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AB109192

Anti-PIP5K1C antibody [MAO-R1]

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(11 Publications)

Rabbit Recombinant Monoclonal PIP5K1C antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 11 publications.

View Alternative Names

Kiaa0589, Pip5k1c, Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma, PIP5K1-gamma, PtdIns(4)P-5-kinase 1 gamma, Phosphatidylinositol 4-phosphate 5-kinase type I gamma, PIP5KIgamma

3 Images
Western blot - Anti-PIP5K1C antibody [MAO-R1] (AB109192)
  • WB

Supplier Data

Western blot - Anti-PIP5K1C antibody [MAO-R1] (AB109192)

Lanes 1-4 : Merged signal (red and green). Green - ab109192 observed at 73 kDa. Red - loading control ab8245 observed at 37 kDa.

ab109192 Anti-PIP5K1C antibody [MAO-R1] was shown to specifically react with PIP5K1C in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265545 (knockout cell lysate ab258117) was used. Wild-type and PIP5K1C knockout samples were subjected to SDS-PAGE. ab109192 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PIP5K1C antibody [MAO-R1] (ab109192) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PIP5K1C knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human PIP5K1C knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-pip5k1c-knockout-hela-cell-line-ab265545'>ab265545</a>)

Lane 3:

Neuro-2a cell lysate at 20 µg

Lane 4:

A549 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 73 kDa

Observed band size: 73 kDa

false

Western blot - Anti-PIP5K1C antibody [MAO-R1] (AB109192)
  • WB

Lab

Western blot - Anti-PIP5K1C antibody [MAO-R1] (AB109192)

Lanes 1-4 : Merged signal (red and green). Green - ab109192 observed at 73 kDa. Red - loading control ab8245 observed at 37 kDa.

ab109192 Anti-PIP5K1C antibody [MAO-R1] was shown to specifically react with PIP5K1C in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265545 (knockout cell lysate ab258117) was used. Wild-type and PIP5K1C knockout samples were subjected to SDS-PAGE. ab109192 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PIP5K1C antibody [MAO-R1] (ab109192) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PIP5K1C knockout HeLa cell lysate at 20 µg

Lane 3:

Neuro-2a cell lysate at 20 µg

Lane 4:

A549 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 73 kDa

false

Western blot - Anti-PIP5K1C antibody [MAO-R1] (AB109192)
  • WB

Unknown

Western blot - Anti-PIP5K1C antibody [MAO-R1] (AB109192)

All lanes:

Western blot - Anti-PIP5K1C antibody [MAO-R1] (ab109192) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

Human fetal kidney cell lysate at 10 µg

Lane 3:

C6 cell lysate at 10 µg

Lane 4:

Neuro-2a cell lysate at 10 µg

Lane 5:

293T cell lysate at 10 µg

Predicted band size: 73 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

MAO-R1

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein PIP5K1C also known as phosphatidylinositol-4-phosphate 5-kinase type-1 gamma has a mass of approximately 75 kDa. This enzyme plays a critical role in the phosphatidylinositol signaling pathway where it catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PI4P) to produce phosphatidylinositol 45-bisphosphate (PIP2). PIP5K1C's activity is pivotal for membrane trafficking and it demonstrates widespread expression in tissues including the brain and skeletal muscle.
Biological function summary

PIP5K1C is involved in the regulation of actin cytoskeleton dynamics. It participates as an important enzyme in complexes that modulate actin polymerization and cell adhesion processes. Its function impacts cell motility and maintenance of cell shape contributing significantly to endocytosis and exocytosis. This function highlights the enzyme's role in intracellular signaling and adhesion-related activities.

Pathways

PIP5K1C interacts intricately within the phosphoinositide signaling pathway and impacts the regulation of focal adhesion. It influences other proteins like RAC1 an important regulator of actin polymerization and talin which is involved in linking integrins to the actin cytoskeleton. These interactions place PIP5K1C as an important contributor to the molecular mechanisms that control cell movement and attachment.

