AB227280

Anti-Pirin/PIR antibody

Name: Anti-Pirin/PIR antibody (ab227280) | Abcam
Brand: Abcam Limited
SKU: AB227280
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Pirin/PIR antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PIR.

View Alternative Names

Pirin, Probable quercetinase, PIR

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pirin/PIR antibody (AB227280)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pirin/PIR antibody (AB227280)

Paraffin-embedded MPG xenograft tissue stained for Pirin/PIR using ab227280 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Pirin/PIR antibody (AB227280)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Pirin/PIR antibody (AB227280)

Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Pirin/PIR (green) using ab227280 at 1/500 dilution in ICC/IF. Nuclear Counterstain : Hoechst 33342 (blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pirin/PIR antibody (AB227280)

Paraffin-embedded mouse liver tissue stained for Pirin/PIR using ab227280 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pirin/PIR antibody (AB227280)

Paraffin-embedded rat brain stem tissue stained for Pirin/PIR using ab227280 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Pirin/PIR antibody (AB227280)

12% SDS-PAGE gel.

All lanes:

Western blot - Anti-Pirin/PIR antibody (ab227280) at 1000 Cells

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Predicted band size: 32 kDa

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Supplier Data

Western blot - Anti-Pirin/PIR antibody (AB227280)

12% SDS-PAGE gel.

All lanes:

Western blot - Anti-Pirin/PIR antibody (ab227280) at 1/1000 dilution

All lanes:

Mouse liver tissue lysate at 50 µg

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human PIR. The exact immunogen used to generate this antibody is proprietary information.

O00625

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pirin also known as PIR protein is a nuclear iron-dependent non-heme oxidoreductase involved in various cellular processes. The PIR protein sometimes called PIR-B or PIR-A/B has a molecular mass of approximately 32 kDa. It expresses in many human tissues including the brain heart and lungs. The PIR protein plays significant roles in stress response and regulation of transcription factors making it a subject of interest in cellular regulation and signal transduction research.

Biological function summary

PIR protein associates with transcriptional co-factors and mediates gene expression regulation impacting cellular metabolism and growth. It interacts with several alternative forms such as PIR alternative and antipirin contributing to its diverse functionality. PIR often forms part of transcription complexes facilitating the modulation of gene networks essential for maintaining cellular homeostasis.

Pathways

PIR protein participates in significant biological processes like the NF-kB signaling and apoptosis regulatory pathways. In the NF-kB pathway PIR contributes to transcription factor activation while in apoptosis it influences pathways promoting cell survival or programmed cell death. PIR protein relates closely to proteins such as Bcl-3 and related transcription factors shaping cellular responses to external stimuli and maintaining balance between cell survival and death.

PIR protein associates with various cancers and neurodegenerative diseases. In cancer PIR's role in transcription regulation impacts tumor progression and metastasis interacting with proteins like c-Myc. In neurodegeneration abnormalities in PIR expression or function link to disorders such as Alzheimer's disease where it interacts with tau protein exacerbating the disease pathology. Understanding PIR's relationship to these conditions might provide insights into new therapeutic approaches.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional coregulator of NF-kappa-B which facilitates binding of NF-kappa-B proteins to target kappa-B genes in a redox-state-dependent manner. May be required for efficient terminal myeloid maturation of hematopoietic cells. Has quercetin 2,3-dioxygenase activity (in vitro).

See full target information PIR

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Discover. Oncology 12:20 PubMed35201449

2021

Diaphanous related formin 3 knockdown suppresses cell proliferation and metastasis of osteosarcoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zehua Zhang,Fei Dai,Fei Luo,Wenjie Wu,Shuai Zhang,Rui Zhou,Jianzhong Xu,Qiang Zhou,Lei Song

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Pirin/PIR antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pirin/PIR antibody (AB227280)

Immunocytochemistry/ Immunofluorescence - Anti-Pirin/PIR antibody (AB227280)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pirin/PIR antibody (AB227280)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pirin/PIR antibody (AB227280)

Western blot - Anti-Pirin/PIR antibody (AB227280)

Western blot - Anti-Pirin/PIR antibody (AB227280)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise