Rabbit Recombinant Monoclonal PITX2/RGS antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 2 publications.
Images
![Western blot - Anti-PITX2/RGS antibody [EPR22417] (AB221142), expandable thumbnail](https://content.abcam.com/products/images/pitx2-rgs-antibody-epr22417-ab221142--western-blot-img108463.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PITX2/RGS antibody [EPR22417] (AB221142)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (AB221142), expandable thumbnail](https://content.abcam.com/products/images/pitx2-rgs-antibody-epr22417-ab221142--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127825.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (AB221142)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (AB221142), expandable thumbnail](https://content.abcam.com/products/images/pitx2-rgs-antibody-epr22417-ab221142--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img59268.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (AB221142)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (AB221142), expandable thumbnail](https://content.abcam.com/products/images/pitx2-rgs-antibody-epr22417-ab221142--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120379.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (AB221142)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (AB221142), expandable thumbnail](https://content.abcam.com/products/images/pitx2-rgs-antibody-epr22417-ab221142--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img121734.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (AB221142)")
Publications
Filter by
- Integrative zoology 18:543-5512022Pitx2 suppression at meiotic stages associates with seasonal inhibition of testis development in Rattus norvegicus caraco.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin Tian,Xixuan Li,Yaqi Ying,Lewen Wang,Yanting Qiao,Dawei Wang,Ying Song,Ning Li,Xiaohui LiuPubMed 35639924
- Frontiers in cell and developmental biology 10:7778872022Deficient Mice Recapitulate Craniofacial Phenotype and Reveal Developmental Basis of -Related Frontonasal Dysplasia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaul P R Iyyanar,Zhaoming Wu,Yu Lan,Yueh-Chiang Hu,Rulang JiangPubMed 35127681
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-Fr | IHC-P | |
|---|---|---|---|---|
| Mouse | Not recommended | Tested | Not recommended | Tested |
| Rat | Not recommended | Tested | Not recommended | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Rat | Dilution info 1/1000 | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
May play a role in myoblast differentiation. When unphosphorylated, associates with an ELAVL1-containing complex, which stabilizes cyclin mRNA and ensuring cell proliferation. Phosphorylation by AKT2 impairs this association, leading to CCND1 mRNA destabilization and progression towards differentiation. Isoform PTX2C. Involved in the establishment of left-right asymmetry in the developing embryo.
Alternative names
ARP1, RGS, RIEG, RIEG1, PITX2, Pituitary homeobox 2, ALL1-responsive protein ARP1, Homeobox protein PITX2, Paired-like homeodomain transcription factor 2, RIEG bicoid-related homeobox transcription factor, Solurshin
Recommended products
Rabbit Recombinant Monoclonal PITX2/RGS antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR22417
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The PITX2 protein also known as paired-like homeodomain 2 plays a critical role in transcription regulation influencing gene expression. As a transcription factor PITX2 belongs to the bicoid-like homeobox transcription factor family and weighs approximately 32 kDa. It is expressed in several tissues including the eye brain heart and pituitary gland. This expression pattern suggests its involvement in various developmental processes and functions.
- Biological function summary
PITX2 influences the development of several organs and is pivotal in left-right asymmetry during embryogenesis. By regulating gene expression it helps form structural components while ensuring proper tissue differentiation. PITX2 acts as a part of a transcriptional complex that facilitates its role in these developmental pathways. It interacts with other transcription factors and coactivators fine-tuning the transcriptional activities that guide embryonic organ development.
- Pathways
PITX2 is an essential component of the Wnt signaling and heart development pathways. Within these pathways PITX2 regulates genes that participate in important cellular processes affecting cell fate and organ positioning. The protein interacts with other pathway members including β-catenin which mediates its transcriptional activities. These interactions allow it to influence the signaling cascades dictating correct cellular responses necessary for development.
- Associated diseases and disorders
PITX2 is associated with Axenfeld-Rieger syndrome and atrial fibrillation. Mutations or dysregulation of PITX2 can cause improper development of eye and craniofacial structures leading to Axenfeld-Rieger syndrome where it may act in concert with FOXC1 another transcription factor. Additionally abnormal PITX2 function links to atrial fibrillation where it affects the rhythmic patterns of the heart. This connection to cardiac regulation highlights its importance in maintaining normal physiological functions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-PITX2/RGS antibody [EPR22417] (ab221142), expandable thumbnail](https://content.abcam.com/products/images/pitx2-rgs-antibody-epr22417-ab221142--western-blot-img108463.jpg "Western blot - Anti-PITX2/RGS antibody [EPR22417] (ab221142)")
Western blot - Anti-PITX2/RGS antibody [EPR22417] (ab221142)
Blovking/Diluting buffer and concentration: 5% NFDM/TBST
These multiple bands observed in pituitary represent different PITX2/RGS isoforms (PITX2A, PITX2B, PITX2C, PITX2D).
All lanes: Western blot - Anti-PITX2/RGS antibody [EPR22417] (ab221142) at 1/1000 dilution
Lane 1: Mouse pituitary gland tissue at 20 µg
Lane 2: Rat pituitary gland tissue at 20 µg
Lane 3: Mouse skeletal Muscle tissue lysate at 20 µg
Lane 4: Rat skeletal muscle tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 30-38 kDa
Exposure time: 3min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (ab221142), expandable thumbnail](https://content.abcam.com/products/images/pitx2-rgs-antibody-epr22417-ab221142--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127825.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (ab221142)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (ab221142)
Immunohistochemical analysis of paraffin-embedded mouse pituitary gland tissue labeling PITX2/RGS with ab221142 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Nuclear staining on mouse pituitary (PMID: 15761027, PMID: 16859901, PMID: 11807026) is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (ab221142), expandable thumbnail](https://content.abcam.com/products/images/pitx2-rgs-antibody-epr22417-ab221142--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img59268.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (ab221142)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (ab221142)
Immunohistochemical analysis of paraffin-embedded rat pituitary gland tissue labeling PITX2/RGS with ab221142 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Nuclear staining on rat pituitary (PMID: 15761027, PMID: 16859901, PMID: 11807026) is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (ab221142), expandable thumbnail](https://content.abcam.com/products/images/pitx2-rgs-antibody-epr22417-ab221142--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120379.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (ab221142)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (ab221142)
Negative control.Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling PITX2/RGS with ab221142 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Negative control, no staining on rat liver (PMID: 11157981) is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (ab221142), expandable thumbnail](https://content.abcam.com/products/images/pitx2-rgs-antibody-epr22417-ab221142--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img121734.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (ab221142)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (ab221142)
Negative control.Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling PITX2/RGS with ab221142 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Negative control, no staining on mouse spleen (PMID: 11157981) is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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