JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB76309

Anti-Pin1 antibody [EP1479Y]

5

(5 Reviews)

|

(7 Publications)

Rabbit Recombinant Monoclonal PIN1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 7 publications.

View Alternative Names

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, Peptidyl-prolyl cis-trans isomerase Pin1, Rotamase Pin1, PPIase Pin1, PIN1

3 Images
Western blot - Anti-Pin1 antibody [EP1479Y] (AB76309)
  • WB

Unknown

Western blot - Anti-Pin1 antibody [EP1479Y] (AB76309)

All lanes:

Western blot - Anti-Pin1 antibody [EP1479Y] (ab76309) at 1/500 dilution

All lanes:

HEK-293 cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 18 kDa

Observed band size: 18 kDa

false

Western blot - Anti-Pin1 antibody [EP1479Y] (AB76309)
  • WB

Lab

Western blot - Anti-Pin1 antibody [EP1479Y] (AB76309)

All lanes:

Western blot - Anti-Pin1 antibody [EP1479Y] (ab76309) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 3:

Mouse brain lysate at 20 µg

Lane 4:

Rat brain lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 18 kDa

Observed band size: 18 kDa

false

Western blot - Anti-Pin1 antibody [EP1479Y] (AB76309)
  • WB

Lab

Western blot - Anti-Pin1 antibody [EP1479Y] (AB76309)

Western blot : Rabbit Monoclonal[EP1479Y] to Pin1 ab76309 staining at 1/1000 dilution, shown in green; Mouse anti alpha Tubulin (ab7291) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at kDa in Wild-type HeLa ab271142 cell lysates with no signal observed at this size in PIN1 knockout HeLa ab273851 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Pin1 antibody [EP1479Y] (ab76309) at 1/1000 dilution

Lane 1:

Wild-type HeLa ab271142 at 20 µg

Lane 2:

Western blot - Human PIN1 knockout HeLa cell line (ab273851) at 20 µg

Lane 3:

Raji at 20 µg

Lane 4:

Recombinant Human PIN1 Protein <a href='/en-us/products/proteins-peptides/recombinant-human-pin1-protein-his-tag-ecoli-ab313358'>ab313358</a> at 0.2 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 18 kDa

Observed band size: 18 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1479Y

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p><strong>For unpurified use at 1/500</strong></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p><strong>For unpurified use at 1/500</strong></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p><strong>For unpurified use at 1/500</strong></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pin1 also known as Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 is a small isomerase enzyme with a molecular weight of approximately 18 kDa. This protein is unique because of its specificity for phosphorylated serine/threonine-proline bonds. Pin1 catalyzes the cis-trans isomerization of these bonds with high efficiency. Expression of the Pin1 protein occurs widely across various tissues such as the brain heart and liver. It predominantly functions in the cytoplasm and nucleus where it modulates the activity of its target proteins by inducing conformational changes.
Biological function summary

Pin1 is essential in regulating cell cycle progression and cellular signaling. It influences a variety of cellular processes including cell proliferation transcriptional regulation and apoptosis. By altering the conformation of specific phosphorylated proteins Pin1 ensures the precise control of cell cycle checkpoints impacting the stability of many proteins such as cyclin D1 and p53. Despite not being part of a large protein complex Pin1 interacts with numerous other proteins thereby coordinating complex regulatory networks essential for maintaining normal cell function.

Pathways

Pin1 plays an important role in both the Wnt signaling pathway and the MAPK signaling pathway. In the Wnt pathway Pin1 maintains stability and the accumulation of β-catenin which affects transcription of target genes critical for cell proliferation. Within the MAPK pathway Pin1 regulates the activity of proteins like c-Jun and ERK1/2 which are vital for transmitting extracellular signals to cellular responses. Through these pathways Pin1 helps modulate responses to external stimuli and maintains cellular homeostasis by adjusting protein function dynamically in response to changes in phosphorylation status.

Changes in Pin1 activity have been linked to cancer and Alzheimer's disease. In cancer increased Pin1 expression accelerates the degradation of tumor suppressor proteins like p53 contributing to uncontrolled cell proliferation and reduced apoptosis. In Alzheimer's disease Pin1 dysfunction leads to the accumulation of phosphorylated tau protein which forms neurofibrillary tangles and disrupts normal neuronal function. Through these connections Pin1 represents a potential therapeutic target for developing treatments aimed at restoring balance in these pathways and alleviating disease symptoms.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Peptidyl-prolyl cis/trans isomerase (PPIase) that binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr-Pro) motifs (PubMed : 21497122, PubMed : 23623683, PubMed : 29686383). By inducing conformational changes in a subset of phosphorylated proteins, acts as a molecular switch in multiple cellular processes (PubMed : 21497122, PubMed : 22033920, PubMed : 23623683). Displays a preference for acidic residues located N-terminally to the proline bond to be isomerized. Regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Down-regulates kinase activity of BTK (PubMed : 16644721). Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation (PubMed : 15664191). Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner (PubMed : 17828269). Acts as a regulator of JNK cascade by binding to phosphorylated FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation : degradation of FBXW7 leads to subsequent stabilization of JUN (PubMed : 22608923). May facilitate the ubiquitination and proteasomal degradation of RBBP8/CtIP through CUL3/KLHL15 E3 ubiquitin-protein ligase complex, hence favors DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR) (PubMed : 23623683, PubMed : 27561354). Upon IL33-induced lung inflammation, catalyzes cis-trans isomerization of phosphorylated IRAK3/IRAK-M, inducing IRAK3 stabilization, nuclear translocation and expression of pro-inflammatory genes in dendritic cells (PubMed : 29686383).
See full target information PIN1
style
left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Antioxidants & redox signaling 30:1621-1634 PubMed30105917

