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AB317396

Anti-Pin1 antibody [S01-7G8]

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Rabbit Monoclonal PIN1 antibody. Suitable for WB, IHC-P and reacts with Human, Rat, Chinese hamster, Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Pin1 aa 1 to C-terminus.

View Alternative Names

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, Peptidyl-prolyl cis-trans isomerase Pin1, Rotamase Pin1, PPIase Pin1, PIN1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pin1 Antibody [S01-7G8] (AB317396)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pin1 Antibody [S01-7G8] (AB317396)

Immunohistochemistry of Pin1 in paraffin-embedded human breast cancer tissue using ab317396 at dilution 1/50.

Western blot - Anti-Pin1 Antibody [S01-7G8] (AB317396)
  • WB

Supplier Data

Western blot - Anti-Pin1 Antibody [S01-7G8] (AB317396)

All lanes:

Western blot - Anti-Pin1 Antibody [S01-7G8] (ab317396) at 1/1000 dilution

Lane 1:

Jurkat cell lysate

Lane 2:

Rat brain lysate

Lane 3:

C6 cell lysate

Lane 4:

CHO-K1 cell lysate

Lane 5:

HeLa cell lysate

Predicted band size: 18 kDa

false

Western blot - Anti-Pin1 Antibody [S01-7G8] (AB317396)
  • WB

Supplier Data

Western blot - Anti-Pin1 Antibody [S01-7G8] (AB317396)

All lanes:

Western blot - Anti-Pin1 Antibody [S01-7G8] (ab317396)

All lanes:

Mouse spleen

Predicted band size: 18 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

S01-7G8

Isotype

IgG

Carrier free

No

Reacts with

Human, Rat, Chinese hamster, Mouse

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Pin1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9QUR7

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Complementary Products

Assay Kits

AB209879

Mouse Pin1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse Pin1 ELISA Kit (AB209879)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Proteins & Peptides

AB313358

Recombinant Human PIN1 Protein (His tag) (E.Coli)

Mass Spectrometry - Recombinant Human PIN1 Protein (His tag) (E.Coli) (AB313358)

  • 0
  • 0
Primary Antibodies

AB124909

Anti-14-3-3 Theta + Tau antibody [EPR5123]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-14-3-3 Theta + Tau antibody [EPR5123] (AB124909)

  • 0
  • 0
Primary Antibodies

AB205270

Anti-active YAP1 antibody [EPR19812]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-active YAP1 antibody [EPR19812] (AB205270)

  • 5
  • 25
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.4 Preservative: 0.01% Sodium azide Constituents: 55.08% Water, 40% Glycerol (glycerin, glycerine), 0.98% Tris glycine, 0.88% Sodium chloride, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pin1 also known as Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 is a small isomerase enzyme with a molecular weight of approximately 18 kDa. This protein is unique because of its specificity for phosphorylated serine/threonine-proline bonds. Pin1 catalyzes the cis-trans isomerization of these bonds with high efficiency. Expression of the Pin1 protein occurs widely across various tissues such as the brain heart and liver. It predominantly functions in the cytoplasm and nucleus where it modulates the activity of its target proteins by inducing conformational changes.
Biological function summary

Pin1 is essential in regulating cell cycle progression and cellular signaling. It influences a variety of cellular processes including cell proliferation transcriptional regulation and apoptosis. By altering the conformation of specific phosphorylated proteins Pin1 ensures the precise control of cell cycle checkpoints impacting the stability of many proteins such as cyclin D1 and p53. Despite not being part of a large protein complex Pin1 interacts with numerous other proteins thereby coordinating complex regulatory networks essential for maintaining normal cell function.

Pathways

Pin1 plays an important role in both the Wnt signaling pathway and the MAPK signaling pathway. In the Wnt pathway Pin1 maintains stability and the accumulation of β-catenin which affects transcription of target genes critical for cell proliferation. Within the MAPK pathway Pin1 regulates the activity of proteins like c-Jun and ERK1/2 which are vital for transmitting extracellular signals to cellular responses. Through these pathways Pin1 helps modulate responses to external stimuli and maintains cellular homeostasis by adjusting protein function dynamically in response to changes in phosphorylation status.

Changes in Pin1 activity have been linked to cancer and Alzheimer's disease. In cancer increased Pin1 expression accelerates the degradation of tumor suppressor proteins like p53 contributing to uncontrolled cell proliferation and reduced apoptosis. In Alzheimer's disease Pin1 dysfunction leads to the accumulation of phosphorylated tau protein which forms neurofibrillary tangles and disrupts normal neuronal function. Through these connections Pin1 represents a potential therapeutic target for developing treatments aimed at restoring balance in these pathways and alleviating disease symptoms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Peptidyl-prolyl cis/trans isomerase (PPIase) that binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr-Pro) motifs (PubMed : 21497122, PubMed : 23623683, PubMed : 29686383). By inducing conformational changes in a subset of phosphorylated proteins, acts as a molecular switch in multiple cellular processes (PubMed : 21497122, PubMed : 22033920, PubMed : 23623683). Displays a preference for acidic residues located N-terminally to the proline bond to be isomerized. Regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Down-regulates kinase activity of BTK (PubMed : 16644721). Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation (PubMed : 15664191). Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner (PubMed : 17828269). Acts as a regulator of JNK cascade by binding to phosphorylated FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation : degradation of FBXW7 leads to subsequent stabilization of JUN (PubMed : 22608923). May facilitate the ubiquitination and proteasomal degradation of RBBP8/CtIP through CUL3/KLHL15 E3 ubiquitin-protein ligase complex, hence favors DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR) (PubMed : 23623683, PubMed : 27561354). Upon IL33-induced lung inflammation, catalyzes cis-trans isomerization of phosphorylated IRAK3/IRAK-M, inducing IRAK3 stabilization, nuclear translocation and expression of pro-inflammatory genes in dendritic cells (PubMed : 29686383). Catalyzes cis-trans isomerization of phosphorylated phosphoglycerate kinase PGK1 under hypoxic conditions to promote its binding to the TOM complex and targeting to the mitochondrion (PubMed : 26942675). Acts as a negative regulator of adipocyte browning by binding to phosphorylated PRDM16, targeting PRDM16 for degradation (By similarity).
See full target information PIN1

Additional targets

Pin1,Pin1
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Product promise

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