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Rabbit Recombinant Monoclonal PINCH 1 antibody. Suitable for IHC-P, Flow Cyt (Intra), WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

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Images

Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (AB108609), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (AB108609), expandable thumbnail
  • Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (AB108609), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (AB108609), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (AB108609), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow Cyt (Intra)WBICC/IF
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Expected
Rat
Expected
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10000
Notes

-

Species
Mouse
Dilution info
1/10000
Notes

-

Species
Rat
Dilution info
1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Within the IPP (ILK-PINCH-PARVIN) complex, binds to F-actin, promoting F-actin bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration.

Additional Targets

LIMS2

Alternative names

PINCH, PINCH1, LIMS1, LIM and senescent cell antigen-like-containing domain protein 1, Particularly interesting new Cys-His protein 1, Renal carcinoma antigen NY-REN-48, PINCH-1

Recommended products

Rabbit Recombinant Monoclonal PINCH 1 antibody. Suitable for IHC-P, Flow Cyt (Intra), WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP1944
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

This product has switched from a hybridoma to recombinant production method on 7th September 2023.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PINCH 1 and PINCH 2 also referred to as LIMS1 and LIMS2 are proteins involved in cellular signaling and structural functions. These proteins each have a mass of approximately 37 kDa. They are expressed ubiquitously in various tissues including the heart kidney and smooth muscle among others. Mechanically both PINCH proteins interact with integrin-linked kinase (ILK) and parvin to form the ILK-PINCH-parvin (IPP) complex which plays a significant role in integrin signaling pathways.

Biological function summary

The proteins participate in maintaining cellular adhesion and motility. As members of the IPP complex PINCH 1 and 2 are critical for connecting the extracellular matrix with the actin cytoskeleton. They help transmit signals from the extracellular environment to the cell’s interior influencing cell shape and movement. These proteins are particularly important in the processes of cell survival and tissue development.

Pathways

PINCH proteins engage in integrin-mediated signaling pathways fundamental for cellular responses to mechanical and chemical stimuli. The IPP complex has significant roles in the integrin- and growth factor receptor-mediated pathways especially impacting cell migration and survival. Both proteins link with ILK and through these pathways they interact with various small GTPases that regulate cytoskeletal dynamics.

Associated diseases and disorders

PINCH 1 and 2 show connections with cancer progression and organ fibrosis. Their expression levels often change in some cancers impacting cell migration and invasion. They also link to fibrosis by influencing extracellular matrix remodeling. Through diseases like cancer PINCH proteins interact with other molecules such as focal adhesion kinase which contributes to altered cell signaling and structure observed in tumor cells and fibrotic tissues.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (ab108609), expandable thumbnail

    Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (ab108609)

    All lanes: Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (ab108609) at 1/1000 dilution

    Lane 1: 293T cell lysate at 10 µg

    Lane 2: HeLa cell lysate at 10 µg

    Lane 3: Jurkat cell lysate at 10 µg

    Lane 4: Human fetal kidney lysate at 10 µg

    Lane 5: Human spleen lysate at 10 µg

    Observed band size: 35 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (ab108609), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (ab108609)

    Immunofluorescent staining of HeLa cells using ab108609 at 1/100 dilution.

  • Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (ab108609), expandable thumbnail

    Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (ab108609)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (ab108609) at 1/10000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Lane 2: Mouse kidney lysate at 15 µg

    Lane 3: Rat kidney lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 35 kDa

  • Flow Cytometry (Intracellular) - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (ab108609), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (ab108609)

    293T cells were fixed with 100?l 4% PFA for 10 min at RT, then permeabilized with 100?l 90% methanol for 30 min (-20° C) followed by blocking with 10% goat serum for 1h at room temperature. Incubate ab108609 for 30 min at room temperature (RT) and then incubate Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) for 30 min at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (ab108609), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (ab108609)

    Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling PINCH 1 + PINCH 2 with ab108609 at 1/1000 dilution. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunocytochemistry/ Immunofluorescence - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (ab108609), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (ab108609)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling ALDH7A1 with ab108609 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/1000 dilution at RT for 45 min. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution, was co-incubated with Anti-ALDH7A1 antibody [EP1935Y] ab53278 overnight at 4° C. Nucleus were visualized using DAPI.

  • Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (ab108609), expandable thumbnail

    Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (ab108609)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PINCH 1 + PINCH 2 antibody [EP1944] (ab108609) at 1/10000 dilution

    Lane 1: His-tagged human recombinant LIMS1 protein(aa1-325) at 10 ng

    Lane 2: His-tagged human recombinant LIMS2 protein(aa1-341) at 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 35 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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