AB154818

Anti-PIP5K1 beta antibody [EPR9268]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal PIP5K1 beta antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

STM7, PIP5K1B, Phosphatidylinositol 4-phosphate 5-kinase type-1 beta, PIP5K1-beta, PtdIns(4)P-5-kinase 1 beta, Phosphatidylinositol 4-phosphate 5-kinase type I beta, Protein STM-7, Type I phosphatidylinositol 4-phosphate 5-kinase beta, PIP5KIbeta

1 Images

Unknown

Western blot - Anti-PIP5K1 beta antibody [EPR9268] (AB154818)

All lanes:

Western blot - Anti-PIP5K1 beta antibody [EPR9268] (ab154818) at 1/1000 dilution

Lane 1:

Human placenta tissue lysate at 10 µg

Lane 2:

Human fetal kidney tissue lysate at 10 µg

Predicted band size: 61 kDa

false

Carrier free

Anti-PIP5K1 beta antibody [EPR9268] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9268

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The phosphatidylinositol-4-phosphate 5-kinase type 1 beta (PIP5K1β) also known as PIPK type I beta is an enzyme that plays an important role in the synthesis of phosphatidylinositol 45-bisphosphate (PIP2) from phosphatidylinositol 4-phosphate (PI4P). This enzyme has a molecular weight of approximately 68 kDa. It expresses across various tissues with significant presence in the brain and in hematopoietic cells. The PIP5K1β enzyme is important in regulating the levels of PIP2 a critical lipid in membrane dynamics and signaling.

Biological function summary

PIP5K1β acts within the phosphoinositide signaling pathway which is essential for a range of cellular processes including actin cytoskeleton organization vesicle trafficking and cell survival. It forms part of a complex with other kinases and phosphatases that modulate cellular lipid concentrations. PIP5K1β also contributes to signal transduction processes by participating in the recruitment and activation of proteins that respond to membrane-associated signals.

Pathways

PIP5K1β is heavily involved in the regulation of the phosphoinositide 3-kinase (PI3K) pathway and the actin cytoskeleton pathway. Its product PIP2 serves as a substrate for PI3K which generates phosphatidylinositol-345-trisphosphate (PIP3) an important signaling molecule. PIP5K1β therefore indirectly impacts pathways that involve proteins like AKT and Rac1 which are important to cellular growth and motility.

PIP5K1β connects to cancer and immune system-related disorders. Dysregulation of PIP5K1β activity can lead to aberrant PI3K signaling contributing to oncogenic processes. Additionally it affects proteins such as AKT which plays a role in tumor progression and survival. In immune disorders altered PIP5K1β levels can impact the actin cytoskeleton affecting immune cell dynamics and function.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns(4)P/PI4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2/PIP2), a lipid second messenger that regulates several cellular processes such as signal transduction, vesicle trafficking, actin cytoskeleton dynamics, cell adhesion, and cell motility (By similarity). PtdIns(4,5)P2 can directly act as a second messenger or can be utilized as a precursor to generate other second messengers : inositol 1,4,5-trisphosphate (IP3), diacylglycerol (DAG) or phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3/PIP3) (By similarity). Mediates RAC1-dependent reorganization of actin filaments. Contributes to the activation of phospholipase PLD2. Together with PIP5K1A, is required, after stimulation by G-protein coupled receptors, for the synthesis of IP3 that will induce stable platelet adhesion (By similarity).

See full target information PIP5K1B

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 94: PubMed32376619

2020

Type I Phosphatidylinositol-4-Phosphate 5-Kinases α and γ Play a Key Role in Targeting HIV-1 Pr55 to the Plasma Membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Baptiste Gonzales,Hugues de Rocquigny,Anne Beziau,Stephanie Durand,Julien Burlaud-Gaillard,Antoine Lefebvre,Sandra Krull,Patrick Emond,Denys Brand,Eric Piver

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PIP5K1 beta antibody [EPR9268]

Western blot - Anti-PIP5K1 beta antibody [EPR9268] (AB154818)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise