AB76112

Anti-PINCH 1 + PINCH 2 antibody [EP1943Y]

RabMAb
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(2 Publications)

Rabbit Monoclonal PINCH 1 antibody. Suitable for WB and reacts with Recombinant full length protein - Human, Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

PINCH, PINCH1, LIMS1, LIM and senescent cell antigen-like-containing domain protein 1, Particularly interesting new Cys-His protein 1, Renal carcinoma antigen NY-REN-48, PINCH-1, PINCH2, LIMS2, LIM and senescent cell antigen-like-containing domain protein 2, LIM-like protein 2, Particularly interesting new Cys-His protein 2, PINCH-2

4 Images

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Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (AB76112)

All lanes:

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112) at 1/5000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

293 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 37 kDa

Observed band size: 34-37 kDa

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Supplier Data

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (AB76112)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112) at 1/5000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole lysate at 15 µg

Lane 2:

Mouse kidney lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 34 kDa

false

Supplier Data

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (AB76112)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole lysate at 20 µg

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) whole lysate at 20 µg

Lane 3:

Rat kidney lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 34 kDa

false

Supplier Data

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (AB76112)

Blocking buffer and concentration : 5% NFDM/TBST. Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (ab76112) at 1/1000 dilution

Lane 1:

His-tagged Human full length recombinant LIMS1 protein at 20 ng

Lane 2:

His-tagged Human full length recombinant LIMS2 protein at 20 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 34 and 37 kDa

false

Exposure time: 3s

Carrier free

Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1943Y

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 73% homology with PINCH 2. Cross-reactivity with this protein has not been confirmed experimentally.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

This product has switched from a hybridoma to recombinant production method on 7th September 2023.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PINCH 1 and PINCH 2 also referred to as LIMS1 and LIMS2 are proteins involved in cellular signaling and structural functions. These proteins each have a mass of approximately 37 kDa. They are expressed ubiquitously in various tissues including the heart kidney and smooth muscle among others. Mechanically both PINCH proteins interact with integrin-linked kinase (ILK) and parvin to form the ILK-PINCH-parvin (IPP) complex which plays a significant role in integrin signaling pathways.

Biological function summary

The proteins participate in maintaining cellular adhesion and motility. As members of the IPP complex PINCH 1 and 2 are critical for connecting the extracellular matrix with the actin cytoskeleton. They help transmit signals from the extracellular environment to the cell’s interior influencing cell shape and movement. These proteins are particularly important in the processes of cell survival and tissue development.

Pathways

PINCH proteins engage in integrin-mediated signaling pathways fundamental for cellular responses to mechanical and chemical stimuli. The IPP complex has significant roles in the integrin- and growth factor receptor-mediated pathways especially impacting cell migration and survival. Both proteins link with ILK and through these pathways they interact with various small GTPases that regulate cytoskeletal dynamics.

PINCH 1 and 2 show connections with cancer progression and organ fibrosis. Their expression levels often change in some cancers impacting cell migration and invasion. They also link to fibrosis by influencing extracellular matrix remodeling. Through diseases like cancer PINCH proteins interact with other molecules such as focal adhesion kinase which contributes to altered cell signaling and structure observed in tumor cells and fibrotic tissues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Within the IPP (ILK-PINCH-PARVIN) complex, binds to F-actin, promoting F-actin bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration.

See full target information LIMS1

Additional targets

LIMS2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

STAR protocols 6:103945 PubMed40700012

2025

Protocol for CRISPR-based manipulation and visualization of endogenous α-synuclein in cultured mouse hippocampal neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Leonardo A Parra-Rivas,Rohan Sharma,Trinity E Rust,Hannah O Bazick,Jared Carlson-Stevermer,Mark J Zylka,Yuki Ogawa,Subhojit Roy

The Journal of physiology : PubMed32969494

2020

Integrin-associated ILK and PINCH1 protein content are reduced in skeletal muscle of maintenance hemodialysis patients.

Applications

Unspecified application

Species

Unspecified reactive species

Fulvia Draicchio,Stephan van Vliet,Oana Ancu,Scott A Paluska,Kenneth R Wilund,Monika Mickute,Thozhukat Sathyapalan,Derek Renshaw,Peter Watt,Lykke Sylow,Nicholas A Burd,Richard Mackenzie

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PINCH 1 + PINCH 2 antibody [EP1943Y]

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (AB76112)

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (AB76112)

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (AB76112)

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (AB76112)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (2)

Product promise