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Rabbit Polyclonal PIT1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human POU1F1 aa 1-100.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human POU1F1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P28069
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested
Mouse
Predicted
Rat
Predicted
Cow
Predicted
Dog
Predicted
Rhesus monkey
Predicted
Sheep
Predicted
Turkey
Predicted

Tested
Tested

Species

Human

Dilution info

1/1000.00000 - 1/2500.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat, Sheep, Cow, Dog, Turkey, Rhesus monkey

Dilution info

-

Notes

-

Target data

Function

Transcription factor involved in the specification of the lactotrope, somatotrope, and thyrotrope phenotypes in the developing anterior pituitary. Specifically binds to the consensus sequence 5'-TAAAT-3'. Activates growth hormone and prolactin genes (PubMed:22010633, PubMed:26612202).

Alternative names

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Rabbit Polyclonal PIT1 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human POU1F1 aa 1-100.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human POU1F1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P28069
Purification technique

Affinity purification Immunogen

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

The Pit1 protein regulates the transcription of genes related to hormonal production in the anterior pituitary gland. It interacts with other transcription factors to form a complex that ensures the precise regulation of target genes. This regulation is essential for normal growth metabolism and reproductive functions. By controlling the expression of growth hormone thyroid-stimulating hormone and prolactin Pit1 directly affects various physiological processes in the body.

Activity summary

Pit1 also known as POU1F1 is a transcription factor with a mass of approximately 33 kDa. This protein is involved in the regulation of pituitary-specific genes and is critical for the development and function of the pituitary gland. It expresses mainly in the anterior pituitary where it activates several genes required for pituitary hormone production. Pit1 plays a role in differentiating somatotrophs lactotrophs and thyrotrophs which produce growth hormone prolactin and thyroid-stimulating hormone respectively.

Pathways

Pit1 plays a vital role in the growth hormone (GH) and prolactin signaling pathways. It interacts with various regulatory proteins that modulate gene expression within these pathways. In the GH pathway Pit1 collaborates with proteins like GH-releasing hormone receptor to ensure adequate hormone levels in circulation. Additionally within the prolactin pathway Pit1 ensures the proper function of Jak-STAT signaling important for reproductive and metabolic functions. Its activity impacts important pathway components and further affects energy balance and cell proliferation.

Associated diseases and disorders

Mutations or alterations in the Pit1 gene can lead to combined pituitary hormone deficiency (CPHD). This condition results in impaired hormone production affecting growth and development. Pit1 interacts with proteins like PROP1 which influences pituitary function and contributes to the pathogenesis of CPHD. Another disorder associated with Pit1 is congenital hypothyroidism where thyroid-stimulating hormone production is hindered impacting metabolism and growth. Understanding the role of Pit1 in these conditions can help in developing targeted therapies to manage hormonal disorders effectively.

Product promise

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4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pit1 antibody (ab272639), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pit1 antibody (ab272639)

    Paraffin-embedded formalin-fixed Human pituitary tissue stained for Pit1 using ab272639 at 1/2500 in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pit1 antibody (ab272639), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pit1 antibody (ab272639)

    Paraffin-embedded formalin-fixed Human tonsil tissue stained for Pit1 using ab272639 at 1/2500 in immunohistochemical analysis. Shows no expression as expected.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pit1 antibody (ab272639), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pit1 antibody (ab272639)

    Paraffin-embedded formalin-fixed Human pancreas tissue stained for Pit1 using ab272639 at 1/2500 in immunohistochemical analysis. Shows no expression as expected.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pit1 antibody (ab272639), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pit1 antibody (ab272639)

    Paraffin-embedded formalin-fixed Human liver tissue stained for Pit1 using ab272639 at 1/2500 in immunohistochemical analysis. Shows no expression as expected.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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