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Rabbit Recombinant Monoclonal PIST antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

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Images

Western blot - Anti-PIST antibody [EPR4080(2)] - BSA and Azide free (AB248513), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4080(2)] - BSA and Azide free (AB248513), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4080(2)] - BSA and Azide free (AB248513), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4080(2)] - BSA and Azide free (AB248513), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4080(2)] - BSA and Azide free (AB248513), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Target data

Function

Plays a role in intracellular protein trafficking and degradation (PubMed:11707463, PubMed:14570915, PubMed:15358775). May regulate CFTR chloride currents and acid-induced ASIC3 currents by modulating cell surface expression of both channels (By similarity). May also regulate the intracellular trafficking of the ADR1B receptor (PubMed:15358775). May play a role in autophagy (By similarity). Together with MARCHF2 mediates the ubiquitination and lysosomal degradation of CFTR (PubMed:23818989). Overexpression results in CFTR intracellular retention and lysosomaldegradation in the lysosomes (PubMed:11707463, PubMed:14570915).

Alternative names

CAL, FIG, GOPC, Golgi-associated PDZ and coiled-coil motif-containing protein, CFTR-associated ligand, Fused in glioblastoma, PDZ protein interacting specifically with TC10, PIST

Recommended products

Rabbit Recombinant Monoclonal PIST antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR4080(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248513 is the carrier-free version of Anti-PIST antibody [EPR4080(2)] ab133472.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PIST also known as PDZ domain-containing protein 8 (GOPC) is a protein with a molecular weight of approximately 55 kDa. This protein is found in multiple tissues with higher expression in the brain and testes. PIST interacts with Golgi membranes and serves as a scaffold in cellular processes by linking transmembrane proteins to different signaling pathways. Its main functional domain the PDZ domain facilitates specific binding to target proteins allowing it to play a versatile role in maintaining cellular functions.

Biological function summary

PIST participates in protein trafficking and sorting. It associates with the complex that regulates vesicular transport ensuring protein delivery to proper cellular locations. In neuronal cells PIST influences the trafficking of receptors like AMPA and NMDA which are important for synaptic function. The involvement of PIST in protein trafficking highlights its significance in cellular organization and communication.

Pathways

PIST engages in interactions within the Wnt and PI3K/Akt signaling pathways. In the Wnt pathway PIST supports the stabilization and trafficking of receptors critical for signal transduction. In the PI3K/Akt pathway it influences cell survival and proliferation by modulating receptor localization and function. Proteins such as Dishevelled and PTEN are often associated with PIST's activity in these pathways highlighting its diverse biological importance.

Associated diseases and disorders

PIST shows connections to conditions like cancer and neurodegenerative disorders. In cancer deregulation of PIST has been observed potentially impacting cell proliferation and survival since it interacts with proteins like EGFR and other growth factor receptors. In neurodegenerative diseases abnormal PIST function may disrupt synaptic signaling linking it to disorders like Alzheimer's through its interactions with synaptic proteins.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-PIST antibody [EPR4080(2)] - BSA and Azide free (ab248513), expandable thumbnail

    Western blot - Anti-PIST antibody [EPR4080(2)] - BSA and Azide free (ab248513)

    This data was developed using Anti-PIST antibody [EPR4080(2)] ab133472, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-PIST antibody [EPR4080(2)] (Anti-PIST antibody [EPR4080(2)] ab133472) at 1/1000 dilution

    Lane 1: Human fetal kidney lysate at 10 µg

    Lane 2: PC-3 cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 51 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4080(2)] - BSA and Azide free (ab248513), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4080(2)] - BSA and Azide free (ab248513)

    This data was developed using Anti-PIST antibody [EPR4080(2)] ab133472, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of PIST in paraffin embedded Human kidney tissue, using Anti-PIST antibody [EPR4080(2)] ab133472 at a dilution of 1/50-1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4080(2)] - BSA and Azide free (ab248513), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4080(2)] - BSA and Azide free (ab248513)

    This data was developed using Anti-PIST antibody [EPR4080(2)] ab133472, the same antibody clone in a different buffer formulation.Anti-PIST antibody [EPR4080(2)] ab133472 showing positive staining in Normal liver tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4080(2)] - BSA and Azide free (ab248513), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4080(2)] - BSA and Azide free (ab248513)

    This data was developed using Anti-PIST antibody [EPR4080(2)] ab133472, the same antibody clone in a different buffer formulation.Anti-PIST antibody [EPR4080(2)] ab133472 showing positive staining in Normal breast tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4080(2)] - BSA and Azide free (ab248513), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIST antibody [EPR4080(2)] - BSA and Azide free (ab248513)

    This data was developed using Anti-PIST antibody [EPR4080(2)] ab133472, the same antibody clone in a different buffer formulation.Anti-PIST antibody [EPR4080(2)] ab133472 showing positive staining in Normal stomach tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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