JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB284740

Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal PINCH 1 antibody. Carrier free. Suitable for WB and reacts with Recombinant full length protein - Human, Human, Mouse, Rat samples.

View Alternative Names

PINCH, PINCH1, LIMS1, LIM and senescent cell antigen-like-containing domain protein 1, Particularly interesting new Cys-His protein 1, Renal carcinoma antigen NY-REN-48, PINCH-1, PINCH2, LIMS2, LIM and senescent cell antigen-like-containing domain protein 2, LIM-like protein 2, Particularly interesting new Cys-His protein 2, PINCH-2

4 Images
Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] - BSA and Azide free (AB284740)
  • WB

Unknown

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] - BSA and Azide free (AB284740)

This data was developed using ab76112, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (<a href='/en-us/products/primary-antibodies/pinch-1-pinch-2-antibody-ep1943y-ab76112'>ab76112</a>) at 1/5000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

293 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 37 kDa

Observed band size: 34-37 kDa

false

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] - BSA and Azide free (AB284740)
  • WB

Supplier Data

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] - BSA and Azide free (AB284740)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (<a href='/en-us/products/primary-antibodies/pinch-1-pinch-2-antibody-ep1943y-ab76112'>ab76112</a>) at 1/5000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole lysate at 15 µg

Lane 2:

Mouse kidney lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 34 kDa

false

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] - BSA and Azide free (AB284740)
  • WB

Supplier Data

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] - BSA and Azide free (AB284740)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (<a href='/en-us/products/primary-antibodies/pinch-1-pinch-2-antibody-ep1943y-ab76112'>ab76112</a>) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole lysate at 20 µg

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) whole lysate at 20 µg

Lane 3:

Rat kidney lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 34 kDa

false

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] - BSA and Azide free (AB284740)
  • WB

Supplier Data

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] - BSA and Azide free (AB284740)

Blocking buffer and concentration :  5% NFDM/TBST.  Diluting buffer and concentration :  5% NFDM/TBST. This data was developed using ab76112, the same antibody clone in a different buffer formulation

All lanes:

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1943Y] (<a href='/en-us/products/primary-antibodies/pinch-1-pinch-2-antibody-ep1943y-ab76112'>ab76112</a>) at 1/1000 dilution

Lane 1:

His-tagged Human full length recombinant LIMS1 protein at 20 ng

Lane 2:

His-tagged Human full length recombinant LIMS2 protein at 20 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 34 and 37 kDa

false

Exposure time: 3s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1943Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen used for this product shares 73% homology with PINCH 2. Cross-reactivity with this protein has not been confirmed experimentally.

style
left-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB92536

Anti-MMP2 antibody [EPR1184]

20ul selling size|RabMAb|Recombinant

Western blot - Anti-MMP2 antibody [EPR1184] (AB92536)

  • 4
  • 6
Primary Antibodies

AB32360

Anti-NFkB p105 / p50 antibody [E381]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NFkB p105 / p50 antibody [E381] (AB32360)

  • 4
  • 21
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

ab284740 is the carrier-free version of ab76112

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PINCH 1 and PINCH 2 also referred to as LIMS1 and LIMS2 are proteins involved in cellular signaling and structural functions. These proteins each have a mass of approximately 37 kDa. They are expressed ubiquitously in various tissues including the heart kidney and smooth muscle among others. Mechanically both PINCH proteins interact with integrin-linked kinase (ILK) and parvin to form the ILK-PINCH-parvin (IPP) complex which plays a significant role in integrin signaling pathways.
Biological function summary

The proteins participate in maintaining cellular adhesion and motility. As members of the IPP complex PINCH 1 and 2 are critical for connecting the extracellular matrix with the actin cytoskeleton. They help transmit signals from the extracellular environment to the cell’s interior influencing cell shape and movement. These proteins are particularly important in the processes of cell survival and tissue development.

Pathways

PINCH proteins engage in integrin-mediated signaling pathways fundamental for cellular responses to mechanical and chemical stimuli. The IPP complex has significant roles in the integrin- and growth factor receptor-mediated pathways especially impacting cell migration and survival. Both proteins link with ILK and through these pathways they interact with various small GTPases that regulate cytoskeletal dynamics.

PINCH 1 and 2 show connections with cancer progression and organ fibrosis. Their expression levels often change in some cancers impacting cell migration and invasion. They also link to fibrosis by influencing extracellular matrix remodeling. Through diseases like cancer PINCH proteins interact with other molecules such as focal adhesion kinase which contributes to altered cell signaling and structure observed in tumor cells and fibrotic tissues.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Within the IPP (ILK-PINCH-PARVIN) complex, binds to F-actin, promoting F-actin bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration.
See full target information LIMS1

Additional targets

LIMS2
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com