Rabbit Polyclonal PIP5K1 alpha/PIP5K1A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PIP5K1A aa 250-400.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PIP5K1A aa 250-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q99755
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
Target data
Function
Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns(4)P/PI4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2/PIP2), a lipid second messenger that regulates several cellular processes such as signal transduction, vesicle trafficking, actin cytoskeleton dynamics, cell adhesion, and cell motility (PubMed:21477596, PubMed:22942276, PubMed:8955136). PtdIns(4,5)P2 can directly act as a second messenger or can be utilized as a precursor to generate other second messengers: inositol 1,4,5-trisphosphate (IP3), diacylglycerol (DAG) or phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3/PIP3) (PubMed:19158393, PubMed:20660631). PIP5K1A-mediated phosphorylation of PtdIns(4)P is the predominant pathway for PtdIns(4,5)P2 synthesis (By similarity). Can also use phosphatidylinositol (PtdIns) as substrate in vitro (PubMed:22942276). Together with PIP5K1C, is required for phagocytosis, both enzymes regulating different types of actin remodeling at sequential steps (By similarity). Promotes particle ingestion by activating the WAS GTPase-binding protein that induces Arp2/3 dependent actin polymerization at the nascent phagocytic cup (By similarity). Together with PIP5K1B, is required, after stimulation by G-protein coupled receptors, for the synthesis of IP3 that will induce stable platelet adhesion (By similarity). Recruited to the plasma membrane by the E-cadherin/beta-catenin complex where it provides the substrate PtdIns(4,5)P2 for the production of PtdIns(3,4,5)P3, IP3 and DAG, that will mobilize internal calcium and drive keratinocyte differentiation (PubMed:19158393). Positively regulates insulin-induced translocation of SLC2A4 to the cell membrane in adipocytes (By similarity). Together with PIP5K1C has a role during embryogenesis (By similarity). Independently of its catalytic activity, is required for membrane ruffling formation, actin organization and focal adhesion formation during directional cell migration by controlling integrin-induced translocation of the small GTPase RAC1 to the plasma membrane (PubMed:20660631). Also functions in the nucleus where it acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs (PubMed:18288197).
Alternative names
Phosphatidylinositol 4-phosphate 5-kinase type-1 alpha, PIP5K1-alpha, PtdIns(4)P-5-kinase 1 alpha, 68 kDa type I phosphatidylinositol 4-phosphate 5-kinase alpha, Phosphatidylinositol 4-phosphate 5-kinase type I alpha, PIP5KIalpha, PIP5K1A
Recommended products
Rabbit Polyclonal PIP5K1 alpha/PIP5K1A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PIP5K1A aa 250-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PIP5K1A aa 250-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q99755
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PIP5K1 alpha also known as Phosphatidylinositol-4-phosphate 5-kinase type 1 alpha (PIP5K1A) is an enzyme that catalyzes the phosphorylation of phosphatidylinositol-4-phosphate (PI4P) to generate phosphatidylinositol-45-bisphosphate (PI(45)P2). The protein has an approximate mass of 68 kDa and is highly expressed in a variety of human tissues including the brain heart and spleen. PIP5K1A plays a significant role in lipid signaling and membrane dynamics impacting a wide array of cellular processes such as cytoskeletal organization and vesicle trafficking.
- Biological function summary
PIP5K1A influences the production of PI(45)P2 an important phosphoinositide involved in numerous cellular functions. PI(45)P2 acts as a substrate for the production of secondary messengers like inositol trisphosphate (IP3) and diacylglycerol (DAG) essential in signal transduction pathways. PIP5K1A is part of a larger protein complex interacting with cytoskeletal elements and regulators of endocytosis and exocytosis. This association highlights its importance in maintaining cellular compartmentalization and function.
- Pathways
PIP5K1A plays a pivotal role in the phosphoinositide signaling pathway and the actin cytoskeleton regulation pathway. It works closely with proteins such as RAC1 a small GTPase involved in actin polymerization to facilitate changes in the cell structure and motility. Additionally PIP5K1A's product PI(45)P2 serves as a precursor for multiple signaling molecules integrating with pathways that mediate cellular responses to various extracellular stimuli.
- Associated diseases and disorders
PIP5K1A's dysregulation links to cancer and neurological disorders. In cancer alterations in PIP5K1A expression or activity can affect cell proliferation and survival interacting with proteins like AKT that drive oncogenic signaling. In the context of neurological disorders its role in synaptic function and plasticity directly connects to conditions like schizophrenia where PI(45)P2 imbalance may contribute to the disorder's pathophysiology. These associations highlight the essential impact of PIP5K1A in maintaining cellular homeostasis and its potential as a therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-PIP5K1 alpha/PIP5K1A antibody (ab122049)
All lanes: Western blot - Anti-PIP5K1 alpha/PIP5K1A antibody (ab122049) at 1/250 dilution
Lane 1: RT-4 cells lysate
Lane 2: U-251 MG cells lysate
Lane 3: Human plasma lysate
Lane 4: Human Liver lysate
Lane 5: Human Tonsil lysate
Predicted band size: 63 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIP5K1 alpha/PIP5K1A antibody (ab122049)
ab122049, at 1/125, staining PIP5K1 alpha/PIP5K1A in paraffin embedded Human tonsil tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-PIP5K1 alpha/PIP5K1A antibody (ab122049)
ab122049, at 4 μg/ml, staining PIP5K1 alpha/PIP5K1A in Human cell line A-431 by Immunofluorescence.
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