AB70273

Anti-PITX1/BFT antibody

Name: Anti-PITX1/BFT antibody (ab70273) | Abcam
Brand: Abcam Limited
SKU: AB70273
Price: 525 USD
Availability: InStock

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(9 Publications)

Rabbit Polyclonal PITX1/BFT antibody. Suitable for IP, WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human PITX1.

View Alternative Names

BFT, PTX1, PITX1, Pituitary homeobox 1, Hindlimb-expressed homeobox protein backfoot, Homeobox protein PITX1, Paired-like homeodomain transcription factor 1

2 Images

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Immunoprecipitation - Anti-PITX1/BFT antibody (AB70273)

HeLa whole cell lysate (1 mg) was immunoprecipitated using ab70273 at 3 µg/mg lysate (lane 1) or an IgG control (lane 2). One quarter of the total IP reaction was loaded on to a gel and analysed by Western blot using ab70273 at 1 µg/ml. Bands were detected using chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-PITX1/BFT antibody (ab70273)

Predicted band size: 34 kDa

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Western blot - Anti-PITX1/BFT antibody (AB70273)

All lanes:

Western blot - Anti-PITX1/BFT antibody (ab70273) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 34 kDa

Observed band size: 40 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human PITX1. The exact immunogen used to generate this antibody is proprietary information.

P78337

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1-4 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2500 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PITX1 gene also known as BFT or BFT NZ encodes the Pituitary Homeobox 1 protein. This protein is a homeodomain transcription factor and plays an important role in regulating gene expression by binding to specific DNA sequences. PITX1 has a molecular mass of approximately 33 kDa. It is primarily expressed in tissues like the pituitary gland lower limbs and jaw. The expression in these specific areas hints at its importance in the developmental processes that occur during embryogenesis.

Biological function summary

The PITX1 protein functions as part of the regulation of limb and craniofacial development. It is not part of a larger protein complex but works independently to influence the differentiation and development of specific cell types. In the embryonic stages PITX1 directs the growth and formation of lower limb structures and mutations in this gene can lead to limb malformations. The protein also has roles in craniofacial development highlighting its multifaceted biological functions.

Pathways

PITX1 is involved in the Wnt signaling pathway and the Hedgehog pathway both essential for proper developmental processes. In the Wnt signaling pathway PITX1 interacts with other proteins such as β-catenin to modulate gene expression necessary for limb development. In the Hedgehog pathway PITX1 influences how cells respond to Hedgehog signals affecting cell growth and specialization. The coordinated role of PITX1 in these pathways supports its critical involvement in developmental regulation.

PITX1 has connections to clubfoot and Liebenberg syndrome. Clubfoot a congenital deformity of the foot often links to mutations or alterations in PITX1 expression affecting limb development. Moreover Liebenberg syndrome suggests altered expression or function of PITX1 leading to misregulated limb and joint formation. In both diseases associations with proteins like β-catenin and hedgehog proteins illustrate how changes in PITX1 can result in significant phenotypic abnormalities reflecting its importance in both normal and pathological conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sequence-specific transcription factor that binds gene promoters and activates their transcription. May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb.

See full target information PITX1

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Cancers 14: PubMed35267575

2022

PITX1 Is a Regulator of TERT Expression in Prostate Cancer with Prognostic Power.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra M Poos,Cornelia Schroeder,Neeraja Jaishankar,Daniela Röll,Marcus Oswald,Jan Meiners,Delia M Braun,Caroline Knotz,Lukas Frank,Manuel Gunkel,Roman Spilger,Thomas Wollmann,Adam Polonski,Georgia Makrypidi-Fraune,Christoph Fraune,Markus Graefen,Inn Chung,Alexander Stenzel,Holger Erfle,Karl Rohr,Aria Baniahmad,Guido Sauter,Karsten Rippe,Ronald Simon,Rainer Koenig

Scientific reports 11:18405 PubMed34526609

2021

PITX1 inhibits the growth and proliferation of melanoma cells through regulation of SOX family genes.

Applications

Unspecified application

Species

Unspecified reactive species

Takahito Ohira,Suguru Nakagawa,Jumpei Takeshita,Hiroyuki Aburatani,Hiroyuki Kugoh

The Journal of biological chemistry 295:13677-13690 PubMed32759168

2020

The transcription factor PITX1 drives astrocyte differentiation by regulating the SOX9 gene.

Applications

Unspecified application

Species

Unspecified reactive species

Jeong Su Byun,Mihee Oh,Seonha Lee,Jung-Eun Gil,Yeajin Mo,Bonsu Ku,Won-Kon Kim,Kyoung-Jin Oh,Eun-Woo Lee,Kwang-Hee Bae,Sang Chul Lee,Baek-Soo Han

European journal of pharmacology 863:172643 PubMed31493405

2019

Circular RNA circ-PITX1 promotes the progression of glioblastoma by acting as a competing endogenous RNA to regulate miR-379-5p/MAP3K2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xinwen Lv,Meihua Wang,Jingli Qiang,Shiwen Guo

PloS one 14:e0217605 PubMed31404068

2019

PITX1 protein interacts with ZCCHC10 to regulate hTERT mRNA transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Takahito Ohira,Hirotada Kojima,Yuko Kuroda,Sayaka Aoki,Daigo Inaoka,Mitsuhiko Osaki,Hideki Wanibuchi,Futoshi Okada,Mitsuo Oshimura,Hiroyuki Kugoh

PloS one 11:e0165951 PubMed27802335

2016

E2F1 and TFDP1 Regulate PITX1 Expression in Normal and Osteoarthritic Articular Chondrocytes.

Applications

Species

Human

Martin Pellicelli,Cynthia Picard,DaShen Wang,Patrick Lavigne,Alain Moreau

Scientific reports 6:35308 PubMed27752061

2016

Protein tyrosine phosphatase 1B targets PITX1/p120RasGAP thus showing therapeutic potential in colorectal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hao-Wei Teng,Man-Hsin Hung,Li-Ju Chen,Mao-Ju Chang,Feng-Shu Hsieh,Ming-Hsien Tsai,Jui-Wen Huang,Chih-Lung Lin,Hsiang-Wen Tseng,Zong-Keng Kuo,Jeng-Kai Jiang,Shung-Haur Yang,Chung-Wai Shiau,Kuen-Feng Chen

Nucleic acids research 42:3346-61 PubMed24369427

2013

The RNA helicase RHAU (DHX36) suppresses expression of the transcription factor PITX1.

Applications

Unspecified application

Species

Human

Evan P Booy,Ryan Howard,Oksana Marushchak,Emmanuel O Ariyo,Markus Meier,Stefanie K Novakowski,Soumya R Deo,Edis Dzananovic,Jörg Stetefeld,Sean A McKenna

Arthritis and rheumatism 65:993-1003 PubMed23310948

2013

Nuclear accumulation of prohibitin 1 in osteoarthritic chondrocytes down-regulates PITX1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Cynthia Picard,Martin Pellicelli,Maryam Taheri,Jean-Francois Lavoie,Roxanne Doucet,DaShen Wang,Lauriane Bernard,Saadallah Bouhanik,Patrick Lavigne,Alain Moreau

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PITX1/BFT antibody

Immunoprecipitation - Anti-PITX1/BFT antibody (AB70273)

Western blot - Anti-PITX1/BFT antibody (AB70273)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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