AB37036

Anti-PIST antibody

Name: Anti-PIST antibody (ab37036) | Abcam
Brand: Abcam Limited
SKU: AB37036
Rating: 5 (1 reviews)

(1 Review)

(9 Publications)

Rabbit Polyclonal PIST antibody. Suitable for ICC, WB, IHC-P and reacts with Rat samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human GOPC.

View Alternative Names

CAL, FIG, GOPC, Golgi-associated PDZ and coiled-coil motif-containing protein, CFTR-associated ligand, Fused in glioblastoma, PDZ protein interacting specifically with TC10, PIST

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody (AB37036)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody (AB37036)

ab37036 at 1 μg/ml staining PIST in rat colon tissue by IHC.

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Immunocytochemistry - Anti-PIST antibody (AB37036)

Immunofluorescence of PIST in Rat Colon cells using ab37036 at 10 ug/ml.

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Western blot - Anti-PIST antibody (AB37036)

Lane 1:

Western blot - Anti-PIST antibody (ab37036) at 1 µg/mL

Lane 2:

Western blot - Anti-PIST antibody (ab37036) at 2 µg/mL

Lane 3:

Western blot - Anti-PIST antibody (ab37036) at 4 µg/mL

All lanes:

rat colon cell lysate at 15 µg

Predicted band size: 51 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat

Applications

WB, IHC-P, ICC

applications

Immunogen

Synthetic Peptide within Human GOPC. The exact immunogen used to generate this antibody is proprietary information.

Q9HD26

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "10 µg/mL", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-4 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PIST also known as PDZ domain-containing protein 8 (GOPC) is a protein with a molecular weight of approximately 55 kDa. This protein is found in multiple tissues with higher expression in the brain and testes. PIST interacts with Golgi membranes and serves as a scaffold in cellular processes by linking transmembrane proteins to different signaling pathways. Its main functional domain the PDZ domain facilitates specific binding to target proteins allowing it to play a versatile role in maintaining cellular functions.

Biological function summary

PIST participates in protein trafficking and sorting. It associates with the complex that regulates vesicular transport ensuring protein delivery to proper cellular locations. In neuronal cells PIST influences the trafficking of receptors like AMPA and NMDA which are important for synaptic function. The involvement of PIST in protein trafficking highlights its significance in cellular organization and communication.

Pathways

PIST engages in interactions within the Wnt and PI3K/Akt signaling pathways. In the Wnt pathway PIST supports the stabilization and trafficking of receptors critical for signal transduction. In the PI3K/Akt pathway it influences cell survival and proliferation by modulating receptor localization and function. Proteins such as Dishevelled and PTEN are often associated with PIST's activity in these pathways highlighting its diverse biological importance.

PIST shows connections to conditions like cancer and neurodegenerative disorders. In cancer deregulation of PIST has been observed potentially impacting cell proliferation and survival since it interacts with proteins like EGFR and other growth factor receptors. In neurodegenerative diseases abnormal PIST function may disrupt synaptic signaling linking it to disorders like Alzheimer's through its interactions with synaptic proteins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in intracellular protein trafficking and degradation (PubMed : 11707463, PubMed : 14570915, PubMed : 15358775). May regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating cell surface expression of both channels (By similarity). May also regulate the intracellular trafficking of the ADR1B receptor (PubMed : 15358775). May play a role in autophagy (By similarity). Together with MARCHF2 mediates the ubiquitination and lysosomal degradation of CFTR (PubMed : 23818989). Overexpression results in CFTR intracellular retention and lysosomaldegradation in the lysosomes (PubMed : 11707463, PubMed : 14570915).

