Rabbit Recombinant Monoclonal PINK1 antibody. Carrier free. Suitable for I-ELISA, ICC/IF, WB and reacts with Recombinant fragment - Human, Human samples.
Images
![Western blot - Anti-PINK1 antibody [EPR29146-340] - BSA and Azide free (AB324111), expandable thumbnail](https://content.abcam.com/products/images/pink1-antibody-epr29146-340-bsa-and-azide-free-ab324111--western-blot-img445580.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PINK1 antibody [EPR29146-340] - BSA and Azide free (AB324111)")
![Western blot - Anti-PINK1 antibody [EPR29146-340] - BSA and Azide free (AB324111), expandable thumbnail](https://content.abcam.com/products/images/pink1-antibody-epr29146-340-bsa-and-azide-free-ab324111--western-blot-img445579.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PINK1 antibody [EPR29146-340] - BSA and Azide free (AB324111)")
![Immunocytochemistry/ Immunofluorescence - Anti-PINK1 antibody [EPR29146-340] - BSA and Azide free (AB324111), expandable thumbnail](https://content.abcam.com/products/images/pink1-antibody-epr29146-340-bsa-and-azide-free-ab324111--immunocytochemistry-immunofluorescence-img445571.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PINK1 antibody [EPR29146-340] - BSA and Azide free (AB324111)")
![Immunocytochemistry/ Immunofluorescence - Anti-PINK1 antibody [EPR29146-340] - BSA and Azide free (AB324111), expandable thumbnail](https://content.abcam.com/products/images/pink1-antibody-epr29146-340-bsa-and-azide-free-ab324111--immunocytochemistry-immunofluorescence-img445570.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-PINK1 antibody [EPR29146-340] - BSA and Azide free (AB324111)")
![Indirect ELISA - Anti-PINK1 antibody [EPR29146-340] - BSA and Azide free (AB324111), expandable thumbnail](https://content.abcam.com/products/images/pink1-antibody-epr29146-340-bsa-and-azide-free-ab324111--indirect-elisa-img445518.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-PINK1 antibody [EPR29146-340] - BSA and Azide free (AB324111)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| I-ELISA | ICC/IF | WB | IHC-P | |
|---|---|---|---|---|
| Human | Expected | Tested | Tested | Not recommended |
| Recombinant fragment - Human | Tested | Not recommended | Not recommended | Not recommended |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Recombinant fragment - Human | Dilution info - | Notes - |
Target data
Function
Serine/threonine-protein kinase which acts as a sensor of mitochondrial damage and protects against mitochondrial dysfunction during cellular stress. It phosphorylates mitochondrial proteins to coordinate mitochondrial quality control mechanisms that remove and replace dysfunctional mitochondrial components (PubMed:14607334, PubMed:15087508, PubMed:18443288, PubMed:18957282, PubMed:19229105, PubMed:19966284, PubMed:20404107, PubMed:20547144, PubMed:20798600, PubMed:22396657, PubMed:23620051, PubMed:23754282, PubMed:23933751, PubMed:24660806, PubMed:24751536, PubMed:24784582, PubMed:24896179, PubMed:24898855, PubMed:25527291, PubMed:32484300). Depending on the severity of mitochondrial damage, activity ranges from preventing apoptosis and stimulating mitochondrial biogenesis to eliminating severely damaged mitochondria via PINK1-PRKN-dependent mitophagy (PubMed:14607334, PubMed:15087508, PubMed:18443288, PubMed:19966284, PubMed:20404107, PubMed:20798600, PubMed:22396657, PubMed:23620051, PubMed:23933751, PubMed:24898855, PubMed:32047033, PubMed:32484300). When cellular stress results in irreversible mitochondrial damage, PINK1 accumulates at the outer mitochondrial membrane (OMM) where it phosphorylates pre-existing polyubiquitin chains at 'Ser-65', recruits PRKN from the cytosol to the OMM and activates PRKN by phosphorylation at 'Ser-65'; activated PRKN then ubiquinates VDAC1 and other OMM proteins to initiate mitophagy (PubMed:14607334, PubMed:15087508, PubMed:19966284, PubMed:20404107, PubMed:20798600, PubMed:23754282, PubMed:23933751, PubMed:24660806, PubMed:24751536, PubMed:24784582, PubMed:25474007, PubMed:25527291, PubMed:32047033). The PINK1-PRKN pathway also promotes fission of damaged mitochondria through phosphorylation and PRKN-dependent degradation of mitochondrial proteins involved in fission such as MFN2 (PubMed:18443288, PubMed:23620051, PubMed:24898855). This prevents the refusion of unhealthy mitochondria with the mitochondrial network or initiates mitochondrial fragmentation facilitating their later engulfment by autophagosomes (PubMed:18443288, PubMed:23620051). Also promotes mitochondrial fission independently of PRKN and ATG7-mediated mitophagy, via the phosphorylation and activation of DNM1L (PubMed:18443288, PubMed:32484300). Regulates motility of damaged mitochondria by promoting the ubiquitination and subsequent degradation of MIRO1 and MIRO2; in motor neurons, this likely inhibits mitochondrial intracellular anterograde transport along the axons which probably increases the chance of the mitochondria undergoing mitophagy in the soma (PubMed:22396657). Required for ubiquinone reduction by mitochondrial complex I by mediating phosphorylation of complex I subunit NDUFA10 (By similarity). Phosphorylates LETM1, positively regulating its mitochondrial calcium transport activity (PubMed:29123128).
