Anti-Pit1 antibody [EPR28191-52] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pit1 antibody [EPR28191-52] - BSA and Azide free (AB313643)

This data was developed using ab313642, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Pit1 with ab313642 at 1/100 (5.25 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on human liver.The section was incubated with ab313642 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pit1 antibody [EPR28191-52] - BSA and Azide free (AB313643)

This data was developed using ab313642, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human pituitary tissue labeling Pit1 with ab313642 at 1/100 (5.25 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human pituitary (PMID : 2902927, 27351100).The section was incubated with ab313642 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pit1 antibody [EPR28191-52] - BSA and Azide free (AB313643)

This data was developed using ab313642, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Pit1 with ab313642 at 1/500 (1.05 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on mouse liver.The section was incubated with ab313642 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pit1 antibody [EPR28191-52] - BSA and Azide free (AB313643)

This data was developed using ab313642, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Pit1 with ab313642 at 1/500 (1.05 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : no staining on rat liver.The section was incubated with ab313642 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pit1 antibody [EPR28191-52] - BSA and Azide free (AB313643)

This data was developed using ab313642, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse pituitary tissue labeling Pit1 with ab313642 at 1/500 (1.05 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse pituitary.The section was incubated with ab313642 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pit1 antibody [EPR28191-52] - BSA and Azide free (AB313643)

This data was developed using ab313642, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat pituitary tissue labeling Pit1 with ab313642 at 1/500 (1.05 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat pituitary.The section was incubated with ab313642 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-Pit1 antibody [EPR28191-52] - BSA and Azide free (AB313643)

This data was developed using ab313642, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : cerebellum (PMID : 2902927), liver (PMID : 2902927), kidney (PMID : 2902927), spleen. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 33927188, 16968807). The identity of the higher MW band at approximately 70 kDa is unknown. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds.

All lanes:

Western blot - Anti-Pit1 antibody [EPR28191-52] (<a href='/en-us/products/primary-antibodies/pit1-antibody-epr28191-52-ab313642'>ab313642</a>) at 1/1000 dilution

Lane 1:

Human pituitary tissue lysate at 20 µg

Lane 2:

Human cerebellum tissue lysate at 20 µg

Lane 3:

Human liver tissue lysate at 20 µg

Lane 4:

Human kidney tissue lysate at 20 µg

Lane 5:

Human spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 33 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Pit1 antibody [EPR28191-52] - BSA and Azide free (AB313643)

This data was developed using ab313642, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : liver, skeletal muscle (PMID : 2902927). The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 33927188, 16968807). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-Pit1 antibody [EPR28191-52] (<a href='/en-us/products/primary-antibodies/pit1-antibody-epr28191-52-ab313642'>ab313642</a>) at 1/1000 dilution

Lane 1:

Rat pituitary tissue lysate at 20 µg

Lane 2:

Rat liver tissue lysate at 20 µg

Lane 3:

Rat skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 33 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Pit1 antibody [EPR28191-52] - BSA and Azide free (AB313643)

This data was developed using ab313642, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : brain (PMID : 2902927), kidney (PMID : 2902927), heart, spleen. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 33927188, 16968807). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 180 seconds

All lanes:

Western blot - Anti-Pit1 antibody [EPR28191-52] (<a href='/en-us/products/primary-antibodies/pit1-antibody-epr28191-52-ab313642'>ab313642</a>) at 1/1000 dilution

Lane 1:

Mouse pituitary tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Mouse heart tissue lysate at 20 µg

Lane 4:

Mouse kidney tissue lysate at 20 µg

Lane 5:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 33 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Pit1 antibody [EPR28191-52] - BSA and Azide free (AB313643)

This data was developed using ab313642, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti-His antibody (ab213204) staining at 1/5000 dilution. Anti-H3 antibody (ab176842) loading control staining at 1/100000 dilution. Exposure time : 6 seconds

All lanes:

Western blot - Anti-Pit1 antibody [EPR28191-52] (<a href='/en-us/products/primary-antibodies/pit1-antibody-epr28191-52-ab313642'>ab313642</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containi a His-tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human Pit1 expression vector containi a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 33 kDa

false

Exposure time: 6s