Rabbit Recombinant Monoclonal PINK1 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-PINK1 antibody [MJF-R32-7] - BSA and Azide free (AB300624), expandable thumbnail](https://content.abcam.com/products/images/pink1-antibody-mjf-r32-7-bsa-and-azide-free-ab300624--western-blot-img410095.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PINK1 antibody [MJF-R32-7] - BSA and Azide free (AB300624)")
![Western blot - Anti-PINK1 antibody [MJF-R32-7] - BSA and Azide free (AB300624), expandable thumbnail](https://content.abcam.com/products/images/pink1-antibody-mjf-r32-7-bsa-and-azide-free-ab300624--western-blot-img426534.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PINK1 antibody [MJF-R32-7] - BSA and Azide free (AB300624)")
![Western blot - Anti-PINK1 antibody [MJF-R32-7] - BSA and Azide free (AB300624), expandable thumbnail](https://content.abcam.com/products/images/pink1-antibody-mjf-r32-7-bsa-and-azide-free-ab300624--western-blot-img401961.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PINK1 antibody [MJF-R32-7] - BSA and Azide free (AB300624)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | ICC/IF | IHC-P | WB | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
Target data
Function
Serine/threonine-protein kinase which acts as a sensor of mitochondrial damage and protects against mitochondrial dysfunction during cellular stress. It phosphorylates mitochondrial proteins to coordinate mitochondrial quality control mechanisms that remove and replace dysfunctional mitochondrial components (PubMed:14607334, PubMed:15087508, PubMed:18443288, PubMed:18957282, PubMed:19229105, PubMed:19966284, PubMed:20404107, PubMed:20547144, PubMed:20798600, PubMed:22396657, PubMed:23620051, PubMed:23754282, PubMed:23933751, PubMed:24660806, PubMed:24751536, PubMed:24784582, PubMed:24896179, PubMed:24898855, PubMed:25527291, PubMed:32484300). Depending on the severity of mitochondrial damage, activity ranges from preventing apoptosis and stimulating mitochondrial biogenesis to eliminating severely damaged mitochondria via PINK1-PRKN-dependent mitophagy (PubMed:14607334, PubMed:15087508, PubMed:18443288, PubMed:19966284, PubMed:20404107, PubMed:20798600, PubMed:22396657, PubMed:23620051, PubMed:23933751, PubMed:24898855, PubMed:32047033, PubMed:32484300). When cellular stress results in irreversible mitochondrial damage, PINK1 accumulates at the outer mitochondrial membrane (OMM) where it phosphorylates pre-existing polyubiquitin chains at 'Ser-65', recruits PRKN from the cytosol to the OMM and activates PRKN by phosphorylation at 'Ser-65'; activated PRKN then ubiquinates VDAC1 and other OMM proteins to initiate mitophagy (PubMed:14607334, PubMed:15087508, PubMed:19966284, PubMed:20404107, PubMed:20798600, PubMed:23754282, PubMed:23933751, PubMed:24660806, PubMed:24751536, PubMed:24784582, PubMed:25474007, PubMed:25527291, PubMed:32047033). The PINK1-PRKN pathway also promotes fission of damaged mitochondria through phosphorylation and PRKN-dependent degradation of mitochondrial proteins involved in fission such as MFN2 (PubMed:18443288, PubMed:23620051, PubMed:24898855). This prevents the refusion of unhealthy mitochondria with the mitochondrial network or initiates mitochondrial fragmentation facilitating their later engulfment by autophagosomes (PubMed:18443288, PubMed:23620051). Also promotes mitochondrial fission independently of PRKN and ATG7-mediated mitophagy, via the phosphorylation and activation of DNM1L (PubMed:18443288, PubMed:32484300). Regulates motility of damaged mitochondria by promoting the ubiquitination and subsequent degradation of MIRO1 and MIRO2; in motor neurons, this likely inhibits mitochondrial intracellular anterograde transport along the axons which probably increases the chance of the mitochondria undergoing mitophagy in the soma (PubMed:22396657). Required for ubiquinone reduction by mitochondrial complex I by mediating phosphorylation of complex I subunit NDUFA10 (By similarity). Phosphorylates LETM1, positively regulating its mitochondrial calcium transport activity (PubMed:29123128).
