AB10545

Anti-Pit1 antibody [5E4]

Name: Anti-Pit1 antibody [5E4] (ab10545) | Abcam
Brand: Abcam Limited
SKU: AB10545
Price: 545 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(3 Publications)

Mouse Monoclonal PIT1 antibody. Suitable for IP, WB, ICC/IF and reacts with Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Rat Pou1f1 aa 1-150.

View Alternative Names

Ghf-1, Pit-1, Pit1, Pou1f1, Pituitary-specific positive transcription factor 1, PIT-1, Growth hormone factor 1, GHF-1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Pit1 antibody [5E4] (AB10545)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Pit1 antibody [5E4] (AB10545)

IF staining on rat samples using ab10545 carried out as per the reference (see below) by Zelton Dave Sharp of The University of Texas, San Antonio.

This image is courtesy of Zelton Dave Sharp

PubMed

Immunoprecipitation - Anti-Pit1 antibody [5E4] (AB10545)

Antibody to Pit1 (ab10545) detects a 33 kDa band in IP followed by WB.

Two IgG monoclonal antibodies were used to immunoprecipitate pituitary-derived GH3 cellular proteins, and followed by Western analysis. Whole cell extracts (WCE) and immunoprecipitated fractions contain a prominent 33 kD band representing Pit-1. Nonspecific hybridoma supernatant was used as a negative control. Epitope mapping (data not shown) indicated that both IgG monoclonal antibodies recognize an epitope in the distal N-terminus within the ST activation domain of Pit-1

All lanes:

Immunoprecipitation - Anti-Pit1 antibody [5E4] (ab10545)

Predicted band size: 33 kDa

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Unknown

Western blot - Anti-Pit1 antibody [5E4] (AB10545)

At 1 : 40 dilution the western was very good on GH3 nuclear lysate which has an abundant expression of Pit1. At 1 : 40 dilution the western was very good on GH3 nuclear lysate which has an abundant expression of Pit1.

Lane 1:

Western blot - Anti-Pit1 antibody [2C11] (<a href='/en-us/products/primary-antibodies/pit1-antibody-2c11-ab10623'>ab10623</a>)

Lane 1:

Western blot - Anti-Pit1 antibody [5E4] (ab10545)

Predicted band size: 33 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5E4

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Rat

Applications

WB, IP, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Rat Pou1f1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

P10037

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/40", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Macaque monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Sheep": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pit1 also known as POU1F1 is a transcription factor with a mass of approximately 33 kDa. This protein is involved in the regulation of pituitary-specific genes and is critical for the development and function of the pituitary gland. It expresses mainly in the anterior pituitary where it activates several genes required for pituitary hormone production. Pit1 plays a role in differentiating somatotrophs lactotrophs and thyrotrophs which produce growth hormone prolactin and thyroid-stimulating hormone respectively.

Biological function summary

The Pit1 protein regulates the transcription of genes related to hormonal production in the anterior pituitary gland. It interacts with other transcription factors to form a complex that ensures the precise regulation of target genes. This regulation is essential for normal growth metabolism and reproductive functions. By controlling the expression of growth hormone thyroid-stimulating hormone and prolactin Pit1 directly affects various physiological processes in the body.

Pathways

Pit1 plays a vital role in the growth hormone (GH) and prolactin signaling pathways. It interacts with various regulatory proteins that modulate gene expression within these pathways. In the GH pathway Pit1 collaborates with proteins like GH-releasing hormone receptor to ensure adequate hormone levels in circulation. Additionally within the prolactin pathway Pit1 ensures the proper function of Jak-STAT signaling important for reproductive and metabolic functions. Its activity impacts important pathway components and further affects energy balance and cell proliferation.

Mutations or alterations in the Pit1 gene can lead to combined pituitary hormone deficiency (CPHD). This condition results in impaired hormone production affecting growth and development. Pit1 interacts with proteins like PROP1 which influences pituitary function and contributes to the pathogenesis of CPHD. Another disorder associated with Pit1 is congenital hypothyroidism where thyroid-stimulating hormone production is hindered impacting metabolism and growth. Understanding the role of Pit1 in these conditions can help in developing targeted therapies to manage hormonal disorders effectively.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Activates growth hormone and prolactin genes. Specifically binds to the consensus sequence 5'-TAAAT-3'.

See full target information Pou1f1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 11:4664 PubMed32938919

2020

IRF1-mediated downregulation of PGC1α contributes to cardiorenal syndrome type 4.

Applications

Unspecified application

Species

Unspecified reactive species

Yinghui Huang,Shaobo Wang,Jie Zhou,Yong Liu,Changhong Du,Ke Yang,Xianjin Bi,Mingying Liu,Wenhao Han,Kailong Wang,Jiachuan Xiong,Song Wang,Yue Wang,Ling Nie,Chi Liu,Daohai Zhang,Jun Gu,Chunyu Zeng,Jinghong Zhao

PloS one 12:e0180393 PubMed28686724

2017

Dipeptidyl peptidase-4 inhibitor gemigliptin protects against vascular calcification in an experimental chronic kidney disease and vascular smooth muscle cells.

Applications

Species

Human

Soon-Youn Choi,Hye-Myung Ryu,Eun-Joo Oh,Ji-Young Choi,Jang-Hee Cho,Chan-Duck Kim,Yong-Lim Kim,Sun-Hee Park

Journal of cellular biochemistry 72:322-38 PubMed10022514

1999

Subnuclear partitioning and functional regulation of the Pit-1 transcription factor.

Applications

Unspecified application

Species

Unspecified reactive species

M G Mancini,B Liu,Z D Sharp,M A Mancini

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Pit1 antibody [5E4]

Immunocytochemistry/ Immunofluorescence - Anti-Pit1 antibody [5E4] (AB10545)

Immunoprecipitation - Anti-Pit1 antibody [5E4] (AB10545)

Western blot - Anti-Pit1 antibody [5E4] (AB10545)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purity

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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