Rabbit Polyclonal PINCH 1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human LIMS1 aa 1-250.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human LIMS1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P48059
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Chicken | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Zebrafish, Xenopus tropicalis | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Zebrafish, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Within the IPP (ILK-PINCH-PARVIN) complex, binds to F-actin, promoting F-actin bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration.
Alternative names
PINCH, PINCH1, LIMS1, LIM and senescent cell antigen-like-containing domain protein 1, Particularly interesting new Cys-His protein 1, Renal carcinoma antigen NY-REN-48, PINCH-1
Recommended products
Rabbit Polyclonal PINCH 1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human LIMS1 aa 1-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human LIMS1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information. Database link P48059
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PINCH 1 also known as LIMS1 is a protein with a molecular mass of approximately 35 kDa. It serves an important role in cellular adhesion and signaling as part of the focal adhesion complex. Expressed widely in many tissues PINCH 1 interacts with integrin-linked kinase (ILK) and parvin proteins to mediate interactions between the extracellular matrix and the actin cytoskeleton. This interaction supports cell adhesion migration and mechanical stability critical for many cellular processes.
- Biological function summary
PINCH 1 facilitates communication within cells by anchoring the cell's structural components to its signaling pathways. It forms the ternary PINCH-ILK-parvin (PIP) complex essential for transmitting mechanical and chemical signals within the cell. This complex enables cells to respond to their environments adjusting their activities accordingly. PINCH 1 also participates in the regulation of cell survival and proliferation suggesting its importance in maintaining cellular functions.
- Pathways
PINCH 1 acts as a participant in the integrin signaling and focal adhesion pathways. In these pathways PINCH 1 works alongside proteins like ILK and alpha-parvin to translate extracellular matrix signals into intracellular responses. These pathways regulate cell movement shape changes and survival by coordinating actin cytoskeleton remodeling and integrin-mediated adhesion. This coordination allows cells to adjust their behavior based on external cues.
- Associated diseases and disorders
PINCH 1 has associations with cancer and cardiovascular diseases where its regulatory role in cell adhesion and signaling becomes evident. Abnormal PINCH 1 expression or function may lead to impaired cell adhesion and increased cell motility contributing to cancer metastasis. In cardiovascular diseases disruptions in PINCH 1 interactions might affect heart tissues' biomechanical integrity through pathways involving ILK. Proper PINCH 1 activity is critical for maintaining the stability and function of heart and tissue cells.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
Western blot - Anti-PINCH 1 antibody (ab154331)
10% SDS PAGE
All lanes: Western blot - Anti-PINCH 1 antibody (ab154331) at 1/1000 dilution
All lanes: HeLa whole cell lysate at 30 µg
Predicted band size: 37 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PINCH 1 antibody (ab154331)
Immunohistochemical analysis of paraffin-embedded BT474 xenograft, labeling PINCH 1 with ab154331 at 1/100 dilution.
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