See full target information GOPC

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed36942942

2023

The Slingshot phosphatase 2 is required for acrosome biogenesis during spermatogenesis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Xu,Xianwei Su,Kailun Fang,Yue Lv,Tao Huang,Mengjing Li,Ziqi Wang,Yingying Yin,Tahir Muhammad,Shangming Liu,Xiangfeng Chen,Jing Jiang,Jinsong Li,Wai-Yee Chan,Jinlong Ma,Gang Lu,Zi-Jiang Chen,Hongbin Liu

Infection and immunity 91:e0050522 PubMed36511702

2022

Distinct Molecular Features of NleG Type 3 Secreted Effectors Allow for Different Roles during Citrobacter rodentium Infection in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Georgy Popov,Aline Fiebig-Comyn,Lukas Syriste,Dustin J Little,Tatiana Skarina,Peter J Stogios,Sarah Birstonas,Brian K Coombes,Alexei Savchenko

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 16:761-783 PubMed31073978

2019

PDZ Scaffold Protein CAL Couples with Metabotropic Glutamate Receptor 5 to Protect Against Cell Apoptosis and Is a Potential Target in the Treatment of Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Yuan Luo,Su Qian Xing,Ping Zhu,Chen Guang Zhang,Hui Min Yang,Nicholas Van Halm-Lutterodt,Li Gu,Hong Zhang

Oncology letters 14:4483-4490 PubMed29085445

2017

Low expression of the GOPC is a poor prognostic marker in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Nobuyoshi Ohara,Naotsugu Haraguchi,Jun Koseki,Yujiro Nishizawa,Kenji Kawai,Hidekazu Takahashi,Junichi Nishimura,Taishi Hata,Tsunekazu Mizushima,Hirofumi Yamamoto,Hideshi Ishii,Yuichiro Doki,Masaki Mori

Biology of reproduction 96:587-597 PubMed28339613

2017

A nonsense mutation in Ccdc62 gene is responsible for spermiogenesis defects and male infertility in repro29/repro29 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yuchi Li,Cailing Li,Shouren Lin,Bo Yang,Weiren Huang,Hanwei Wu,Yuanbin Chen,Lihua Yang,Manling Luo,Huan Guo,Jianbo Chen,Tiantian Wang,Qian Ma,Yanli Gu,Lisha Mou,Zhimao Jiang,Jun Xia,Yaoting Gui

Cell death & disease 8:e2532 PubMed28055014

2017

Globozoospermia and lack of acrosome formation in GM130-deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Han,Chunyi Liu,Lianjun Zhang,Min Chen,Yang Zhou,Yan Qin,Yaqing Wang,Min Chen,Shuguang Duo,Xiuhong Cui,Shilai Bao,Fei Gao

Development (Cambridge, England) 144:441-451 PubMed28003215

2016

Sirt1 regulates acrosome biogenesis by modulating autophagic flux during spermiogenesis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Liu,Zhenhua Song,Lina Wang,Haiyan Yu,Weixiao Liu,Yongliang Shang,Zhiliang Xu,Haichao Zhao,Fengyi Gao,Jiamin Wen,Linan Zhao,Yaoting Gui,Jianwei Jiao,Fei Gao,Wei Li

Cell research 24:852-69 PubMed24853953

2014

Atg7 is required for acrosome biogenesis during spermatogenesis in mice.

Applications

Unspecified application

Species

Mouse

Hongna Wang,Haifeng Wan,Xixia Li,Weixiao Liu,Qi Chen,Yaqing Wang,Lin Yang,Hongmei Tang,Xiujun Zhang,Enkui Duan,Xiaoyang Zhao,Fei Gao,Wei Li

The Journal of clinical investigation 119:802-12 PubMed19258705

2009

PICK1 deficiency causes male infertility in mice by disrupting acrosome formation.

Applications

ICC/IF, ICC/IF

Species

Rat, Mouse

Nan Xiao,Chuen Kam,Chong Shen,Wenying Jin,Junqi Wang,Kwong Man Lee,Liwen Jiang,Jun Xia

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PIST antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody (AB37036)

Immunocytochemistry - Anti-PIST antibody (AB37036)

Western blot - Anti-PIST antibody (AB37036)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise