Anti-PINCH 1 + PINCH 2 antibody [EP1944]

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (AB108609)

293T cells were fixed with 100µl 4% PFA for 10 min at RT, then permeabilized with 100µl 90% methanol for 30 min (-20° C) followed by blocking with 10% goat serum for 1h at room temperature. Incubate ab108609 for 30 min at room temperature (RT) and then incubate Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) for 30 min at room temperature.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (AB108609)

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling PINCH 1 + PINCH 2 with ab108609 at 1/1000 dilution. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (AB108609)

Immunofluorescent staining of HeLa cells using ab108609 at 1/100 dilution.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (AB108609)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling ALDH7A1 with ab108609 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution at RT for 45 min. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889) at 1/200 dilution, was co-incubated with ab53278 overnight at 4° C. Nucleus were visualized using DAPI.

• WB

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Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (AB108609)

All lanes:

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (ab108609) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

Human fetal kidney lysate at 10 µg

Lane 5:

Human spleen lysate at 10 µg

Observed band size: 35 kDa

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• WB

Supplier Data

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (AB108609)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (ab108609) at 1/10000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

Mouse kidney lysate at 15 µg

Lane 3:

Rat kidney lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 35 kDa

false

• WB

Supplier Data

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (AB108609)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (ab108609) at 1/10000 dilution

Lane 1:

His-tagged human recombinant LIMS1 protein(aa1-325) at 10 ng

Lane 2:

His-tagged human recombinant LIMS2 protein(aa1-341) at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 35 kDa

false