2018

Adaptor Protein p66Shc: A Link Between Cytosolic and Mitochondrial Dysfunction in the Development of Diabetic Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Manish Mishra,Arul J Duraisamy,Sudarshan Bhattacharjee,Renu A Kowluru

Nature communications 9:1603 PubMed29686383

2018

The IL-33-PIN1-IRAK-M axis is critical for type 2 immunity in IL-33-induced allergic airway inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Morris Nechama,Jeahoo Kwon,Shuo Wei,Adrian Tun Kyi,Robert S Welner,Iddo Z Ben-Dov,Mohamed S Arredouani,John M Asara,Chun-Hau Chen,Cheng-Yu Tsai,Kyle F Nelson,Koichi S Kobayashi,Elliot Israel,Xiao Zhen Zhou,Linda K Nicholson,Kun Ping Lu

Neoplasia (New York, N.Y.) 19:216-225 PubMed28167297

2017

Loss of Pin1 Suppresses Hedgehog-Driven Medulloblastoma Tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Xu,Honglai Zhang,Sung-Soo Park,Sriram Venneti,Rork Kuick,Kimberly Ha,Lowell Evan Michael,Mariarita Santi,Chiyoko Uchida,Takafumi Uchida,Ashok Srinivasan,James M Olson,Andrzej A Dlugosz,Sandra Camelo-Piragua,Jean-François Rual

The Journal of cell biology 214:89-101 PubMed27354375

2016

A Presenilin-2-ARF4 trafficking axis modulates Notch signaling during epidermal differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Ellen J Ezratty,H Amalia Pasolli,Elaine Fuchs

Journal of virology 88:547-58 PubMed24173213

2013

The cellular peptidyl-prolyl cis/trans isomerase Pin1 regulates reactivation of Kaposi's sarcoma-associated herpesvirus from latency.

Applications

IP

Species

Unspecified reactive species

Jonathan Guito,Aileen Gavina,Diana Palmeri,David M Lukac

Molecular and cellular biology 32:4306-22 PubMed22907757

2012

The prolyl isomerase Pin1 targets stem-loop binding protein (SLBP) to dissociate the SLBP-histone mRNA complex linking histone mRNA decay with SLBP ubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Nithya Krishnan,Tukiet T Lam,Andrew Fritz,Donald Rempinski,Kieran O'Loughlin,Hans Minderman,Ronald Berezney,William F Marzluff,Roopa Thapar

The Journal of biological chemistry 287:6969-73 PubMed22184106

2011

Prolyl isomerase Pin1 promotes amyloid precursor protein (APP) turnover by inhibiting glycogen synthase kinase-3β (GSK3β) activity: novel mechanism for Pin1 to protect against Alzheimer disease.

Applications

IP

Species

Human

Suk Ling Ma,Lucia Pastorino,Xiao Zhen Zhou,Kun Ping Lu
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB192036

Anti-Pin1 antibody [EPR18546-317]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Pin1 antibody [EPR18546-317] (AB192036)

  • 5
  • 4
Primary Antibodies

AB189321

Anti-Pin1 antibody - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pin1 antibody - N-terminal (AB189321)

  • 0
  • 0

Complementary Products

Assay Kits

AB209879

Mouse Pin1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse Pin1 ELISA Kit (AB209879)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB157474

Anti-ENO1 + ENO3 antibody [EPR11366(B)]

RabMAb|Recombinant

Western blot - Anti-ENO1 + ENO3 antibody [EPR11366(B)] (AB157474)

  • 0
  • 0
Primary Antibodies

AB249362

Anti-DEK antibody [EPR11034] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-DEK antibody [EPR11034] - BSA and Azide free (AB249362)

  • 0
  • 0
Proteins & Peptides

AB313358

Recombinant Human PIN1 Protein (His tag) (E.Coli)

Mass Spectrometry - Recombinant Human PIN1 Protein (His tag) (E.Coli) (AB313358)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com