Rabbit Recombinant Monoclonal LIRB3 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse samples.
Images
![Western blot - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (AB283700), expandable thumbnail](https://content.abcam.com/products/images/pirb-antibody-epr24885-31-bsa-and-azide-free-ab283700--western-blot-img168183.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (AB283700)")
![Western blot - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (AB283700), expandable thumbnail](https://content.abcam.com/products/images/pirb-antibody-epr24885-31-bsa-and-azide-free-ab283700--western-blot-img169837.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (AB283700)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (AB283700), expandable thumbnail](https://content.abcam.com/products/images/pirb-antibody-epr24885-31-bsa-and-azide-free-ab283700--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img172747.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (AB283700)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (AB283700), expandable thumbnail](https://content.abcam.com/products/images/pirb-antibody-epr24885-31-bsa-and-azide-free-ab283700--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img167009.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (AB283700)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (AB283700), expandable thumbnail](https://content.abcam.com/products/images/pirb-antibody-epr24885-31-bsa-and-azide-free-ab283700--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img164937.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (AB283700)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt | IP | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Target data
Function
May act as receptor for class I MHC antigens. Becomes activated upon coligation of LILRB3 and immune receptors, such as FCGR2B and the B-cell receptor. Down-regulates antigen-induced B-cell activation by recruiting phosphatases to its immunoreceptor tyrosine-based inhibitor motifs (ITIM).
Alternative names
Pirb, Lilrb3, Leukocyte immunoglobulin-like receptor subfamily B member 3, LIR-3, Leukocyte immunoglobulin-like receptor 3, Cell-surface glycoprotein p91, Paired immunoglobulin-like receptor B, PIR-B
Recommended products
Rabbit Recombinant Monoclonal LIRB3 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR24885-31
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab283700 the carrier-free version of Anti-PIRB antibody [EPR24885-31] ab271086.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Paired immunoglobulin-like receptor B commonly known as PIR-B is an inhibitory receptor expressed in various immune cells like macrophages dendritic cells and B cells. PIR-B often weighs around 129 kDa. It consists of extracellular immunoglobulin domains and intracellular immunoreceptor tyrosine-based inhibition motifs (ITIMs). This receptor is a part of the immune system machinery where it interacts with major histocompatibility complex (MHC) class I molecules which makes it a point of interest in understanding immune responses and signaling.
- Biological function summary
PIR-B plays a role in immune regulation and tolerance. It is involved in inhibiting signaling pathways that activate immune cells. PIR-B forms a part of signal regulatory complex working alongside other receptors in the immune system to maintain a balanced immune response. By interacting with ITIMs PIR-B recruits phosphatases which de-phosphorylate signaling molecules leading to reduced cellular activation. This inhibitory mechanism ensures the prevention of excessive immune responses which can cause tissue damage.
- Pathways
The immune regulation by PIR-B is important in innate and adaptive immunity. PIR-B integrates its function into pathways such as the negative regulation of immune effector processes and signal transduction. PIR-B has similarity with proteins like CD300 and ILT family involved in controlling immune response by hindering excessive cellular activation. Its action contributes significantly to homeostasis by regulating signal transduction processes during immune responses.
- Associated diseases and disorders
PIR-B has a relationship with autoimmune diseases and certain cancers. Its expression and inhibitory function relate to conditions like rheumatoid arthritis and lymphoma. In rheumatoid arthritis PIR-B's interaction with MHC class I molecules and phosphatases like SHP-1 and SHP-2 modulates the immune response and may contribute to disease pathogenesis. In some cancers the dampening of immune responses by PIR-B might facilitate tumor evasion from immune surveillance making it a potential target for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (ab283700), expandable thumbnail](https://content.abcam.com/products/images/pirb-antibody-epr24885-31-bsa-and-azide-free-ab283700--western-blot-img168183.jpg "Western blot - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (ab283700)")
Western blot - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (ab283700)
This data was developed using Anti-PIRB antibody [EPR24885-31] ab271086, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: The antibody detects isoform of PirB around 40kDa which consistent with what has been described in the literature (PMID: 31388006)
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 136 seconds
All lanes: Western blot - Anti-PIRB antibody [EPR24885-31] (Anti-PIRB antibody [EPR24885-31] ab271086) at 1/1000 dilution
Lane 1: WEHI-3 (mouse leukemia lymphoblast), whole cell lysate at 20 µg
Lane 2: WEHI-3 whole cell lysate treated with Protein Deglycosylation MIX at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
![Western blot - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (ab283700), expandable thumbnail](https://content.abcam.com/products/images/pirb-antibody-epr24885-31-bsa-and-azide-free-ab283700--western-blot-img169837.jpg "Western blot - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (ab283700)")
Western blot - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (ab283700)
This data was developed using Anti-PIRB antibody [EPR24885-31] ab271086, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: Negative control: EL4 (PMID: 21555536)
The antibody detects isoform of PirB around 40kDa which consistent with what has been described in the literature (PMID: 31388006)
The blot of lane 2&3 was developed using a higher sensitivity ECL substrate.
Exposure time: Lane 1: 92 seconds Lane 2&3: 169 seconds
All lanes: Western blot - Anti-PIRB antibody [EPR24885-31] (Anti-PIRB antibody [EPR24885-31] ab271086) at 1/1000 dilution
Lane 1: Mouse spleen tissue lysate at 20 µg
Lane 2: WEHI-3 (mouse leukemia lymphoblast), whole cell lysate at 20 µg
Lane 3: EL4 (mouse lymphoma T lymphocyte), whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (ab283700), expandable thumbnail](https://content.abcam.com/products/images/pirb-antibody-epr24885-31-bsa-and-azide-free-ab283700--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img172747.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (ab283700)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (ab283700)
This data was developed using Anti-PIRB antibody [EPR24885-31] ab271086, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling Pirb with Anti-PIRB antibody [EPR24885-31] ab271086 at 1/100 (5.47 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: No staining on mouse cerebrum. The section was incubated with Anti-PIRB antibody [EPR24885-31] ab271086 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (ab283700), expandable thumbnail](https://content.abcam.com/products/images/pirb-antibody-epr24885-31-bsa-and-azide-free-ab283700--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img167009.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (ab283700)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (ab283700)
This data was developed using Anti-PIRB antibody [EPR24885-31] ab271086, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse pancreatic carcinoma and paired adjacent tissue tissue labelling Pirb with Anti-PIRB antibody [EPR24885-31] ab271086 at 1/100 (5.47 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on immune cells of mouse pancreatic carcinoma and paired adjacent tissue. The section was incubated with Anti-PIRB antibody [EPR24885-31] ab271086 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (ab283700), expandable thumbnail](https://content.abcam.com/products/images/pirb-antibody-epr24885-31-bsa-and-azide-free-ab283700--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img164937.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (ab283700)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (ab283700)
This data was developed using Anti-PIRB antibody [EPR24885-31] ab271086, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labelling Pirb with Anti-PIRB antibody [EPR24885-31] ab271086 at 1/100 (5.47 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on immune cells of mouse stomach. The section was incubated with Anti-PIRB antibody [EPR24885-31] ab271086 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (ab283700), expandable thumbnail](https://content.abcam.com/products/images/pirb-antibody-epr24885-31-bsa-and-azide-free-ab283700--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img180261.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (ab283700)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (ab283700)
This data was developed using Anti-PIRB antibody [EPR24885-31] ab271086, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling Pirb with Anti-PIRB antibody [EPR24885-31] ab271086 at 1/100 (5.47 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse spleen. The section was incubated with Anti-PIRB antibody [EPR24885-31] ab271086 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (ab283700), expandable thumbnail](https://content.abcam.com/products/images/pirb-antibody-epr24885-31-bsa-and-azide-free-ab283700--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img180261.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (ab283700)")
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