Mutations or dysfunctions in PIP5K1C have been associated with neurological disorders such as Charcot-Marie-Tooth disease type 4F and L-fucosidosis. Abnormalities in PIP5K1C expression or activity can disrupt cellular functions and lead to these conditions. In the context of Charcot-Marie-Tooth disease PIP5K1C may interact with dynamin 2 a protein involved in endocytic vesicle fission which further influences the peripheral nervous system pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns(4)P/PI4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2/PIP2), a lipid second messenger that regulates several cellular processes such as signal transduction, vesicle trafficking, actin cytoskeleton dynamics, cell adhesion, and cell motility (PubMed : 14741049, PubMed : 20622009, PubMed : 22942276, PubMed : 9535851). PtdIns(4,5)P2 can directly act as a second messenger or can be utilized as a precursor to generate other second messengers : inositol 1,4,5-trisphosphate (IP3), diacylglycerol (DAG) or phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3/PIP3) (By similarity). PIP5K1A-mediated phosphorylation of PtdIns(4)P is the predominant pathway for PtdIns(4,5)P2 synthesis (By similarity). Together with PIP5K1A, is required for phagocytosis, both enzymes regulating different types of actin remodeling at sequential steps (PubMed : 19153220). Promotes particle attachment by generating the pool of PtdIns(4,5)P2 that induces controlled actin depolymerization to facilitate Fc-gamma-R clustering. Mediates RAC1-dependent reorganization of actin filaments. Required for synaptic vesicle transport (PubMed : 15386003). Controls the plasma membrane pool of PtdIns(4,5)P2 implicated in synaptic vesicle endocytosis and exocytosis (By similarity). Plays a role in endocytosis mediated by clathrin and AP-2 (adaptor protein complex 2) (PubMed : 16707488). Required for clathrin-coated pits assembly at the synapse (By similarity). Participates in cell junction assembly (By similarity). Modulates adherens junctions formation by facilitating CDH1/cadherin trafficking (By similarity). Required for focal adhesion dynamics (PubMed : 12422220). Modulates the targeting of talins (TLN1 and TLN2) to the plasma membrane and their efficient assembly into focal adhesions (By similarity). Regulates the interaction between talins (TLN1 and TLN2) and beta-integrins (By similarity). Required for uropodium formation and retraction of the cell rear during directed migration (PubMed : 17928408). Has a role in growth factor-stimulated directional cell migration and adhesion (PubMed : 17635937). Required for talin assembly into nascent adhesions forming at the leading edge toward the direction of the growth factor (PubMed : 17635937). Negative regulator of T-cell activation and adhesion (PubMed : 20855869). Negatively regulates integrin alpha-L/beta-2 (LFA-1) polarization and adhesion induced by T-cell receptor (PubMed : 20855869). Together with PIP5K1A has a role during embryogenesis and together with PIP5K1B may have a role immediately after birth (PubMed : 17609388, PubMed : 20622009).
See full target information Pip5k1c
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 8: PubMed40441895

2025

Tbx1 plays a critical role in focal adhesion dynamics through paxillin regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Olimpia Iacolare,Rosa Ferrentino,Alessandra Altomonte,Christopher E Turner,Antonio Baldini,Daniela Alfano

Proceedings of the National Academy of Sciences of the United States of America 121:e2401251121 PubMed39136993

2024

IL-13 and IL-17A activate β1 integrin through an NF-kB/Rho kinase/PIP5K1γ pathway to enhance force transmission in airway smooth muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Uyen Ngo,Ying Shi,Prescott Woodruff,Kevan Shokat,William DeGrado,Hyunil Jo,Dean Sheppard,Aparna B Sundaram

Theranostics 12:4386-4398 PubMed35673560

2022

Targeting type Iγ phosphatidylinositol phosphate kinase overcomes oxaliplatin resistance in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Minhao Yu,Hao Wang,Wei Zhao,Xiaoxiao Ge,Wei Huang,Fengjuan Lin,Wenbo Tang,Ang Li,Sailiang Liu,Rong-Kun Li,Shu-Heng Jiang,Junli Xue

Journal of virology 94: PubMed32376619

2020

Type I Phosphatidylinositol-4-Phosphate 5-Kinases α and γ Play a Key Role in Targeting HIV-1 Pr55 to the Plasma Membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Baptiste Gonzales,Hugues de Rocquigny,Anne Beziau,Stephanie Durand,Julien Burlaud-Gaillard,Antoine Lefebvre,Sandra Krull,Patrick Emond,Denys Brand,Eric Piver

The FEBS journal 286:4709-4720 PubMed31276292

2019

SUMOylation is required for PIPK1γ-driven keratinocyte migration and growth.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Ni,Ying Li,Lili Cai,Changsheng Dong,Houshun Fang,Yao Chen,Hui Li,Min Yao,Ning Xiao

Cell reports 27:1991-2001.e5 PubMed31091439

2019

PIP4Ks Suppress Insulin Signaling through a Catalytic-Independent Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Diana G Wang,Marcia N Paddock,Mark R Lundquist,Janet Y Sun,Oksana Mashadova,Solomon Amadiume,Timothy W Bumpus,Cindy Hodakoski,Benjamin D Hopkins,Matthew Fine,Amanda Hill,T Jonathan Yang,Jeremy M Baskin,Lukas E Dow,Lewis C Cantley

EBioMedicine 44:375-386 PubMed31105034

2019

Type Iγ phosphatidylinositol phosphate kinase promotes tumor growth by facilitating Warburg effect in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Peng,Wei Huang,Xiaoxiao Ge,Liqiong Xue,Wei Zhao,Junli Xue

Oncotarget 9:36358-36370 PubMed30555634

2018

The phosphorylation status of PIP5K1C at serine 448 can be predictive for invasive ductal carcinoma of the breast.

Applications

Unspecified application

Species

Unspecified reactive species

Nisha Durand,Sahra Borges,Tavia Hall,Ligia Bastea,Heike Döppler,Brandy H Edenfield,E Aubrey Thompson,Xochiquetzal Geiger,Peter Storz

The Journal of biological chemistry 291:25729-25741 PubMed27780861

2016

p70S6K1 (S6K1)-mediated Phosphorylation Regulates Phosphatidylinositol 4-Phosphate 5-Kinase Type I γ Degradation and Cell Invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Naser Jafari,Qiaodan Zheng,Liqing Li,Wei Li,Lei Qi,Jianyong Xiao,Tianyan Gao,Cai Huang

The Journal of neuroscience : the official journal of the Society for Neuroscience 34:7165-78 PubMed24849351

2014

Steering neuronal growth cones by shifting the imbalance between exocytosis and endocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Takuro Tojima,Rurika Itofusa,Hiroyuki Kamiguchi
View all publications
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