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AB308014

Anti-PIWIL1 antibody [RP23040046]

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Rabbit Recombinant Multiclonal PIWIL1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PIWIL1.

View Alternative Names

HIWI, PIWIL1, Piwi-like protein 1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-PIWIL1 antibody [RP23040046] (AB308014)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PIWIL1 antibody [RP23040046] (AB308014)

Immunofluorescence analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized using 70% confluent log phase HeLa cells labelling PIWIL1 with ab308014 at a concentration of 1 µg/mL, followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor® 488, at a 1 : 2000 dilution (green). Nuclei (Panel b : Blue) were stained with DAPI. F-actin (Panel c : Red) was stained with Rhodamine Phalloidin (1 : 300 dilution). Panel d represents the merged image showing nuclear and cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunocytochemistry/ Immunofluorescence - Anti-PIWIL1 antibody [RP23040046] (AB308014)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PIWIL1 antibody [RP23040046] (AB308014)

Immunofluorescent analysis of PIWIL1 in HeLa cells using a PIWIL1 Recombinant Rabbit Polyclonal Antibody (ab308014) followed by detection using an Alexa Fluor® 488-conjugated Goat anti-Rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and actin stained with Alexa Fluor® 594 phalloidin (red) (image C). Image D is a composite image showing cytoplasmic localization of PIWIL1.

Western blot - Anti-PIWIL1 antibody [RP23040046] (AB308014)
  • WB

Supplier Data

Western blot - Anti-PIWIL1 antibody [RP23040046] (AB308014)

Western blot analysis of PIWIL1 in COLO 205 whole cell extracts using ab308014 at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~99kDa.

All lanes:

Western blot - Anti-PIWIL1 antibody [RP23040046] (ab308014) at 2 µg/mL

All lanes:

COLO 205 whole cell lysate

Observed band size: 99 kDa

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Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040046

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human PIWIL1.

Q96J94

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PIWIL1 also known as Piwi-like protein 1 is a member of the Argonaute family with a molecular mass of approximately 97 kDa. This protein functions mechanically by binding to PIWI-interacting RNAs (piRNAs) which are small non-coding RNAs. PIWIL1 exhibits expression in germline cells specifically in testes therefore playing an important role in spermatogenesis. It facilitates the formation of piRNA complexes contributing to the regulation of gene silencing.
Biological function summary

PIWIL1 contributes to maintaining genomic integrity through its role in RNA-mediated gene regulation. It forms part of the piRNA-induced silencing complex. This complex targets transposable elements and prevents their mobility an important function for genomic stability. PIWIL1's involvement in gametogenesis highlights its essential function in reproductive biology and cellular processes where piRNA pathways operate.

Pathways

The PIWIL1 protein plays significant roles within the piRNA pathway which is important for transposon silencing and germline integrity. PIWIL1 interacts with MILI and MIWI two proteins that also participate in the silencing of transposable elements. This pathway intersects with other gene regulation pathways influencing the overall expression landscape necessary for proper cellular function in reproductive tissues.

PIWIL1 alterations have associations with germ cell tumors and fertility issues. Disruption in piRNA pathway components like PIWIL1 and its partners MIWI and MILI can lead to failures in genomic defense mechanisms influencing cancer development in germline cells. Additionally defects in PIWIL1 expression can contribute to infertility due to its role in spermatogenesis. Understanding these associations provides insights into potential diagnostic and therapeutic targets for related conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Endoribonuclease that plays a central role in postnatal germ cells by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Strongly prefers a uridine in the first position of their guide (g1U preference, also named 1U-bias). Not involved in the piRNA amplification loop, also named ping-pong amplification cycle. Acts as an endoribonuclease that cleaves transposon messenger RNAs. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability. Required to sequester RNF8 in the cytoplasm until late spermatogenesis; RNF8 being released upon ubiquitination and degradation of PIWIL1.. Isoform 3. May be a negative developmental regulator (PubMed : 12037681, PubMed : 16287078).
See full target information PIWIL1
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Product promise

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