JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB109119

Anti-PIST antibody [EPR4079]

Be the first to review this product! Submit a review

|

(6 Publications)

Rabbit Recombinant Monoclonal PIST antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 6 publications.

View Alternative Names

CAL, FIG, GOPC, Golgi-associated PDZ and coiled-coil motif-containing protein, CFTR-associated ligand, Fused in glioblastoma, PDZ protein interacting specifically with TC10, PIST

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4079] (AB109119)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4079] (AB109119)

Immunohistochemical staining of PIST expression in paraffin-embedded Human stomach tissue using ab109119 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4079] (AB109119)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4079] (AB109119)

ab109119 showing positive staining in Normal brain tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4079] (AB109119)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4079] (AB109119)

ab109119 showing positive staining in Normal kidney tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4079] (AB109119)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4079] (AB109119)

ab109119 showing positive staining in Normal liver tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4079] (AB109119)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4079] (AB109119)

ab109119 showing positive staining in Normal ovary tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4079] (AB109119)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4079] (AB109119)

ab109119 showing positive staining in Normal breast tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-PIST antibody [EPR4079] (AB109119)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PIST antibody [EPR4079] (AB109119)

ab109119 stained HeLa cells. The cells were 4% formaldhyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109119 at 1/100) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43μM for 1hour at room temperature.

Western blot - Anti-PIST antibody [EPR4079] (AB109119)
  • WB

Unknown

Western blot - Anti-PIST antibody [EPR4079] (AB109119)

All lanes:

Western blot - Anti-PIST antibody [EPR4079] (ab109119) at 1/1000 dilution

Lane 1:

A375 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Fetal kidney lysate at 10 µg

Lane 4:

PC-3 cell lysate at 10 µg

Lane 5:

Human prostate lysate at 10 µg

Predicted band size: 51 kDa

false

  • Carrier free

    Anti-PIST antibody [EPR4079] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4079

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB37036

Anti-PIST antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody (AB37036)

  • 5
  • 1
Primary Antibodies

AB133472

Anti-PIST antibody [EPR4080(2)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4080(2)] (AB133472)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB37266

Anti-Giantin antibody [9B6] - Golgi Marker

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [9B6] - Golgi Marker (AB37266)

  • 4
  • 15
Primary Antibodies

AB52649

Anti-GM130 antibody [EP892Y] - cis-Golgi Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunoprecipitation - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (AB52649)

  • 5
  • 39
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB115777

Anti-beta Actin antibody [SP124] - Cytoskeleton Marker

20ul selling size|RabMAb|Lab Essentials|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (AB115777)

  • 4
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PIST also known as PDZ domain-containing protein 8 (GOPC) is a protein with a molecular weight of approximately 55 kDa. This protein is found in multiple tissues with higher expression in the brain and testes. PIST interacts with Golgi membranes and serves as a scaffold in cellular processes by linking transmembrane proteins to different signaling pathways. Its main functional domain the PDZ domain facilitates specific binding to target proteins allowing it to play a versatile role in maintaining cellular functions.
Biological function summary

PIST participates in protein trafficking and sorting. It associates with the complex that regulates vesicular transport ensuring protein delivery to proper cellular locations. In neuronal cells PIST influences the trafficking of receptors like AMPA and NMDA which are important for synaptic function. The involvement of PIST in protein trafficking highlights its significance in cellular organization and communication.

Pathways

PIST engages in interactions within the Wnt and PI3K/Akt signaling pathways. In the Wnt pathway PIST supports the stabilization and trafficking of receptors critical for signal transduction. In the PI3K/Akt pathway it influences cell survival and proliferation by modulating receptor localization and function. Proteins such as Dishevelled and PTEN are often associated with PIST's activity in these pathways highlighting its diverse biological importance.

PIST shows connections to conditions like cancer and neurodegenerative disorders. In cancer deregulation of PIST has been observed potentially impacting cell proliferation and survival since it interacts with proteins like EGFR and other growth factor receptors. In neurodegenerative diseases abnormal PIST function may disrupt synaptic signaling linking it to disorders like Alzheimer's through its interactions with synaptic proteins.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Plays a role in intracellular protein trafficking and degradation (PubMed : 11707463, PubMed : 14570915, PubMed : 15358775). May regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating cell surface expression of both channels (By similarity). May also regulate the intracellular trafficking of the ADR1B receptor (PubMed : 15358775). May play a role in autophagy (By similarity). Together with MARCHF2 mediates the ubiquitination and lysosomal degradation of CFTR (PubMed : 23818989). Overexpression results in CFTR intracellular retention and lysosomaldegradation in the lysosomes (PubMed : 11707463, PubMed : 14570915).
See full target information GOPC
style
left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cellular and molecular life sciences : CMLS 82:4 PubMed39680136

2024

Golgi pH elevation due to loss of V-ATPase subunit V0a2 function correlates with tissue-specific glycosylation changes and globozoospermia.

Applications

Unspecified application

Species

Unspecified reactive species

Johannes Kopp,Denise Jahn,Guido Vogt,Anthi Psoma,Edoardo Ratto,Willy Morelle,Nina Stelzer,Ingrid Hausser,Anne Hoffmann,Miguel Rodriguez de Los Santos,Leonard A Koch,Björn Fischer-Zirnsak,Christian Thiel,Wilhelm Palm,David Meierhofer,Geert van den Bogaart,François Foulquier,Andreas Meinhardt,Uwe Kornak

Acta biochimica et biophysica Sinica 55:1618-1629 PubMed37715489

2023

CTNNAL1 deficiency suppresses CFTR expression in HDM-induced asthma mouse model through ROCK1-CAL signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Di Wu,Jiahui Zhu,Fang Yang,Riwang Li,Lexin Liu,Dahai Liu,Chi Liu,Xiangping Qu,Huijun Liu,Ming Ji,Xiaoqun Qin,Lan Hua,Yang Xiang

Journal of radiation research 64:33-43 PubMed36300343

2022

AKBA inhibits radiotherapy resistance in lung cancer by inhibiting maspin methylation and regulating the AKT/FOXO1/p21 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chun Gong,Wei Li,Jing Wu,Yao-Yao Li,Yi Ma,Li-Wen Tang

The Journal of cell biology 219: PubMed31825461

2019

Regulated resurfacing of a somatostatin receptor storage compartment fine-tunes pituitary secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Walaa Alshafie,Vincent Francis,Klaudia Bednarz,Yingzhou Edward Pan,Thomas Stroh,Peter S McPherson

The Journal of biological chemistry 290:4447-63 PubMed25561724

2015

Acetylation of TUG protein promotes the accumulation of GLUT4 glucose transporters in an insulin-responsive intracellular compartment.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan P Belman,Rachel R Bian,Estifanos N Habtemichael,Don T Li,Michael J Jurczak,Abel Alcázar-Román,Leah J McNally,Gerald I Shulman,Jonathan S Bogan

The Journal of biological chemistry 288:20135-50 PubMed23744065

2013

Enhanced fasting glucose turnover in mice with disrupted action of TUG protein in skeletal muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Michael G Löffler,Andreas L Birkenfeld,Katerina M Philbrick,Jonathan P Belman,Estifanos N Habtemichael,Carmen J Booth,Carlos M Castorena,Cheol Soo Choi,Francois R Jornayvaz,Brandon M Gassaway,Hui-Young Lee,Gregory D Cartee,William Philbrick,Gerald I Shulman,Varman T Samuel,Jonathan S Bogan
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com