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AB189247

Anti-Pirh2 antibody [EPR14980]

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(4 Publications)

Rabbit Recombinant Monoclonal Pirh2 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 4 publications.

View Alternative Names

ARNIP, CHIMP, PIRH2, RNF199, ZNF363, RCHY1, RING finger and CHY zinc finger domain-containing protein 1, Androgen receptor N-terminal-interacting protein, CH-rich-interacting match with PLAG1, E3 ubiquitin-protein ligase Pirh2, RING finger protein 199, RING-type E3 ubiquitin transferase RCHY1, Zinc finger protein 363, p53-induced RING-H2 protein, hPirh2

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-Pirh2 antibody [EPR14980] (AB189247)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Pirh2 antibody [EPR14980] (AB189247)

Immunofluorescence analysis of 4% paraformaldehyde-fixed HeLa cells labeling Pirh2 with ab189247 at 1/100 dilution. Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody (red). Slide is counter stained with DAPI (blue).

Western blot - Anti-Pirh2 antibody [EPR14980] (AB189247)
  • WB

Lab

Western blot - Anti-Pirh2 antibody [EPR14980] (AB189247)

Lanes 1-4 : Merged signal (red and green). Green - ab189247 observed at 30 kDa. Red - loading control ab8245 observed at 37 kDa.

ab189247 Recombinant Anti-Pirh2 antibody [EPR14980] was shown to specifically react with RCHY1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265478 (knockout cell lysate ab258171) was used. Wild-type and RCHY1 knockout samples were subjected to SDS-PAGE. ab189247 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Pirh2 antibody [EPR14980] (ab189247) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

RCHY1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human RCHY1 (Pirh2) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-rchy1-pirh2-knockout-hela-cell-line-ab265478'>ab265478</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 30 kDa

Observed band size: 30 kDa

false

Western blot - Anti-Pirh2 antibody [EPR14980] (AB189247)
  • WB

Supplier Data

Western blot - Anti-Pirh2 antibody [EPR14980] (AB189247)

All lanes:

Western blot - Anti-Pirh2 antibody [EPR14980] (ab189247) at 1/1000 dilution

Lane 1:

293T cell lysate at 20 µg

Lane 2:

LnCaP cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 30 kDa

false

Western blot - Anti-Pirh2 antibody [EPR14980] (AB189247)
  • WB

Supplier Data

Western blot - Anti-Pirh2 antibody [EPR14980] (AB189247)

All lanes:

Western blot - Anti-Pirh2 antibody [EPR14980] (ab189247) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

Raw 264.7 cell lysate at 10 µg

Lane 3:

NIH/3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 30 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pirh2 antibody [EPR14980] (AB189247)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pirh2 antibody [EPR14980] (AB189247)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Pirh2 with ab189247 at 1/50 dilution. The slide is counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14980

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pirh2 also known as RCHY1 is a protein with E3 ubiquitin ligase activity. It has a molecular mass of approximately 34 kDa. Pirh2 targets proteins for degradation by the ubiquitin-proteasome system regulating the levels of proteins like p53. Expression of Pirh2 occurs in various tissues including liver and heart. It helps maintain cellular protein homeostasis by marking specific substrates for degradation.
Biological function summary

Pirh2 influences the regulation of protein stability by tagging proteins for degradation. Besides p53 it acts on other substrates and engages in various cellular processes. Pirh2 operates independently and its presence affects cellular responses to stress and DNA damage. It modulates cell cycle progression and apoptosis impacting cell fate decisions.

Pathways

Pirh2 plays an important role in the p53 signaling pathway an essential cell regulatory network. By mediating the ubiquitination of p53 Pirh2 affects cell survival and division under stress conditions. Interaction with proteins such as Mdm2 further regulates p53 activity impacting the cell's ability to respond to DNA damage and carcinogen exposure.

Research connects Pirh2 with the development of cancers. Its interaction with the tumor suppressor protein p53 poses effects on tumorigenesis by altering p53 stability and function. Overexpression of Pirh2 has connections to several cancer types including breast and liver cancers. These associations suggest Pirh2 as a potential target for therapeutic interventions that aim at modulating the p53 pathway to treat cancer.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that mediates ubiquitination of target proteins, including p53/TP53, TP73, HDAC1 and CDKN1B (PubMed : 16914734, PubMed : 17721809, PubMed : 18006823, PubMed : 19043414, PubMed : 19483087, PubMed : 21994467). Mediates ubiquitination and degradation of p53/TP53; preferentially acts on tetrameric p53/TP53 (PubMed : 19043414, PubMed : 19483087). Catalyzes monoubiquitinates the translesion DNA polymerase POLH (PubMed : 21791603). Involved in the ribosome-associated quality control (RQC) pathway, which mediates the extraction of incompletely synthesized nascent chains from stalled ribosomes : RCHY1 acts downstream of NEMF and recognizes CAT tails associated with stalled nascent chains, leading to their ubiquitination and degradation (PubMed : 33909987).. Isoform 4. Has no E3 ubiquitin-protein ligase activity.
See full target information RCHY1
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 9:e22084 PubMed38058614

2023

KIAA1429 facilitates progression of hepatocellular carcinoma by modulating m6A levels in HPN.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Meng,Wenwen Yang,Jinchao Li,Wei Chai

Cell reports 42:113100 PubMed37676773

2023

Mechanism and evolutionary origins of alanine-tail C-degron recognition by E3 ligases Pirh2 and CRL2-KLHDC10.

Applications

Unspecified application

Species

Unspecified reactive species

Pratik Rajendra Patil,A Maxwell Burroughs,Mohit Misra,Federico Cerullo,Carlos Costas-Insua,Hao-Chih Hung,Ivan Dikic,L Aravind,Claudio A P Joazeiro

Molecular cell 81:2112-2122.e7 PubMed33909987

2021

Convergence of mammalian RQC and C-end rule proteolytic pathways via alanine tailing.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Thrun,Aitor Garzia,Yu Kigoshi-Tansho,Pratik R Patil,Charles S Umbaugh,Teresa Dallinger,Jia Liu,Sylvia Kreger,Annarita Patrizi,Gregory A Cox,Thomas Tuschl,Claudio A P Joazeiro

Protein & cell 9:770-784 PubMed29441489

2018

Pirh2 mediates the sensitivity of myeloma cells to bortezomib via canonical NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Li Yang,Jing Chen,Xiaoyan Han,Enfan Zhang,Xi Huang,Xing Guo,Qingxiao Chen,Wenjun Wu,Gaofeng Zheng,Donghua He,Yi Zhao,Yang Yang,Jingsong He,Zhen Cai
View all publications
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