Alternative names
BRPK, PTEN-induced putative kinase protein 1, PINK1
Recommended products
Rabbit Recombinant Monoclonal PINK1 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- MJF-R32-7
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody does not react in: IHC-P with human, mouse and rat; and in immunocytochemistry, flow cytometry and immunoprecipitation with human.
This antibody was mapped to AA 188-194 with some cross-reaction to AA 287-301.- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Collaborations
This antibody was developed with support from The Michael J. Fox Foundation.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The PTEN-induced kinase 1 (PINK1) is a serine/threonine-protein kinase with a molecular weight of approximately 63 kDa. It plays a significant role in mitochondrial quality control through its kinase activity. PINK1 gets expressed in various tissues with a high presence in the brain. The protein localizes to the outer membrane of damaged mitochondria to initiate mitophagy an important cellular process for clearing dysfunctional mitochondria.
- Biological function summary
The PINK1 protein detects mitochondrial damage and recruits Parkin an E3 ubiquitin ligase to the damaged mitochondria. This interaction leads to the ubiquitylation of mitochondrial substrates and triggers their degradation. PINK1 Parkin and other proteins form a complex that facilitates the removal of damaged mitochondria through autophagy. This mechanism ensures cellular health and energy balance by maintaining a pool of functional mitochondria.
- Pathways
PINK1 integrates into the mitochondrial quality control and mitophagy pathways. It has a fundamental role in the PINK1-Parkin pathway which is critical for maintaining mitochondrial integrity. In this pathway PINK1 phosphorylates both ubiquitin and Parkin enhancing Parkin’s E3 ligase activity. Another pathway that PINK1 participates in is the PTEN-induced kinase pathway where it regulates mitochondrial dynamics and homeostasis via cross-talk with other mitochondrial proteins such as DJ-1 and LRRK2.
- Associated diseases and disorders
PINK1 mutations are strongly associated with familial Parkinson’s disease attributing to its role in preserving neuronal function through mitochondrial regulation. Deficient PINK1 function leads to accumulation of damaged mitochondria contributing to neuronal cell death. Additionally PINK1 dysfunction is implicated in Alzheimer’s disease as impaired mitochondrial clearance is a contributing factor. Here the PINK1 interaction with other proteins like Parkin and DJ-1 highlights its importance in neurodegenerative disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-PINK1 antibody [MJF-R32-7] - BSA and Azide free (ab300624), expandable thumbnail](https://content.abcam.com/products/images/pink1-antibody-mjf-r32-7-bsa-and-azide-free-ab300624--western-blot-img410095.jpg "Western blot - Anti-PINK1 antibody [MJF-R32-7] - BSA and Azide free (ab300624)")
Western blot - Anti-PINK1 antibody [MJF-R32-7] - BSA and Azide free (ab300624)
This data was developed using Anti-PINK1 antibody [MJF-R32-7] ab300623, the same antibody clone in a different buffer formulation.
