JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB10623

Anti-Pit1 antibody [2C11]

Be the first to review this product! Submit a review

|

(4 Publications)

Mouse Monoclonal PIT1 antibody. Suitable for WB and reacts with Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Pou1f1 aa 1-150.

View Alternative Names

Ghf-1, Pit-1, Pit1, Pou1f1, Pituitary-specific positive transcription factor 1, PIT-1, Growth hormone factor 1, GHF-1

1 Images
Western blot - Anti-Pit1 antibody [2C11] (AB10623)
  • WB

Unknown

Western blot - Anti-Pit1 antibody [2C11] (AB10623)

At 1 : 40 dilution the western was very good on GH3 nuclear lysate which has an abundant expression of Pit1.

At 1 : 40 dilution the western was very good on GH3 nuclear lysate which has an abundant expression of Pit1.

Lane 1:

Western blot - Anti-Pit1 antibody [2C11] (ab10623)

Lane 1:

Western blot - Anti-Pit1 antibody [5E4] (<a href='/en-us/products/primary-antibodies/pit1-antibody-5e4-ab10545'>ab10545</a>)

Predicted band size: 33 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2C11

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Rat

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Rat Pou1f1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

P10037

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pit1 also known as POU1F1 is a transcription factor with a mass of approximately 33 kDa. This protein is involved in the regulation of pituitary-specific genes and is critical for the development and function of the pituitary gland. It expresses mainly in the anterior pituitary where it activates several genes required for pituitary hormone production. Pit1 plays a role in differentiating somatotrophs lactotrophs and thyrotrophs which produce growth hormone prolactin and thyroid-stimulating hormone respectively.
Biological function summary

The Pit1 protein regulates the transcription of genes related to hormonal production in the anterior pituitary gland. It interacts with other transcription factors to form a complex that ensures the precise regulation of target genes. This regulation is essential for normal growth metabolism and reproductive functions. By controlling the expression of growth hormone thyroid-stimulating hormone and prolactin Pit1 directly affects various physiological processes in the body.

Pathways

Pit1 plays a vital role in the growth hormone (GH) and prolactin signaling pathways. It interacts with various regulatory proteins that modulate gene expression within these pathways. In the GH pathway Pit1 collaborates with proteins like GH-releasing hormone receptor to ensure adequate hormone levels in circulation. Additionally within the prolactin pathway Pit1 ensures the proper function of Jak-STAT signaling important for reproductive and metabolic functions. Its activity impacts important pathway components and further affects energy balance and cell proliferation.

Mutations or alterations in the Pit1 gene can lead to combined pituitary hormone deficiency (CPHD). This condition results in impaired hormone production affecting growth and development. Pit1 interacts with proteins like PROP1 which influences pituitary function and contributes to the pathogenesis of CPHD. Another disorder associated with Pit1 is congenital hypothyroidism where thyroid-stimulating hormone production is hindered impacting metabolism and growth. Understanding the role of Pit1 in these conditions can help in developing targeted therapies to manage hormonal disorders effectively.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.
See full target information Pou1f1
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Endocrinology 160:2673-2691 PubMed31436800

2019

Decreased Activity of the Ghrhr and Gh Promoters Causes Dominantly Inherited GH Deficiency in Humanized GH1 Mouse Models.

Applications

Unspecified application

Species

Unspecified reactive species

Daisuke Ariyasu,Emika Kubo,Daisuke Higa,Shinsuke Shibata,Yutaka Takaoka,Michihiko Sugimoto,Kazunori Imaizumi,Tomonobu Hasegawa,Kimi Araki

The Journal of biological chemistry 285:34718-28 PubMed20807761

2010

MicroRNAs regulate pituitary development, and microRNA 26b specifically targets lymphoid enhancer factor 1 (Lef-1), which modulates pituitary transcription factor 1 (Pit-1) expression.

Applications

IHC-P

Species

Mouse

Zichao Zhang,Sergio Florez,Arthur Gutierrez-Hartmann,James F Martin,Brad A Amendt

Journal of cellular biochemistry 92:664-78 PubMed15211565

2004

Inactivating Pit-1 mutations alter subnuclear dynamics suggesting a protein misfolding and nuclear stress response.

Applications

Unspecified application

Species

Unspecified reactive species

Z Dave Sharp,David L Stenoien,Maureen G Mancini,Ilia I Ouspenski,Michael A Mancini

Journal of cellular biochemistry 72:322-38 PubMed10022514

1999

Subnuclear partitioning and functional regulation of the Pit-1 transcription factor.

Applications

Unspecified application

Species

Unspecified reactive species

M G Mancini,B Liu,Z D Sharp,M A Mancini
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB273048

Anti-Pit1 antibody [EPR23555-203]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pit1 antibody [EPR23555-203] (AB273048)

  • 0
  • 0
Primary Antibodies

AB10545

Anti-Pit1 antibody [5E4]

Immunocytochemistry/ Immunofluorescence - Anti-Pit1 antibody [5E4] (AB10545)

  • 5
  • 1

Complementary Products

Primary Antibodies

AB243028

Anti-Tpit antibody [CL6251]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tpit antibody [CL6251] (AB243028)

  • 0
  • 0
Primary Antibodies

AB217317

Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744]

RabMAb|Recombinant|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Steroidogenic Factor 1/SF-1 antibody [EPR19744] (AB217317)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB134152

Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - C-terminal (AB134152)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com