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AB313403

Anti-PITX3/PTX3 antibody [RP23040049]

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(1 Publication)

Rabbit Recombinant Multiclonal PITX3/PTX3 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PITX3.

View Alternative Names

PTX3, PITX3, Pituitary homeobox 3, Homeobox protein PITX3, Paired-like homeodomain transcription factor 3

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PITX3/PTX3 Antibody [RP23040049] (AB313403)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PITX3/PTX3 Antibody [RP23040049] (AB313403)

Immunofluorescent analysis of HeLa cells fixed with 4% formaldehyde reconstituted in 1X PBS for 10 min at room temperature and permeabilized with 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling PITX3/PTX3 using ab313403 followed by Alexa Fluor® 488-conjugated Goat anti-Rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor® 594 phalloidin (red) (image C). Image D is a composite image showing localization of PITX3/PTX3.

Western blot - Anti-PITX3/PTX3 Antibody [RP23040049] (AB313403)
  • WB

Supplier Data

Western blot - Anti-PITX3/PTX3 Antibody [RP23040049] (AB313403)

All lanes:

Western blot - Anti-PITX3/PTX3 Antibody [RP23040049] (ab313403) at 2 µg/mL

All lanes:

Mouse brain lysate

Observed band size: 32 kDa

true

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040049

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human PITX3.

O75364

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-3 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2-5 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PITX3 also known as paired-like homeodomain transcription factor 3 is a transcription factor that plays a role in gene expression regulation. It has a mass of approximately 32 kDa. PITX3 is predominantly found in the eye lens and dopaminergic neurons specifically in the substantia nigra. This region of the brain is vital for motor control. PITX3 expression is important in ocular development and maintaining cellular function in these regions.
Biological function summary

The PITX3 protein regulates critical processes in ocular development and survival of dopaminergic neurons. It is not part of a complex itself but works closely with other regulatory factors influencing various genes involved in neuron differentiation and eye structures. PITX3's activity in these biological systems is fundamental to proper development and cellular homeostasis.

Pathways

PITX3 plays a significant role in the development and function of dopaminergic neurons of the substantia nigra as well as in the formation of eye tissues. It is involved in pathways like Wnt and dopamine synthesis which are critical for neuronal function and vision development. PITX3 interacts with proteins such as Nurr1 in these pathways contributing to neuron survival and differentiation. The balance of these processes directly impacts the development and function of target tissues.

PITX3 mutations are closely associated with ocular diseases like congenital cataracts and neurodegenerative disorders such as Parkinson's disease. In the context of Parkinson's disease PITX3's role alongside proteins like alpha-synuclein is critical; dysregulation in their interaction can lead to dopaminergic neuron degeneration. Understanding PITX3 interactions with other proteins helps illuminate the molecular basis of these conditions offering potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator which is important for the differentiation and maintenance of meso-diencephalic dopaminergic (mdDA) neurons during development. In addition to its importance during development, it also has roles in the long-term survival and maintenance of the mdDA neurons. Activates NR4A2/NURR1-mediated transcription of genes such as SLC6A3, SLC18A2, TH and DRD2 which are essential for development of mdDA neurons. Acts by decreasing the interaction of NR4A2/NURR1 with the corepressor NCOR2/SMRT which acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state. Essential for the normal lens development and differentiation. Plays a critical role in the maintenance of mitotic activity of lens epithelial cells, fiber cell differentiation and in the control of the temporal and spatial activation of fiber cell-specific crystallins. Positively regulates FOXE3 expression and negatively regulates PROX1 in the anterior lens epithelium, preventing activation of CDKN1B/P27Kip1 and CDKN1C/P57Kip2 and thus maintains lens epithelial cells in cell cycle (By similarity).
See full target information PITX3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:108670 PubMed38155767

2023

Inhibition of 7α,26-dihydroxycholesterol biosynthesis promotes midbrain dopaminergic neuron development.

Applications

Unspecified application

Species

Unspecified reactive species

James Hennegan,Aled H Bryant,Lauren Griffiths,Matthieu Trigano,Oliver J M Bartley,Joanna J Bartlett,Carys Minahan,Willy Antoni Abreu de Oliveira,Eylan Yutuc,Sotirios Ntikas,Christos S Bartsocas,Margarita Markouri,Eleni Antoniadou,Ioanna Laina,Owain W Howell,Meng Li,Yuqin Wang,William J Griffiths,Emma L Lane,Mariah J Lelos,Spyridon Theofilopoulos
View all publications
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