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Rabbit Recombinant Monoclonal PITX2/RGS antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 2 publications.

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Images

Western blot - Anti-PITX2/RGS antibody [EPR22417] (AB221142), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (AB221142), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (AB221142), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (AB221142), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (AB221142), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-FrIHC-P
Mouse
Not recommended
Tested
Not recommended
Tested
Rat
Not recommended
Tested
Not recommended
Tested

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/2000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Controls cell proliferation in a tissue-specific manner and is involved in morphogenesis. During embryonic development, exerts a role in the expansion of muscle progenitors. May play a role in the proper localization of asymmetric organs such as the heart and stomach. Isoform PTX2C is involved in left-right asymmetry the developing embryo (By similarity).

Alternative names

Pituitary homeobox 2, ALL1-responsive protein ARP1, Homeobox protein PITX2, Paired-like homeodomain transcription factor 2, RIEG bicoid-related homeobox transcription factor, Solurshin, RIEG, RGS, RIEG1, ARP1, PITX2

Recommended products

Rabbit Recombinant Monoclonal PITX2/RGS antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 2 publications.

Alternative names

Pituitary homeobox 2, ALL1-responsive protein ARP1, Homeobox protein PITX2, Paired-like homeodomain transcription factor 2, RIEG bicoid-related homeobox transcription factor, Solurshin, RIEG, RGS, RIEG1, ARP1, PITX2

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR22417

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The PITX2 protein also known as paired-like homeodomain 2 plays a critical role in transcription regulation influencing gene expression. As a transcription factor PITX2 belongs to the bicoid-like homeobox transcription factor family and weighs approximately 32 kDa. It is expressed in several tissues including the eye brain heart and pituitary gland. This expression pattern suggests its involvement in various developmental processes and functions.

Biological function summary

PITX2 influences the development of several organs and is pivotal in left-right asymmetry during embryogenesis. By regulating gene expression it helps form structural components while ensuring proper tissue differentiation. PITX2 acts as a part of a transcriptional complex that facilitates its role in these developmental pathways. It interacts with other transcription factors and coactivators fine-tuning the transcriptional activities that guide embryonic organ development.

Pathways

PITX2 is an essential component of the Wnt signaling and heart development pathways. Within these pathways PITX2 regulates genes that participate in important cellular processes affecting cell fate and organ positioning. The protein interacts with other pathway members including β-catenin which mediates its transcriptional activities. These interactions allow it to influence the signaling cascades dictating correct cellular responses necessary for development.

Associated diseases and disorders

PITX2 is associated with Axenfeld-Rieger syndrome and atrial fibrillation. Mutations or dysregulation of PITX2 can cause improper development of eye and craniofacial structures leading to Axenfeld-Rieger syndrome where it may act in concert with FOXC1 another transcription factor. Additionally abnormal PITX2 function links to atrial fibrillation where it affects the rhythmic patterns of the heart. This connection to cardiac regulation highlights its importance in maintaining normal physiological functions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-PITX2/RGS antibody [EPR22417] (ab221142), expandable thumbnail

    Western blot - Anti-PITX2/RGS antibody [EPR22417] (ab221142)

    Blovking/Diluting buffer and concentration: 5% NFDM/TBST

    These multiple bands observed in pituitary represent different PITX2/RGS isoforms (PITX2A, PITX2B, PITX2C, PITX2D).

    All lanes: Western blot - Anti-PITX2/RGS antibody [EPR22417] (ab221142) at 1/1000 dilution

    Lane 1: Mouse pituitary gland tissue at 20 µg

    Lane 2: Rat pituitary gland tissue at 20 µg

    Lane 3: Mouse skeletal Muscle tissue lysate at 20 µg

    Lane 4: Rat skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 35 kDa

    Observed band size: 30-38 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (ab221142), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (ab221142)

    Immunohistochemical analysis of paraffin-embedded mouse pituitary gland tissue labeling PITX2/RGS with ab221142 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Nuclear staining on mouse pituitary (PMID: 15761027, PMID: 16859901, PMID: 11807026) is observed. Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (ab221142), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (ab221142)

    Immunohistochemical analysis of paraffin-embedded rat pituitary gland tissue labeling PITX2/RGS with ab221142 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Nuclear staining on rat pituitary (PMID: 15761027, PMID: 16859901, PMID: 11807026) is observed. Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (ab221142), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (ab221142)

    Negative control.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling PITX2/RGS with ab221142 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Negative control, no staining on rat liver (PMID: 11157981) is observed. Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (ab221142), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PITX2/RGS antibody [EPR22417] (ab221142)

    Negative control.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling PITX2/RGS with ab221142 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Negative control, no staining on mouse spleen (PMID: 11157981) is observed. Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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