Anti-PIRB antibody [EPR24885-31] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (AB283700)

This data was developed using ab271086, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling Pirb with ab271086 at 1/100 (5.47 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : No staining on mouse cerebrum. The section was incubated with ab271086 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (AB283700)

This data was developed using ab271086, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse pancreatic carcinoma and paired adjacent tissue tissue labelling Pirb with ab271086 at 1/100 (5.47 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on immune cells of mouse pancreatic carcinoma and paired adjacent tissue. The section was incubated with ab271086 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (AB283700)

This data was developed using ab271086, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labelling Pirb with ab271086 at 1/100 (5.47 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on immune cells of mouse stomach. The section was incubated with ab271086 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (AB283700)

This data was developed using ab271086, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling Pirb with ab271086 at 1/100 (5.47 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse spleen. The section was incubated with ab271086 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• WB

Lab

Western blot - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (AB283700)

This data was developed using ab271086, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : The antibody detects isoform of PirB around 40kDa which consistent with what has been described in the literature (PMID : 31388006)

This blot was developed using a higher sensitivity ECL substrate.

Exposure time : 136 seconds

All lanes:

Western blot - Anti-PIRB antibody [EPR24885-31] (<a href='/en-us/products/primary-antibodies/pirb-antibody-epr24885-31-ab271086'>ab271086</a>) at 1/1000 dilution

Lane 1:

WEHI-3 (mouse leukemia lymphoblast), whole cell lysate at 20 µg

Lane 2:

WEHI-3 whole cell lysate treated with Protein Deglycosylation MIX at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

• WB

Lab

Western blot - Anti-PIRB antibody [EPR24885-31] - BSA and Azide free (AB283700)

This data was developed using ab271086, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : Negative control : EL4 (PMID : 21555536)

The antibody detects isoform of PirB around 40kDa which consistent with what has been described in the literature (PMID : 31388006)

The blot of lane 2&3 was developed using a higher sensitivity ECL substrate.

Exposure time : Lane 1 : 92 seconds Lane 2&3 : 169 seconds

All lanes:

Western blot - Anti-PIRB antibody [EPR24885-31] (<a href='/en-us/products/primary-antibodies/pirb-antibody-epr24885-31-ab271086'>ab271086</a>) at 1/1000 dilution

Lane 1:

Mouse spleen tissue lysate at 20 µg

Lane 2:

WEHI-3 (mouse leukemia lymphoblast), whole cell lysate at 20 µg

Lane 3:

EL4 (mouse lymphoma T lymphocyte), whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false