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Rabbit Recombinant Monoclonal PITPnm 3 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITPnm 3 antibody [EPR28185-157] (AB323452), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITPnm 3 antibody [EPR28185-157] (AB323452), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITPnm 3 antibody [EPR28185-157] (AB323452), expandable thumbnail
  • Western blot - Anti-PITPnm 3 antibody [EPR28185-157] (AB323452), expandable thumbnail
  • Western blot - Anti-PITPnm 3 antibody [EPR28185-157] (AB323452), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Not recommended
Tested
Not recommended
Mouse
Tested
Tested
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Target data

Function

Catalyzes the transfer of phosphatidylinositol and phosphatidylcholine between membranes (in vitro) (By similarity). Binds calcium ions.

Additional Targets

Pitpnm3

Alternative names

NIR1, PITPNM3, Membrane-associated phosphatidylinositol transfer protein 3, Pyk2 N-terminal domain-interacting receptor 1, PITPnm 3, NIR-1

Rabbit Recombinant Monoclonal PITPnm 3 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28185-157
Purification technique
Affinity purification Protein A
Specificity

Not suitable for human IHC

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITPnm 3 antibody [EPR28185-157] (ab323452), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITPnm 3 antibody [EPR28185-157] (ab323452)

    Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling PITPnm 3 with ab323452 at 1/100 (4.9 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression tissue: No staining on mouse liver (PMID: 10022914).

    The section was incubated with ab323452 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITPnm 3 antibody [EPR28185-157] (ab323452), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITPnm 3 antibody [EPR28185-157] (ab323452)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling PITPnm 3 with ab323452 at 1/100 (4.9 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse cerebellum.

    The section was incubated with ab323452 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITPnm 3 antibody [EPR28185-157] (ab323452), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITPnm 3 antibody [EPR28185-157] (ab323452)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling PITPnm 3 with ab323452 at 1/100 (4.9 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse cerebrum.

    The section was incubated with ab323452 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Western blot - Anti-PITPnm 3 antibody [EPR28185-157] (ab323452), expandable thumbnail

    Western blot - Anti-PITPnm 3 antibody [EPR28185-157] (ab323452)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: pancreas, liver (PMID: 10022914), A20.

    The identity of the bands lower than 60 kDa are unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-2: 37 seconds; Lanes 3-5: 6 seconds.

    All lanes: Western blot - Anti-PITPnm 3 antibody [EPR28185-157] (ab323452) at 1/1000 dilution

    Lane 1: mIMCD3 (mouse inner medlary collecting duct epithelial cell) whole cell lysate at 20 µg

    Lane 2: A20 (mouse reticulum sarcoma b lymphocyte) whole cell at 20 µg

    Lane 3: Mouse brain tissue lysate at 20 µg

    Lane 4: Mouse liver tissue lysate at 20 µg

    Lane 5: Mouse pancreas tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 106 kDa, 70 kDa, 36 kDa

  • Western blot - Anti-PITPnm 3 antibody [EPR28185-157] (ab323452), expandable thumbnail

    Western blot - Anti-PITPnm 3 antibody [EPR28185-157] (ab323452)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: liver (PMID: 10022914).

    The identity of the lower MW band at approximately 37 kDa (in lane 1) is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-PITPnm 3 antibody [EPR28185-157] (ab323452) at 1/1000 dilution

    Lane 1: Human brain tissue lysate at 20 µg

    Lane 2: Human liver tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 106 kDa, 70 kDa, 36 kDa

    Exposure time: 125s

  • Western blot - Anti-PITPnm 3 antibody [EPR28185-157] (ab323452), expandable thumbnail

    Western blot - Anti-PITPnm 3 antibody [EPR28185-157] (ab323452)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: THP-1, Huh7 (PMID: 26449829).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-PITPnm 3 antibody [EPR28185-157] (ab323452) at 1/1000 dilution

    Lane 1: HCC1937 (human mammary gland epithelial cell) whole cell lysate at 20 µg

    Lane 2: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Lane 3: Huh7 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 106 kDa, 70 kDa, 36 kDa

    Exposure time: 37s

  • Western blot - Anti-PITPnm 3 antibody [EPR28185-157] (ab323452), expandable thumbnail

    Western blot - Anti-PITPnm 3 antibody [EPR28185-157] (ab323452)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 32641093).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-PITPnm 3 antibody [EPR28185-157] (ab323452) at 1/1000 dilution

    Lane 1: SK-OV-3 (human ovarian cancer epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: SK-OV-3 transfected with siRNA specifically targeting PITPnm 3 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 106 kDa, 70 kDa, 36 kDa

    Exposure time: 10s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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