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Mouse Monoclonal PIM1 antibody. Suitable for WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human PIM1.

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Images

Western blot - Anti-PIM1 antibody [ZP003] (AB54503), expandable thumbnail
  • Western blot - Anti-PIM1 antibody [ZP003] (AB54503), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human PIM1. The exact immunogen used to generate this antibody is proprietary information. Database link P11309

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested

Tested
Tested

Species
Human
Dilution info
1.00000-3.00000 µg/mL
Notes

-

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Target data

Function

Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation and thus providing a selective advantage in tumorigenesis (PubMed:15528381, PubMed:1825810, PubMed:31548394). Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression and by phosphorylation and inhibition of proapoptotic proteins (BAD, MAP3K5, FOXO3) (PubMed:18593906). Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase of transcriptional activity (By similarity). The stabilization of MYC exerted by PIM1 might explain partly the strong synergism between these two oncogenes in tumorigenesis (By similarity). Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1 (By similarity). Phosphorylation of MAP3K5, another proapoptotic protein, by PIM1, significantly decreases MAP3K5 kinase activity and inhibits MAP3K5-mediated phosphorylation of JNK and JNK/p38MAPK subsequently reducing caspase-3 activation and cell apoptosis (PubMed:19749799). Stimulates cell cycle progression at the G1-S and G2-M transitions by phosphorylation of CDC25A and CDC25C (PubMed:16356754). Phosphorylation of CDKN1A, a regulator of cell cycle progression at G1, results in the relocation of CDKN1A to the cytoplasm and enhanced CDKN1A protein stability (PubMed:12431783). Promotes cell cycle progression and tumorigenesis by down-regulating expression of a regulator of cell cycle progression, CDKN1B, at both transcriptional and post-translational levels (PubMed:18593906). Phosphorylation of CDKN1B, induces 14-3-3 proteins binding, nuclear export and proteasome-dependent degradation (PubMed:18593906). May affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3 (PubMed:10664448). Acts also as a regulator of homing and migration of bone marrow cells involving functional interaction with the CXCL12-CXCR4 signaling axis (By similarity). Acts as a positive regulator of mTORC1 signaling by mediating phosphorylation and inhibition of DEPDC5 component of the GATOR1 complex (PubMed:31548394). Acts as a negative regulator of innate immunity by mediating phosphorylation and inactivation of GBP1 in absence of infection: phosphorylation of GBP1 induces interaction with 14-3-3 protein sigma (SFN) and retention in the cytosol (PubMed:37797010). Also phosphorylates and activates the ATP-binding cassette transporter ABCG2, allowing resistance to drugs through their excretion from cells (PubMed:18056989). Promotes brown adipocyte differentiation (By similarity).

Alternative names

Serine/threonine-protein kinase pim-1, PIM1

Recommended products

Mouse Monoclonal PIM1 antibody. Suitable for WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human PIM1.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human PIM1. The exact immunogen used to generate this antibody is proprietary information. Database link P11309
Clone number
ZP003
Purification technique
Affinity purification Protein A
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The PIM1 protein also known as Proto-oncogene serine/threonine-protein kinase Pim-1 is a serine/threonine kinase with a mass of approximately 44 kDa. PIM1 is expressed ubiquitously in the body but shows higher expression levels in hematopoietic tissues such as spleen thymus and bone marrow. Within the cell PIM1 predominantly locates to the cytoplasm but can also be found in the nucleus. The PIM1 protein contains conserved kinase domains which are essential for its catalytic activity.

Biological function summary

PIM1 protein plays an important role in cell survival proliferation and differentiation. It functions as a regulator within signal transduction pathways and although not forming part of a larger complex its kinase activity affects other proteins' phosphorylation status influencing various cellular processes. High levels of PIM1 expression are often associated with increased cellular proliferation and are involved in feedback loops enhancing cell survival.

Pathways

PIM1 protein contributes significantly to the JAK/STAT and PI3K/AKT signaling pathways which are important for the regulation of immune responses and cell growth. Within these pathways PIM1 interacts with other proteins such as STAT5 and AKT mediating signals that promote cell cycle progression and apoptosis inhibition. Its role is particularly important for the modulation of hematopoietic and immune functions where it facilitates cellular responses to external growth stimuli.

Associated diseases and disorders

PIM1 protein is implicated in several cancers notably hematological malignancies like leukemia and lymphomas. The overexpression of PIM1 cooperates with oncogenes like MYC and BCL2 contributing to tumorigenesis by promoting uncontrolled cell proliferation and survival. Additionally PIM1 is under investigation for its role in cardiovascular diseases where it may impact cardiomyocyte survival and heart repair mechanisms. Targeting PIM1 and its associated pathways may offer therapeutic potentials in these conditions.

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2 product images

  • Western blot - Anti-PIM1 antibody [ZP003] (ab54503), expandable thumbnail

    Western blot - Anti-PIM1 antibody [ZP003] (ab54503)

    Lane 1: Wild-type HAP1 cell lysate (20 μg)
    Lane 2: PIM1 knockout HAP1 cell lysate (20 μg)
    Lane 3: K562 cell lysate (20 μg)
    Lane 4: Jurkat cell lysate (20 μg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab54503 observed at 35 kDa. Red - loading control, Anti-GAPDH antibody [EPR16891] - Loading Control ab181602, observed at 37 kDa.
    ab54503 was shown to recognize PIM1 when PIM1 knockout samples were used, along with additional cross-reactive bands. Wild-type and PIM1 knockout samples were subjected to SDS-PAGE. ab54503 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (loading control to GAPDH) were diluted 3 μg/mL and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-PIM1 antibody [ZP003] (ab54503)

    Predicted band size: 45 kDa

  • Western blot - Anti-PIM1 antibody [ZP003] (ab54503), expandable thumbnail

    Western blot - Anti-PIM1 antibody [ZP003] (ab54503)

    All lanes: Western blot - Anti-PIM1 antibody [ZP003] (ab54503) at 3 µg/mL

    All lanes: K562 cell lysate

    Predicted band size: 45 kDa

    Observed band size: 35 kDa

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