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AB155283

Anti-PIN4 antibody [EPR10033]

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(5 Publications)

Rabbit Recombinant Monoclonal PIN4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 5 publications.

View Alternative Names

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4, Parvulin-14, Parvulin-17, Peptidyl-prolyl cis-trans isomerase Pin4, Peptidyl-prolyl cis/trans isomerase EPVH, Rotamase Pin4, Par14, hPar14, Par17, hPar17, PPIase Pin4, hEPVH, PIN4

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-PIN4 antibody [EPR10033] (AB155283)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PIN4 antibody [EPR10033] (AB155283)

Immunocytochemistry/ Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling PIN4 with purified ab155283 at 1/150 dilution (10 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/mL) was used as the secondary antibody only control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] (AB155283)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] (AB155283)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue, labeling PIN4 with ab155283 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] (AB155283)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIN4 antibody [EPR10033] (AB155283)

Immunohistochemical analysis of paraffin embedded Human breast tissue, labeling PIN4 with ab155283 at a 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-PIN4 antibody [EPR10033] (AB155283)
  • WB

Unknown

Western blot - Anti-PIN4 antibody [EPR10033] (AB155283)

All lanes:

Western blot - Anti-PIN4 antibody [EPR10033] (ab155283) at 1/1000 dilution

Lane 1:

293T lysate at 10 µg

Lane 2:

Human skeletal muscle lysate at 10 µg

Lane 3:

Human fetal liver lysate at 10 µg

Lane 4:

Mouse liver lysate at 10 µg

Lane 5:

HepG2 lysate at 10 µg

Lane 6:

RAW264.7 lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 13 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10033

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/150", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Peptidyl-prolyl cis-trans isomerase NIMA-interacting 4 (PIN4) also known as parvulin 14 or essential nuclear protein (Ess1) is an enzyme with a molecular mass of approximately 14 kDa. PIN4 is a member of the parvulin family of peptidyl-prolyl isomerases (PPIs) and facilitates protein folding by catalyzing the cis-trans isomerization of peptide bonds at proline residues. This protein is expressed in a variety of tissues with high expression in the testis and the brain indicating its importance in these tissues.
Biological function summary

PIN4 is involved in several critical cellular processes such as cell cycle regulation and RNA processing. It forms part of a complex with other proteins that influence cell cycle progression and check points. Its role in these processes points to its functional versatility in maintaining cellular homeostasis and modulating interactions with other proteins necessary for DNA synthesis and repair.

Pathways

PIN4 interacts with key signaling cascades such as the cell cycle pathway and the ubiquitin proteasome pathway. In the cell cycle pathway it influences progression through the modulation of phosphorylation states of its substrates. In the ubiquitin proteasome pathway PIN4 impacts protein degradation and related proteins include cyclin-dependent kinases (CDKs) and ubiquitin ligases which together regulate cell division and proteostasis.

PIN4's dysfunction has connections to neurodegenerative disorders and cancer. In neurodegenerative conditions like Alzheimer's disease altered PIN4 activity affects proteins such as tau which plays a role in neuronal stability. In cancer particularly in testicular germ cell tumors PIN4 may partner with proteins like p53 influencing tumor suppression and cell cycle arrest. Understanding these relationships provides insight into potential therapeutic targets and diagnostic markers.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform 1 is involved as a ribosomal RNA processing factor in ribosome biogenesis. Binds to tightly bent AT-rich stretches of double-stranded DNA.. Isoform 2 binds to double-stranded DNA.
See full target information PIN4
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular and infection microbiology 13:1195063 PubMed37404723

2023

Peptidyl-prolyl cis/trans isomerase Pin1 interacts with hepatitis B virus core particle, but not with HBc protein, to promote HBV replication.

Applications

Unspecified application

Species

Unspecified reactive species

Hyeonjoong Kwon,Jumi Kim,Chanho Song,Muhammad Azhar Sajjad,Jiseon Ha,Jaesung Jung,Sun Park,Ho-Joon Shin,Kyongmin Kim

Frontiers in microbiology 14:921653 PubMed36760500

2023

PIN1 and PIN4 inhibition parvulin impeders Juglone, PiB, ATRA, 6,7,4'-THIF, KPT6566, and EGCG thwarted hepatitis B virus replication.

Applications

Unspecified application

Species

Unspecified reactive species

Umar Saeed,Zahra Zahid Piracha

Frontiers in microbiology 12:795047 PubMed34970249

2021

The HBV Core Protein and Core Particle Both Bind to the PPiase Par14 and Par17 to Enhance Their Stabilities and HBV Replication.

Applications

Unspecified application

Species

Unspecified reactive species

Umar Saeed,Zahra Zahid Piracha,Hyeonjoong Kwon,Jumi Kim,Fadia Kalsoom,Yong-Joon Chwae,Sun Park,Ho-Joon Shin,Hyun Woong Lee,Jin Hong Lim,Kyongmin Kim

Journal of virology 93: PubMed30567987

2019

Parvulin 14 and Parvulin 17 Bind to HBx and cccDNA and Upregulate Hepatitis B Virus Replication from cccDNA to Virion in an HBx-Dependent Manner.

Applications

Unspecified application

Species

Unspecified reactive species

Umar Saeed,Jumi Kim,Zahra Zahid Piracha,Hyeonjoong Kwon,Jaesung Jung,Yong-Joon Chwae,Sun Park,Ho-Joon Shin,Kyongmin Kim

Nature 553:222-227 PubMed29323298

2018

A metabolic function of FGFR3-TACC3 gene fusions in cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Véronique Frattini,Stefano M Pagnotta, Tala,Jerry J Fan,Marco V Russo,Sang Bae Lee,Luciano Garofano,Jing Zhang,Peiguo Shi,Genevieve Lewis,Heloise Sanson,Vanessa Frederick,Angelica M Castano,Luigi Cerulo,Delphine C M Rolland,Raghvendra Mall,Karima Mokhtari,Kojo S J Elenitoba-Johnson,Marc Sanson,Xi Huang,Michele Ceccarelli,Anna Lasorella,Antonio Iavarone
View all publications
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Product promise

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