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Mouse Monoclonal PIWIL2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIWIL2 antibody [87/3] (AB151398), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-PIWIL2 antibody [87/3] (AB151398), expandable thumbnail

Publications

Key facts

Isotype
IgM
Host species
Mouse
Storage buffer
Standard buffer
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IF
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
10 µg/mL
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
10 µg/mL
Notes

-

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Target data

Function

Endoribonuclease that plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity). Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells (By similarity). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons (By similarity). During piRNA biosynthesis, plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle, by acting as a 'slicer-competent' piRNA endoribonuclease that cleaves primary piRNAs, which are then loaded onto 'slicer-incompetent' PIWIL4 (By similarity). PIWIL2 slicing produces a pre-miRNA intermediate, which is then processed in mature piRNAs, and as well as a 16 nucleotide by-product that is degraded (By similarity). Required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense (By similarity). Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). Indirectly modulates expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8 (By similarity). When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors (PubMed:16377660). Represses circadian rhythms by promoting the stability and activity of core clock components BMAL1 and CLOCK by inhibiting GSK3B-mediated phosphorylation and ubiquitination-dependent degradation of these proteins (PubMed:28903391).

Alternative names

HILI, PIWIL2, Piwi-like protein 2, Cancer/testis antigen 80, CT80

Recommended products

Mouse Monoclonal PIWIL2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgM
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
87/3
Concentration
Loading...

Notes

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PIWIL2 also known as piwi-like RNA-mediated gene silencing 2 is a protein with a mass of approximately 98 kDa. This protein belongs to the PIWI subfamily of Argonaute proteins and is expressed across various tissues including testis and several somatic cells. PIWIL2 plays an important role in regulating gene expression through the RNA interference pathway engaging in the formation of piRNA complexes that facilitate the silencing of transposons especially in spermatogenesis.

Biological function summary

PIWIL2 functions to maintain genomic integrity and stem cell self-renewal by interacting with piRNA to suppress the activity of mobile genetic elements. It forms a ribonucleoprotein complex that is necessary for piRNA processing and function. This complex formation is vital in the regulation of transposable elements influencing processes like germ cell development and stem cell maintenance.

Pathways

PIWIL2 participates in both the RNA interference and piRNA pathway impacting gene regulatory networks critical for cell division and differentiation. In the piRNA pathway PIWIL2 associates with other proteins such as MILI and MIWI to ensure correct piRNA maturation and function. The presence of PIWIL2 in these pathways is non-redundant and important for facilitating silencing mechanisms that protect genome stability.

Associated diseases and disorders

Altered PIWIL2 expression or function links to several types of cancer including testicular and breast cancer. PIWIL2’s interaction with other cancer-related proteins like p53 may influence tumorigenesis through the modulation of gene silencing. This association highlights its potential as a biomarker for cancer prognosis and as a target for therapeutic intervention.

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2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIWIL2 antibody [87/3] (ab151398), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIWIL2 antibody [87/3] (ab151398)

    IHC image of PIWIL2 staining in human breast ductal carcinoma formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab151398, 10μg/ml overnight at +4°C. An HRP-conjugated secondary (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040, 1/1000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Immunocytochemistry/ Immunofluorescence - Anti-PIWIL2 antibody [87/3] (ab151398), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PIWIL2 antibody [87/3] (ab151398)

    ab151398 stained MDAMB231 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab151398 at 10μg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab98674) IgM (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

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Product protocols

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