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AB324681

Anti-PINK1 (phospho S401) antibody [EPR26308-532]

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Rabbit Recombinant Monoclonal PINK1 phospho S401 antibody. Suitable for WB, Dot and reacts with Transfected cell lysate - Mouse, Mouse, Rat, Synthetic peptide samples.

View Alternative Names

BRPK, PTEN-induced putative kinase protein 1

4 Images
Western blot - Anti-PINK1 (phospho S401) antibody [EPR26308-532] (AB324681)
  • WB

Supplier Data

Western blot - Anti-PINK1 (phospho S401) antibody [EPR26308-532] (AB324681)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-PINK1 (phospho S401) antibody [EPR26308-532] (ab324681) at 1/1000 dilution

Lane 1:

Untreated PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate (untreated membrane) at 20 µg

Lane 2:

PC-12 treated with 10uM Valinomycin for 24h whole cell lysate (untreated membrane) at 20 µg

Lane 3:

PC-12 treated with 10uM Valinomycin for 24h whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 63 kDa,36 kDa

true

Exposure time: 81s

Western blot - Anti-PINK1 (phospho S401) antibody [EPR26308-532] (AB324681)
  • WB

Supplier Data

Western blot - Anti-PINK1 (phospho S401) antibody [EPR26308-532] (AB324681)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-PINK1 (phospho S401) antibody [EPR26308-532] (ab324681) at 1/1000 dilution

Lane 1:

Untreated MEF (mouse embryo fibroblast) whole cell lysate (untreated membrane) at 20 µg

Lane 2:

MEF treated with 10uM Valinomycin for 24h whole cell lysate (untreated membrane) at 20 µg

Lane 3:

MEF treated with 10uM Valinomycin for 24h whole cell lysate (alkaline phosphatase treated membrane) at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 63 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-PINK1 (phospho S401) antibody [EPR26308-532] (AB324681)
  • WB

Supplier Data

Western blot - Anti-PINK1 (phospho S401) antibody [EPR26308-532] (AB324681)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-PINK1 (phospho S401) antibody [EPR26308-532] (ab324681) at 1/1000 dilution

Lane 1:

Untreated 293T (human embryonic kidney epithelial cell) cells transfected with a mouse wild-type Pink1 expression vector containing a myc-His-tag®, whole cell lysate  at 20 µg

Lane 2:

293T cells transfected with a mouse wild-type Pink1 expression vector containing a myc-His-tag®, then treated with 10uM Valinomycin for 24h whole cell lysate at 20 µg

Lane 3:

Untreated 293T cells transfected with a mouse Pink1(mutation S401A) expression vector containing a myc-His-tag®, whole cell lysate  at 20 µg

Lane 4:

293T cells transfected with a mouse Pink1(mutation S401A) expression vector containing a myc-His-tag®, then treated with 10uM Valinomycin for 24h whole cell lysate  at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 63 kDa,36 kDa

false

Exposure time: 37s

Dot Blot - Anti-PINK1 (phospho S401) antibody [EPR26308-532] (AB324681)
  • Dot

Supplier Data

Dot Blot - Anti-PINK1 (phospho S401) antibody [EPR26308-532] (AB324681)

Dot blot analysis of PINK1 (phospho S401) using ab324681 at 1 : 1000 (0.493 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : PINK1 (phospho S401) peptide
Lane2 : PINK1 non-phospho peptide

Exposure time : 10 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-PINK1 (phospho S401) antibody [EPR26308-532] (ab324681) at 1/1000 dilution

Lane 1:

PINK1 (phospho S401) peptide

Lane 2:

PINK1 non-phospho peptide

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26308-532

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Synthetic peptide": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Transfected cell lysate - Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase which acts as a sensor of mitochondrial damage and protects against mitochondrial dysfunction during cellular stress. It phosphorylates mitochondrial proteins to coordinate mitochondrial quality control mechanisms that remove and replace dysfunctional mitochondrial components (PubMed : 24652937, PubMed : 24784582, PubMed : 25474007, PubMed : 32484300). Depending on the severity of mitochondrial damage, activity ranges from preventing apoptosis and stimulating mitochondrial biogenesis to eliminating severely damaged mitochondria via PINK1-PRKN-dependent mitophagy (By similarity). When cellular stress results in irreversible mitochondrial damage, PINK1 accumulates at the outer mitochondrial membrane (OMM) where it phosphorylates pre-existing polyubiquitin chains at 'Ser-65', recruits PRKN from the cytosol to the OMM and activates PRKN by phosphorylation at 'Ser-65' (PubMed : 24652937, PubMed : 24784582, PubMed : 25474007, PubMed : 32484300). Activated PRKN then ubiquinates VDAC1 and other OMM proteins to initiate mitophagy (By similarity). The PINK1-PRKN pathway also promotes fission of damaged mitochondria by phosphorylating and thus promoting the PRKN-dependent degradation of mitochondrial proteins involved in fission such as MFN2 (By similarity). This prevents the refusion of unhealthy mitochondria with the mitochondrial network or initiates mitochondrial fragmentation facilitating their later engulfment by autophagosomes (By similarity). Also promotes mitochondrial fission independently of PRKN and ATG7-mediated mitophagy, via the phosphorylation and activation of DNM1L (PubMed : 32484300). Regulates motility of damaged mitochondria by promoting the ubiquitination and subsequent degradation of MIRO1 and MIRO2; in motor neurons, this likely inhibits mitochondrial intracellular anterograde transport along the axons which probably increases the chance of the mitochondria undergoing mitophagy in the soma (By similarity). Required for ubiquinone reduction by mitochondrial complex I by mediating phosphorylation of complex I subunit NDUFA10 (PubMed : 24652937). Phosphorylates LETM1, positively regulating its mitochondrial calcium transport activity (PubMed : 29123128).
See full target information Pink1 phospho S401
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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