Anti-PITPnm 3 antibody [EPR28185-157]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITPnm 3 antibody [EPR28185-157] (AB323452)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling PITPnm 3 with ab323452 at 1/100 (4.9 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse cerebrum.
The section was incubated with ab323452 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITPnm 3 antibody [EPR28185-157] (AB323452)

Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling PITPnm 3 with ab323452 at 1/100 (4.9 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse cerebellum.
The section was incubated with ab323452 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• WB

Supplier Data

Western blot - Anti-PITPnm 3 antibody [EPR28185-157] (AB323452)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : liver (PMID : 10022914).

The identity of the lower MW band at approximately 37 kDa (in lane 1) is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-PITPnm 3 antibody [EPR28185-157] (ab323452) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 106 kDa,70 kDa,36 kDa

false

Exposure time: 125s

• WB

Supplier Data

Western blot - Anti-PITPnm 3 antibody [EPR28185-157] (AB323452)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 32641093).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-PITPnm 3 antibody [EPR28185-157] (ab323452) at 1/1000 dilution

Lane 1:

SK-OV-3 (human ovarian cancer epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

SK-OV-3 transfected with siRNA specifically targeting PITPnm 3 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 106 kDa,70 kDa,36 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Anti-PITPnm 3 antibody [EPR28185-157] (AB323452)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : THP-1, Huh7 (PMID : 26449829).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-PITPnm 3 antibody [EPR28185-157] (ab323452) at 1/1000 dilution

Lane 1:

HCC1937 (human mammary gland epithelial cell) whole cell lysate at 20 µg

Lane 2:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 3:

Huh7 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 106 kDa,70 kDa,36 kDa

false

Exposure time: 37s

• WB

Supplier Data

Western blot - Anti-PITPnm 3 antibody [EPR28185-157] (AB323452)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : pancreas, liver (PMID : 10022914), A20.

The identity of the bands lower than 60 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-2 : 37 seconds; Lanes 3-5 : 6 seconds.

All lanes:

Western blot - Anti-PITPnm 3 antibody [EPR28185-157] (ab323452) at 1/1000 dilution

Lane 1:

mIMCD3 (mouse inner medlary collecting duct epithelial cell) whole cell lysate at 20 µg

Lane 2:

A20 (mouse reticulum sarcoma b lymphocyte) whole cell at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Lane 4:

Mouse liver tissue lysate at 20 µg

Lane 5:

Mouse pancreas tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 106 kDa,70 kDa,36 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITPnm 3 antibody [EPR28185-157] (AB323452)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling PITPnm 3 with ab323452 at 1/100 (4.9 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : No staining on mouse liver (PMID : 10022914).
The section was incubated with ab323452 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins