AB190252

Anti-PINX1/LPTS antibody

Name: Anti-PINX1/LPTS antibody (ab190252) | Abcam
Brand: Abcam Limited
SKU: AB190252

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(2 Publications)

Rabbit Polyclonal PINX1/LPTS antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PINX1 aa 200-300.

View Alternative Names

LPTL, LPTS, PINX1, PIN2/TERF1-interacting telomerase inhibitor 1, Liver-related putative tumor suppressor, Pin2-interacting protein X1, Protein 67-11-3, TRF1-interacting protein 1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PINX1/LPTS antibody (AB190252)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PINX1/LPTS antibody (AB190252)

Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections using ab190252 at 1/1000 dilution (1 μg/mL).

Supplier Data

Immunoprecipitation - Anti-PINX1/LPTS antibody (AB190252)

Detection of PINX1/LPTS in immunoprecipitates of Jurkat whole cell lysates (1 mg for IP, 20% of IP loaded) using ab190252 at 6 μg/reaction for IP and 1μg/ml for WB detection (Lane 1). Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-PINX1/LPTS antibody (ab190252)

Predicted band size: 37 kDa

false

Supplier Data

Western blot - Anti-PINX1/LPTS antibody (AB190252)

All lanes:

Western blot - Anti-PINX1/LPTS antibody (ab190252) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 37 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, IP

applications

Immunogen

Synthetic Peptide within Human PINX1 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

Q96BK5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "Epitope retrieval with citrate buffer pH 6.0 is recommended for tissue sections." }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab190252 was affinity purified using an epitope specific to PINX1/LPTS immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PINX1 also known as LPTS is a nuclear protein known for its role as a telomerase inhibitor. It has a molecular mass of approximately 42 kDa. This protein is expressed in various tissues including the heart liver and brain. PINX1/LPTS plays an important mechanical role in maintaining the stability of chromosomal ends by binding to telomerase and its components within the nucleus.

Biological function summary

PINX1/LPTS serves as a regulatory factor that ensures genomic integrity by directly inhibiting telomerase. It is not confirmed part of a specific large protein complex but its interaction with telomerase suggests it functions closely within a group of regulatory proteins. Its activity contributes to cell cycle regulation particularly influencing cellular senescence and apoptosis by controlling telomere length.

Pathways

The role of PINX1/LPTS in telomerase inhibition places it in the context of telomeric maintenance and regulation pathways. It closely associates with proteins such as hTERT the catalytic subunit of telomerase and influences the shelterin complex pathways which protect telomeres from being recognized as DNA damage. This positioning emphasizes its involvement in regulating cell aging and proliferation pathways.

PINX1/LPTS's involvement in telomere length regulation connects it to conditions like cancer where telomerase activity commonly increases. Altered function of PINX1 has been observed in several cancer types influencing tumor growth and progression. Additionally dysregulation of telomere maintenance in which PINX1 plays a part also links it to disorders like dyskeratosis congenita a condition marked by abnormal telomere shortening.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Microtubule-binding protein essential for faithful chromosome segregation. Mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres. Inhibits telomerase activity. May inhibit cell proliferation and act as tumor suppressor.

See full target information PINX1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 15:2518-2534 PubMed40667563

2025

PINX1 inhibits proliferation and cisplatin resistance in nasopharyngeal carcinoma by promoting ILF3 ubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Ni Zhou,Pinggui Gong,Shuilian Wang,Cui He,Liya Hu,Hong Peng

Hereditas 161:27 PubMed39192374

2024

The novel circFKBP8/miR-432-5p/E2F7 cascade functions as a regulatory network in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongkui Jin,Wang Xu,Kunlin Yu,Cailu Luo,Xiaodan Luo,Tao Lian,Changshan Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PINX1/LPTS antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PINX1/LPTS antibody (AB190252)

Immunoprecipitation - Anti-PINX1/LPTS antibody (AB190252)

Western blot - Anti-PINX1/LPTS antibody (AB190252)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise