AB12337

Anti-PIWIL1 antibody

Name: Anti-PIWIL1 antibody (ab12337) | Abcam
Brand: Abcam Limited
SKU: AB12337
Price: 415 USD
Availability: InStock
Rating: 5 (4 reviews)

(4 Reviews)

(46 Publications)

Rabbit Polyclonal PIWIL1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 46 publications.

View Alternative Names

HIWI, PIWIL1, Piwi-like protein 1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-PIWIL1 antibody (AB12337)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PIWIL1 antibody (AB12337)

ICC/IF image of ab12337 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab12337 at 1μg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Project

Western blot - Anti-PIWIL1 antibody (AB12337)

ab12337 gave a positive signal in both mouse and rat testis lysates (positive controls). Bands were also observed in mouse and rat liver lysates (negative controls) that were higher in size to that observed in testis.

All lanes:

Western blot - Anti-PIWIL1 antibody (ab12337) at 1 µg/mL

Lane 1:

Testis (Mouse) Tissue Lysate at 10 µg

Lane 2:

Liver (Mouse) Tissue Lysate at 10 µg

Lane 3:

Testis (Rat) Tissue Lysate - normal tissue (<a href='/en-us/products/unavailable/testis-rat-tissue-lysate-normal-tissue-ab29388'>ab29388</a>) at 10 µg

Lane 4:

Liver (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 98 kDa

Observed band size: 102 kDa,99 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "ab12337 detects a 99 kDa band in both mouse and rat testis lysates (positive controls). Non-specific bands at 102 kDa were also observed in mouse and rat liver lysates (negative controls).", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "ab12337 detects a 99 kDa band in both mouse and rat testis lysates (positive controls). Non-specific bands at 102 kDa were also observed in mouse and rat liver lysates (negative controls).", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PIWIL1 also known as Piwi-like protein 1 is a member of the Argonaute family with a molecular mass of approximately 97 kDa. This protein functions mechanically by binding to PIWI-interacting RNAs (piRNAs) which are small non-coding RNAs. PIWIL1 exhibits expression in germline cells specifically in testes therefore playing an important role in spermatogenesis. It facilitates the formation of piRNA complexes contributing to the regulation of gene silencing.

Biological function summary

PIWIL1 contributes to maintaining genomic integrity through its role in RNA-mediated gene regulation. It forms part of the piRNA-induced silencing complex. This complex targets transposable elements and prevents their mobility an important function for genomic stability. PIWIL1's involvement in gametogenesis highlights its essential function in reproductive biology and cellular processes where piRNA pathways operate.

Pathways

The PIWIL1 protein plays significant roles within the piRNA pathway which is important for transposon silencing and germline integrity. PIWIL1 interacts with MILI and MIWI two proteins that also participate in the silencing of transposable elements. This pathway intersects with other gene regulation pathways influencing the overall expression landscape necessary for proper cellular function in reproductive tissues.

PIWIL1 alterations have associations with germ cell tumors and fertility issues. Disruption in piRNA pathway components like PIWIL1 and its partners MIWI and MILI can lead to failures in genomic defense mechanisms influencing cancer development in germline cells. Additionally defects in PIWIL1 expression can contribute to infertility due to its role in spermatogenesis. Understanding these associations provides insights into potential diagnostic and therapeutic targets for related conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Endoribonuclease that plays a central role in postnatal germ cells by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Strongly prefers a uridine in the first position of their guide (g1U preference, also named 1U-bias). Not involved in the piRNA amplification loop, also named ping-pong amplification cycle. Acts as an endoribonuclease that cleaves transposon messenger RNAs. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability. Required to sequester RNF8 in the cytoplasm until late spermatogenesis; RNF8 being released upon ubiquitination and degradation of PIWIL1.. Isoform 3. May be a negative developmental regulator (PubMed : 12037681, PubMed : 16287078).

See full target information PIWIL1

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Publications (46)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:15732 PubMed38977826

2024

The dynamic expression of YAP is essential for the development of male germ cells derived from human embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sujittra Khampang,Chanchao Lorthongpanich,Chuti Laowtammathron,Phatchanat Klaihmon,Sukanya Meesa,Wichuda Suksomboon,Nittaya Jiamvoraphong,Pakpoom Kheolamai,Sudjit Luanpitpong,Charles A Easley,Eisa Mahyari,Surapol Issaragrisil

International journal of molecular sciences 24: PubMed37298298

2023

cDNA Cloning of Feline PIWIL1 and Evaluation of Expression in the Testis of the Domestic Cat.

Applications

Unspecified application

Species

Unspecified reactive species

Leanne Stalker,Alanna G Backx,Allison K Tscherner,Stewart J Russell,Robert A Foster,Jonathan LaMarre

Frontiers in oncology 11:783583 PubMed35083142

2022

PIWIL1 Drives Chemoresistance in Multiple Myeloma by Modulating Mitophagy and the Myeloma Stem Cell Population.

Applications

Unspecified application

Species

Unspecified reactive species

Yajun Wang,Lan Yao,Yao Teng,Hua Yin,Qiuling Wu

Molecular cell 81:4826-4842.e8 PubMed34626567

2021

Terminal modification, sequence, length, and PIWI-protein identity determine piRNA stability.

Applications

Unspecified application

Species

Unspecified reactive species

Ildar Gainetdinov,Cansu Colpan,Katharine Cecchini,Amena Arif,Karina Jouravleva,Paul Albosta,Joel Vega-Badillo,Yongjin Lee,Deniz M Özata,Phillip D Zamore

Frontiers in endocrinology 12:696121 PubMed34326815

2021

Decreased piRNAs in Infertile Semen Are Related to Downregulation of Sperm MitoPLD Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yeting Hong,Yanqian Wu,Jianbin Zhang,Chong Yu,Lu Shen,Hanxiao Chen,Linjie Chen,Xue Zhou,Fang Gao

BMC biology 19:86 PubMed33910563

2021

Rat PRDM9 shapes recombination landscapes, duration of meiosis, gametogenesis, and age of fertility.

Applications

Unspecified application

Species

Unspecified reactive species

Ondrej Mihola,Vladimir Landa,Florencia Pratto,Kevin Brick,Tatyana Kobets,Fitore Kusari,Srdjan Gasic,Fatima Smagulova,Corinne Grey,Petr Flachs,Vaclav Gergelits,Karel Tresnak,Jan Silhavy,Petr Mlejnek,R Daniel Camerini-Otero,Michal Pravenec,Galina V Petukhova,Zdenek Trachtulec

Cell reports 34:108522 PubMed33406417

2021

Piwil1 Regulates Glioma Stem Cell Maintenance and Glioblastoma Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Haidong Huang,Xingjiang Yu,Xiangzi Han,Jing Hao,Jianjun Zhao,Gurkan Bebek,Shideng Bao,Richard A Prayson,Ahmad M Khalil,Eckhard Jankowsky,Jennifer S Yu

Cancer biology & therapy 21:1025-1032 PubMed33073675

2020

Long non-coding RNA FALEC promotes colorectal cancer progression via regulating miR-2116-3p-targeted PIWIL1.

Applications

Unspecified application

Species

Unspecified reactive species

Huiyuan Jiang,Haiyi Liu,Bo Jiang

Archives of gynecology and obstetrics 302:925-933 PubMed32613450

2020

PIWIL2 is overexpressed in adenomyotic lesions of women with diffuse adenomyosis.

Applications

Unspecified application

Species

Unspecified reactive species

Marília M C Mattia,Alexandra C P Fernandes,Vanessa K Genro,Carlos Augusto B de Souza,Pedro da Rocha Olsen,João Sabino Cunha-Filho

RNA biology 17:1613-1624 PubMed32372724

2020

Role of PIWI-like 4 in modulating neuronal differentiation from human embryonal carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Charannya Sozheesvari Subhramanyam,Qiong Cao,Cheng Wang,Zealyn Shi Lin Heng,Zhihong Zhou,Qidong Hu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PIWIL1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-PIWIL1 antibody (AB12337)

Western blot - Anti-PIWIL1 antibody (AB12337)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (46)

Product promise