AB106827

Anti-PITX3/PTX3 antibody

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(2 Publications)

Goat Polyclonal PITX3/PTX3 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PITX3 aa 1-50.

View Alternative Names

PTX3, PITX3, Pituitary homeobox 3, Homeobox protein PITX3, Paired-like homeodomain transcription factor 3

1 Images

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Western blot - Anti-PITX3/PTX3 antibody (AB106827)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-PITX3/PTX3 antibody (ab106827) at 1 µg/mL

All lanes:

Human cerebellum lysate in RIPA buffer at 35 µg

Predicted band size: 32 kDa

Observed band size: 40 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human PITX3 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

O75364

Specificity

ab106827 is not expected to cross-react with PITX1 and PITX2.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab106827 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PITX3 also known as paired-like homeodomain transcription factor 3 is a transcription factor that plays a role in gene expression regulation. It has a mass of approximately 32 kDa. PITX3 is predominantly found in the eye lens and dopaminergic neurons specifically in the substantia nigra. This region of the brain is vital for motor control. PITX3 expression is important in ocular development and maintaining cellular function in these regions.

Biological function summary

The PITX3 protein regulates critical processes in ocular development and survival of dopaminergic neurons. It is not part of a complex itself but works closely with other regulatory factors influencing various genes involved in neuron differentiation and eye structures. PITX3's activity in these biological systems is fundamental to proper development and cellular homeostasis.

Pathways

PITX3 plays a significant role in the development and function of dopaminergic neurons of the substantia nigra as well as in the formation of eye tissues. It is involved in pathways like Wnt and dopamine synthesis which are critical for neuronal function and vision development. PITX3 interacts with proteins such as Nurr1 in these pathways contributing to neuron survival and differentiation. The balance of these processes directly impacts the development and function of target tissues.

PITX3 mutations are closely associated with ocular diseases like congenital cataracts and neurodegenerative disorders such as Parkinson's disease. In the context of Parkinson's disease PITX3's role alongside proteins like alpha-synuclein is critical; dysregulation in their interaction can lead to dopaminergic neuron degeneration. Understanding PITX3 interactions with other proteins helps illuminate the molecular basis of these conditions offering potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. In addition to its importance during development, it also has roles in the long-term survival and maintenance of the mdDA neurons. Activates NR4A2/NURR1-mediated transcription of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. Acts by decreasing the interaction of NR4A2/NURR1 with the corepressor NCOR2/SMRT which acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state. Essential for the normal lens development and differentiation. Plays a critical role in the maintenance of mitotic activity of lens epithelial cells, fiber cell differentiation and in the control of the temporal and spatial activation of fiber cell-specific crystallins. Positively regulates FOXE3 expression and negatively regulates PROX1 in the anterior lens epithelium, preventing activation of CDKN1B/P27Kip1 and CDKN1C/P57Kip2 and thus maintains lens epithelial cells in cell cycle (By similarity).

See full target information PITX3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cancer immunology, immunotherapy : CII 73:10 PubMed38231444

2024

Rescue of NLRC5 expression restores antigen processing machinery in head and neck cancer cells lacking functional STAT1 and p53.

Applications

Unspecified application

Species

Unspecified reactive species

Brendan L C Kinney,Sreenivasulu Gunti,Vikash Kansal,Connor J Parrish,Nabil F Saba,Yong Teng,Mary Katherine Henry,Fang-Yi Su,Gabriel A Kwong,Nicole C Schmitt

The ocular surface 22:190-203 PubMed34425299

2021

Impaired GSH biosynthesis disrupts eye development, lens morphogenesis and PAX6 function.

Applications

Unspecified application

Species

Unspecified reactive species

Brian Thompson,Ying Chen,Emily A Davidson,Rolando Garcia-Milian,Jaya Prakash Golla,Nicholas Apostolopoulos,David J Orlicky,Kevin Schey,David C Thompson,Vasilis Vasiliou

View all publications

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Product promise

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Anti-PITX3/PTX3 antibody

Western blot - Anti-PITX3/PTX3 antibody (AB106827)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise