Rabbit Polyclonal PINX1/LPTS antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PINX1 aa 200-300.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human PINX1 aa 200-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q96BK5
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Orangutan | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes Epitope retrieval with citrate buffer pH 6.0 is recommended for tissue sections. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Orangutan | Dilution info - | Notes - |
Associated Products
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Target data
Function
Microtubule-binding protein essential for faithful chromosome segregation. Mediates TRF1 and TERT accumulation in nucleolus and enhances TRF1 binding to telomeres. Inhibits telomerase activity. May inhibit cell proliferation and act as tumor suppressor.
Alternative names
LPTL, LPTS, PINX1, PIN2/TERF1-interacting telomerase inhibitor 1, Liver-related putative tumor suppressor, Pin2-interacting protein X1, Protein 67-11-3, TRF1-interacting protein 1
Recommended products
Rabbit Polyclonal PINX1/LPTS antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human PINX1 aa 200-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human PINX1 aa 200-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q96BK5
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab190252 was affinity purified using an epitope specific to PINX1/LPTS immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PINX1 also known as LPTS is a nuclear protein known for its role as a telomerase inhibitor. It has a molecular mass of approximately 42 kDa. This protein is expressed in various tissues including the heart liver and brain. PINX1/LPTS plays an important mechanical role in maintaining the stability of chromosomal ends by binding to telomerase and its components within the nucleus.
- Biological function summary
PINX1/LPTS serves as a regulatory factor that ensures genomic integrity by directly inhibiting telomerase. It is not confirmed part of a specific large protein complex but its interaction with telomerase suggests it functions closely within a group of regulatory proteins. Its activity contributes to cell cycle regulation particularly influencing cellular senescence and apoptosis by controlling telomere length.
- Pathways
The role of PINX1/LPTS in telomerase inhibition places it in the context of telomeric maintenance and regulation pathways. It closely associates with proteins such as hTERT the catalytic subunit of telomerase and influences the shelterin complex pathways which protect telomeres from being recognized as DNA damage. This positioning emphasizes its involvement in regulating cell aging and proliferation pathways.
- Associated diseases and disorders
PINX1/LPTS's involvement in telomere length regulation connects it to conditions like cancer where telomerase activity commonly increases. Altered function of PINX1 has been observed in several cancer types influencing tumor growth and progression. Additionally dysregulation of telomere maintenance in which PINX1 plays a part also links it to disorders like dyskeratosis congenita a condition marked by abnormal telomere shortening.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-PINX1/LPTS antibody (ab190252)
All lanes: Western blot - Anti-PINX1/LPTS antibody (ab190252) at 0.4 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: 293T whole cell lysate at 50 µg
Lane 3: Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 3min
Immunoprecipitation - Anti-PINX1/LPTS antibody (ab190252)
Detection of PINX1/LPTS in immunoprecipitates of Jurkat whole cell lysates (1 mg for IP, 20% of IP loaded) using ab190252 at 6 μg/reaction for IP and 1μg/ml for WB detection (Lane 1). Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 minutes.
All lanes: Immunoprecipitation - Anti-PINX1/LPTS antibody (ab190252)
Predicted band size: 37 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PINX1/LPTS antibody (ab190252)
Immunohistochemical (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections using ab190252 at 1/1000 dilution (1 μg/mL).
Downloads
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