Alternative names
BRPK, PTEN-induced putative kinase protein 1, PINK1
Rabbit Recombinant Monoclonal PINK1 antibody. Carrier free. Suitable for I-ELISA, ICC/IF, WB and reacts with Recombinant fragment - Human, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR29146-340
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab324111 is the carrier free version of Anti-PINK1 antibody [EPR29146-340] ab323807.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-PINK1 antibody [EPR29146-340] - BSA and Azide free (ab324111), expandable thumbnail](https://content.abcam.com/products/images/pink1-antibody-epr29146-340-bsa-and-azide-free-ab324111--western-blot-img445580.jpg "Western blot - Anti-PINK1 antibody [EPR29146-340] - BSA and Azide free (ab324111)")
Western blot - Anti-PINK1 antibody [EPR29146-340] - BSA and Azide free (ab324111)
PINK1 Western blot staining using rabbit Anti-PINK1 antibody
This data was developed using Anti-PINK1 antibody [EPR29146-340] ab323807, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Performed under reducing conditions.
In Western blot Anti-PINK1 antibody [EPR29146-340] ab323807 was shown to bind specifically to PINK1. Target of interest was observed at 62 kDa in wild-type HEK-293 cell lysates (lane 2) with no signal observed at this size in PINK1 knockout cell line (lane 4) (lane 4 knockout cell line Human PINK1 knockout HEK-293T cell line ab266393).
The identity of the higher MW band at approximately 250 kDa (in lanes 1-4) is unknown.
The expression of PINK1 is upregulated in response to CCCP treatment (PMID:22724072).
In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-PINK1 antibody [EPR29146-340] (Anti-PINK1 antibody [EPR29146-340] ab323807) at 1/1000 dilution
Lane 1: Untreated wild-type HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 80 µg
Lane 2: Wild-type HEK-293 treated with 30μM CCCP for 6h, whole cell lysate at 80 µg
Lane 3: Untreated PINK1 knockout HEK-293 whole cell lysate at 80 µg
Lane 4: PINK1 knockout HEK-293 treated with 30μM CCCP for 6h, whole cell lysate at 80 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 62 kDa, 36 kDa
Exposure time: 125s
![Western blot - Anti-PINK1 antibody [EPR29146-340] - BSA and Azide free (ab324111), expandable thumbnail](https://content.abcam.com/products/images/pink1-antibody-epr29146-340-bsa-and-azide-free-ab324111--western-blot-img445579.jpg "Western blot - Anti-PINK1 antibody [EPR29146-340] - BSA and Azide free (ab324111)")
Western blot - Anti-PINK1 antibody [EPR29146-340] - BSA and Azide free (ab324111)
PINK1 Western blot staining using rabbit Anti-PINK1 antibody
This data was developed using Anti-PINK1 antibody [EPR29146-340] ab323807, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Performed under reducing conditions.
In Western blot Anti-PINK1 antibody [EPR29146-340] ab323807 was shown to bind specifically to PINK1. Target of interest was observed at 62 kDa in wild-type HEK-293 cell lysates (lane 2) with no signal observed at this size in PINK1 knockout cell line (lane 4) (lane 4 knockout cell line Human PINK1 knockout HEK-293T cell line ab266393).
The identity of the higher MW band at approximately 250 kDa (in lanes 1-4) is unknown.
The expression of PINK1 is upregulated in response to valinomycin treatment (PMID:22724072).
The expression of PINK1 is upregulated in response to CCCP treatment (PMID:22724072).
In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lanes 1-4: 48 seconds Lanes 5-6: 10 seconds
All lanes: Western blot - Anti-PINK1 antibody [EPR29146-340] (Anti-PINK1 antibody [EPR29146-340] ab323807) at 1/1000 dilution
Lane 1: Untreated wild-type HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 2: Wild-type HEK-293 treated with 10μM valinomycin for 24h, whole cell lysate at 20 µg
Lane 3: Untreated PINK1 knockout HEK-293 whole cell lysate at 20 µg
Lane 4: PINK1 knockout HEK-293 treated with 10μM valinomycin for 24h, whole cell lysate at 20 µg
Lane 5: Untreated SK-OV-3 (human ovarian cancer epithelial cell) whole cell lysate at 20 µg
Lane 6: SK-OV-3 treated with 30μM CCCP for 6h, whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 62 kDa, 36 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-PINK1 antibody [EPR29146-340] - BSA and Azide free (ab324111), expandable thumbnail](https://content.abcam.com/products/images/pink1-antibody-epr29146-340-bsa-and-azide-free-ab324111--immunocytochemistry-immunofluorescence-img445571.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PINK1 antibody [EPR29146-340] - BSA and Azide free (ab324111)")
Immunocytochemistry/ Immunofluorescence - Anti-PINK1 antibody [EPR29146-340] - BSA and Azide free (ab324111)
PINK1 Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-PINK1 antibody
This data was developed using Anti-PINK1 antibody [EPR29146-340] ab323807, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed 0.1% TritonX-100 permeabilized PINK1 KO HEK-293T (PINK1 knockout human embryonic kidney epithelial cell) Human PINK1 knockout HEK-293T cell line ab266393 cells labelling PINK1 with Anti-PINK1 antibody [EPR29146-340] ab323807 at 1/50 (10.34 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 g/ml dilution (Green).
Confocal image showing increased mitochondrial staining in wildtype HEK-293T cells treated with 10 µM Valinomycin for 24 hours (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 g/ml dilution.
anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used to counterstain tubulin at 1/200 2.5 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Counterstain Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 2 g/ml dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-PINK1 antibody [EPR29146-340] - BSA and Azide free (ab324111), expandable thumbnail](https://content.abcam.com/products/images/pink1-antibody-epr29146-340-bsa-and-azide-free-ab324111--immunocytochemistry-immunofluorescence-img445570.jpg "Immunocytochemistry/ Immunofluorescence - Anti-PINK1 antibody [EPR29146-340] - BSA and Azide free (ab324111)")
Immunocytochemistry/ Immunofluorescence - Anti-PINK1 antibody [EPR29146-340] - BSA and Azide free (ab324111)
PINK1 Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-PINK1 antibody
This data was developed using Anti-PINK1 antibody [EPR29146-340] ab323807, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed 0.1% TritonX-100 permeabilized SK-OV-3(human ovarian cancer epithelial cell) cells labelling PINK1 with Anti-PINK1 antibody [EPR29146-340] ab323807 at 1/50 (10.34 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 g/ml dilution (Green).
Confocal image showing mitochondrial staining in SK-OV-3 cells (shown in green) treatment with 30 µM CCCP for 6 hours. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 g/ml dilution.
anti-COX IV mouse monoclonal antibody - Mitochondrial Marker was used to counterstain tubulin at 1/200 2.5 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Counterstain Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 2 g/ml dilution.
![Indirect ELISA - Anti-PINK1 antibody [EPR29146-340] - BSA and Azide free (ab324111), expandable thumbnail](https://content.abcam.com/products/images/pink1-antibody-epr29146-340-bsa-and-azide-free-ab324111--indirect-elisa-img445518.jpg "Indirect ELISA - Anti-PINK1 antibody [EPR29146-340] - BSA and Azide free (ab324111)")
Indirect ELISA - Anti-PINK1 antibody [EPR29146-340] - BSA and Azide free (ab324111)
This data was developed using Anti-PINK1 antibody [EPR29146-340] ab323807, the same antibody clone in a different buffer formulation.
Indirect ELISA analysis of Anti-PINK1 antibody [EPR29146-340] ab323807 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1:2500 dilution dilution.
Antigen: Human PINK1.
Antigen concentration: 1000 ng/ml
"
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com