/products/primary-antibodies/pim1-antibody-epr24447-39-ab300453'>ab300453</a>) at 1/1000 dilution

Lane 1:

K-562 transfected with scrambled siRNA control, whole cell lysate at 20 µg

Lane 2:

K-562 transfected with siRNA specifically targeting PIM1, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 45 kDa

Observed band size: 34 kDa

false

Exposure time: 37s

Western blot - Anti-PIM1 antibody [EPR24447-39] - BSA and Azide free (AB300454)
  • WB

Supplier Data

Western blot - Anti-PIM1 antibody [EPR24447-39] - BSA and Azide free (AB300454)

This data was developed using ab300453, the same antibody clone in a different buffer formulation.

Blocking / Diluting buffer and concentration : 5% NFDM/TBST

Exposure time :

Lane 1, 2 : 70 seconds

Lane 3 : 3 minutes

All lanes:

Western blot - Anti-PIM1 antibody [EPR24447-39] (<a href='/en-us/products/primary-antibodies/pim1-antibody-epr24447-39-ab300453'>ab300453</a>) at 1/1000 dilution

Lane 1:

K-562 whole cell lysate at 20 µg

Lane 2:

A20 whole cell lysate at 20 µg

Lane 3:

F9 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 45 kDa

Observed band size: 34 kDa

false

Immunoprecipitation - Anti-PIM1 antibody [EPR24447-39] - BSA and Azide free (AB300454)
  • IP

Supplier Data

Immunoprecipitation - Anti-PIM1 antibody [EPR24447-39] - BSA and Azide free (AB300454)

This data was developed using ab300454, the same antibody clone in a different buffer formulation. PIM1 was immunoprecipitated from 0.35 mg A20 (Mouse reticulum sarcoma B lymphocyte), whole cell lysate 10 ug with ab300453 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300453 at ID63 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : A20 (Mouse reticulum sarcoma B lymphocyte), whole cell lysate 10 ug Lane 2 : ab300453 IP in A20 whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab300453 in A20 whole cell lysate. Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 3 minutes

Lane 1:

Immunoprecipitation - Anti-PIM1 antibody [EPR24447-39] (<a href='/en-us/products/primary-antibodies/pim1-antibody-epr24447-39-ab300453'>ab300453</a>) at 1/30 dilution

Lane 2:

Immunoprecipitation - Anti-PIM1 antibody [EPR24447-39] (<a href='/en-us/products/primary-antibodies/pim1-antibody-epr24447-39-ab300453'>ab300453</a>) at 1/1000 dilution

All lanes:

A20 whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24447-39

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PIM1 protein also known as Proto-oncogene serine/threonine-protein kinase Pim-1 is a serine/threonine kinase with a mass of approximately 44 kDa. PIM1 is expressed ubiquitously in the body but shows higher expression levels in hematopoietic tissues such as spleen thymus and bone marrow. Within the cell PIM1 predominantly locates to the cytoplasm but can also be found in the nucleus. The PIM1 protein contains conserved kinase domains which are essential for its catalytic activity.
Biological function summary

PIM1 protein plays an important role in cell survival proliferation and differentiation. It functions as a regulator within signal transduction pathways and although not forming part of a larger complex its kinase activity affects other proteins' phosphorylation status influencing various cellular processes. High levels of PIM1 expression are often associated with increased cellular proliferation and are involved in feedback loops enhancing cell survival.

Pathways

PIM1 protein contributes significantly to the JAK/STAT and PI3K/AKT signaling pathways which are important for the regulation of immune responses and cell growth. Within these pathways PIM1 interacts with other proteins such as STAT5 and AKT mediating signals that promote cell cycle progression and apoptosis inhibition. Its role is particularly important for the modulation of hematopoietic and immune functions where it facilitates cellular responses to external growth stimuli.

PIM1 protein is implicated in several cancers notably hematological malignancies like leukemia and lymphomas. The overexpression of PIM1 cooperates with oncogenes like MYC and BCL2 contributing to tumorigenesis by promoting uncontrolled cell proliferation and survival. Additionally PIM1 is under investigation for its role in cardiovascular diseases where it may impact cardiomyocyte survival and heart repair mechanisms. Targeting PIM1 and its associated pathways may offer therapeutic potentials in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis (PubMed : 15528381, PubMed : 1825810, PubMed : 31548394). Exerts its oncogenic activity through : the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3) (PubMed : 18593906). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity (By similarity). The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis (By similarity). Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1 (By similarity). Phosphorylation of MAP3K5, another proapoptotic protein, by PIM1, significantly decreases MAP3K5 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis (PubMed : 19749799). Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C (PubMed : 16356754). Phosphorylation of CDKN1A, a regulator of cell cycle progression at G1, results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability (PubMed : 12431783). Promotes cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, CDKN1B, at both transcriptional and post-translational levels (PubMed : 18593906). Phosphorylation of CDKN1B, induces 14-3-3 proteins binding, nuclear export and proteasome-dependent degradation (PubMed : 18593906). May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3 (PubMed : 10664448). Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis (By similarity). Acts as a positive regulator of mTORC1 signaling by mediating phosphorylation and inhibition of DEPDC5 component of the GATOR1 complex (PubMed : 31548394). Acts as a negative regulator of innate immunity by mediating phosphorylation and inactivation of GBP1 in absence of infection : phosphorylation of GBP1 induces interaction with 14-3-3 protein sigma (SFN) and retention in the cytosol (PubMed : 37797010). Also phosphorylates and activates the ATP-binding cassette transporter ABCG2, allowing resistance to drugs through their excretion from cells (PubMed : 18056989). Promotes brown adipocyte differentiation (By similarity).
See full target information PIM1
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Product promise

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