Anti-PINK1 antibody [MJF-R32-7] (Anti-PINK1 antibody [MJF-R32-7] ab300623) staining at 1/1000 dilution, shown in black; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. Anti-PINK1 antibody [MJF-R32-7] ab300623 was shown to bind specifically to PINK1. A band was observed at 60 kDa in wild-type HEK-293 cell lysates with no signal observed at this size in PINK1 knockout cell line Human PINK1 knockout HEK-293T cell line ab266393 (knockout cell lysate ab257030). To generate this image, wild-type and PINK1 knockout HEK-293 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent Anti-PKC delta (phospho S299) antibody [EPNCI119] ab133456) and imaged with 4 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.All lanes: Western blot - Anti-PINK1 antibody [MJF-R32-7] (Anti-PINK1 antibody [MJF-R32-7] ab300623) at 1/1000 dilution
Lane 1: Wild-type HEK-293 Vehicle Control CCCP, CCCP, Mitochondrial oxidative phosphorylation uncoupler ab141229 (0 µM, 24 h) cell lysate at 20 µg
Lane 2: Wild-type HEK-293 Treated CCCP, CCCP, Mitochondrial oxidative phosphorylation uncoupler ab141229 (10 µM, 24 h) cell lysate at 20 µg
Lane 3: PINK1 knockout HEK-293 Vehicle Control CCCP, CCCP, Mitochondrial oxidative phosphorylation uncoupler ab141229 (0 µM, 24 h) cell lysate at 20 µg
Lanes 3 - 4: Western blot - Human PINK1 knockout HEK-293T cell line (Human PINK1 knockout HEK-293T cell line ab266393)
Lane 4: PINK1 knockout HEK-293 Treated CCCP, CCCP, Mitochondrial oxidative phosphorylation uncoupler ab141229 (10 µM, 24 h) cell lysate at 20 µg
SecondaryAll lanes: HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 63 kDa
![Western blot - Anti-PINK1 antibody [MJF-R32-7] - BSA and Azide free (ab300624), expandable thumbnail](https://content.abcam.com/products/images/pink1-antibody-mjf-r32-7-bsa-and-azide-free-ab300624--western-blot-img426534.jpg "Western blot - Anti-PINK1 antibody [MJF-R32-7] - BSA and Azide free (ab300624)")
Western blot - Anti-PINK1 antibody [MJF-R32-7] - BSA and Azide free (ab300624)
This data was developed using Anti-PINK1 antibody [MJF-R32-7] ab300623, the same antibody clone in a different buffer formulation.
Western blot: Anti-PINK1 antibody [MJF-R32-7] (Anti-PINK1 antibody [MJF-R32-7] ab300623) staining at 1/1000 dilution, shown in black; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta.
In Western blot, Anti-PINK1 antibody [MJF-R32-7] ab300623 was shown to bind specifically to PINK1. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with a high-sensitivity ECL substrate kit and imaged with 20 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
In Western blot, Anti-PINK1 antibody [MJF-R32-7] ab300623 was shown to bind specifically to PINK1. A band was observed at 63kDa in wild-type SH-SY5Y treated with CCCP cell lysates with no signal observed at this size in PINK1 knockout cell line (Human PINK1 knockout SH-SY5Y cell line ab280876).
All lanes: Western blot - Anti-PINK1 antibody [MJF-R32-7] (Anti-PINK1 antibody [MJF-R32-7] ab300623)
Lane 1: Wild-type SH-SY5Y treated CCCP, CCCP, Mitochondrial oxidative phosphorylation uncoupler ab141229 (10 μM, 24 h) cell lysate at 20 µg
Lane 2: Wild-type SH-SY5Y control CCCP (0 μM, 24 h) cell lysate at 20 µg
Lane 3: PINK1 knockout SH-SY5Y (Human PINK1 knockout SH-SY5Y cell line ab280876) treated CCCP, CCCP, Mitochondrial oxidative phosphorylation uncoupler ab141229 (10 uM, 24 h) cell lysate at 20 µg
Lane 4: Western blot - Human PINK1 knockout SH-SY5Y cell line (Human PINK1 knockout SH-SY5Y cell line ab280876) at 20 µg
Lane 5: Wild-type HEK-293 Treated CCCP, CCCP, Mitochondrial oxidative phosphorylation uncoupler ab141229 (10 μM, 24 h) cell lysate at 20 µg
Lane 6: PINK1 knockout HEK-293 Treated CCCP, CCCP, Mitochondrial oxidative phosphorylation uncoupler ab141229 (10 μM, 24 h) cell lysate at 20 µg
SecondaryAll lanes: HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 63 kDa
Observed band size: 63 kDa
![Western blot - Anti-PINK1 antibody [MJF-R32-7] - BSA and Azide free (ab300624), expandable thumbnail](https://content.abcam.com/products/images/pink1-antibody-mjf-r32-7-bsa-and-azide-free-ab300624--western-blot-img401961.jpg "Western blot - Anti-PINK1 antibody [MJF-R32-7] - BSA and Azide free (ab300624)")
Western blot - Anti-PINK1 antibody [MJF-R32-7] - BSA and Azide free (ab300624)
All lanes: Western blot - Anti-PINK1 antibody [MJF-R32-7] (Anti-PINK1 antibody [MJF-R32-7] ab300623) at 1/1000 dilution
All lanes: Rat testis tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com