AB85084

Anti-PIWIL2 antibody

Name: Anti-PIWIL2 antibody (ab85084) | Abcam
Brand: Abcam Limited
SKU: AB85084
Price: 535 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(7 Publications)

Rabbit Polyclonal PIWIL2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human PIWIL2 aa 400-450.

View Alternative Names

HILI, PIWIL2, Piwi-like protein 2, Cancer/testis antigen 80, CT80

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIWIL2 antibody (AB85084)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIWIL2 antibody (AB85084)

Immunohistochemistry analysis of PIWIL2 in formalin-fixed paraffin-embedded human fetal testis tissue sections using ab85084 at 1/200 dilution.

Unknown

Western blot - Anti-PIWIL2 antibody (AB85084)

All lanes:

Western blot - Anti-PIWIL2 antibody (ab85084) at 1/200 dilution

All lanes:

Human fetal testis lysate

Predicted band size: 110 kDa

Observed band size: 110 kDa,55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human PIWIL2 aa 400-450. The exact immunogen used to generate this antibody is proprietary information.

Q8TC59

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/500", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/400", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Lyophilized

Reconstitution

reconstitute with water at 200µL

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: 1% BSA, 0.184% Tris glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PIWIL2 also known as piwi-like RNA-mediated gene silencing 2 is a protein with a mass of approximately 98 kDa. This protein belongs to the PIWI subfamily of Argonaute proteins and is expressed across various tissues including testis and several somatic cells. PIWIL2 plays an important role in regulating gene expression through the RNA interference pathway engaging in the formation of piRNA complexes that facilitate the silencing of transposons especially in spermatogenesis.

Biological function summary

PIWIL2 functions to maintain genomic integrity and stem cell self-renewal by interacting with piRNA to suppress the activity of mobile genetic elements. It forms a ribonucleoprotein complex that is necessary for piRNA processing and function. This complex formation is vital in the regulation of transposable elements influencing processes like germ cell development and stem cell maintenance.

Pathways

PIWIL2 participates in both the RNA interference and piRNA pathway impacting gene regulatory networks critical for cell division and differentiation. In the piRNA pathway PIWIL2 associates with other proteins such as MILI and MIWI to ensure correct piRNA maturation and function. The presence of PIWIL2 in these pathways is non-redundant and important for facilitating silencing mechanisms that protect genome stability.

Altered PIWIL2 expression or function links to several types of cancer including testicular and breast cancer. PIWIL2’s interaction with other cancer-related proteins like p53 may influence tumorigenesis through the modulation of gene silencing. This association highlights its potential as a biomarker for cancer prognosis and as a target for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Endoribonuclease that plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity). Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells (By similarity). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons (By similarity). During piRNA biosynthesis, plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle, by acting as a 'slicer-competent' piRNA endoribonuclease that cleaves primary piRNAs, which are then loaded onto 'slicer-incompetent' PIWIL4 (By similarity). PIWIL2 slicing produces a pre-miRNA intermediate, which is then processed in mature piRNAs, and as well as a 16 nucleotide by-product that is degraded (By similarity). Required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense (By similarity). Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). Indirectly modulates expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8 (By similarity). When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors (PubMed : 16377660). Represses circadian rhythms by promoting the stability and activity of core clock components BMAL1 and CLOCK by inhibiting GSK3B-mediated phosphorylation and ubiquitination-dependent degradation of these proteins (PubMed : 28903391).

See full target information PIWIL2

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Animals : an open access journal from MDPI 13: PubMed36670859

2023

Comparative Analysis of the Potential for Germ Cell (GC) Differentiation of Bovine Peripheral Blood Derived-Mesenchymal Stem Cells (PB-MSC) and Spermatogonial Stem Cells (SSC) in Co-Culture System with Sertoli Cells (SC).

Applications

Unspecified application

Species

Unspecified reactive species

Moisés N Segunda,Carlos Díaz,Cristian G Torres,Víctor H Parraguez,Mónica De Los Reyes,Oscar A Peralta

Scientific reports 11:19593 PubMed34599264

2021

Interferon-inducible protein, IFIX, has tumor-suppressive effects in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Wang,Fang Li,Haixia Fan

Neoplasia (New York, N.Y.) 23:1-11 PubMed33190089

2020

CBP-mediated Wnt3a/β-catenin signaling promotes cervical oncogenesis initiated by Piwil2.

Applications

Unspecified application

Species

Unspecified reactive species

Dingqing Feng,Keqin Yan,Haiyan Liang,Jing Liang,Wenhui Wang,Huan Yu,Ying Zhou,Weidong Zhao,Zhongjun Dong,Bin Ling

Journal of cellular and molecular medicine 24:5260-5273 PubMed32227582

2020

piRNA-63076 contributes to pulmonary arterial smooth muscle cell proliferation through acyl-CoA dehydrogenase.

Applications

Unspecified application

Species

Unspecified reactive species

Cui Ma,Lixin Zhang,Xiaoying Wang,Siyu He,June Bai,Qian Li,Min Zhang,Chen Zhang,Xiufeng Yu,Junting Zhang,Wei Xin,Yiying Li,Daling Zhu

Cancer biomarkers : section A of Disease markers 26:11-20 PubMed31322538

2019

Expression of PIWIL2 in oral cancer and leukoplakia: Prognostic implications and insights from tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Wang,Fang Li,Haixia Fan,Jiankai Xu,Zheng Hu

Oncotarget 7:64575-64588 PubMed27602489

2016

Piwil2 is reactivated by HPV oncoproteins and initiates cell reprogramming via epigenetic regulation during cervical cancer tumorigenesis.

Applications

WB, IHC

Species

Human, Human

Dingqing Feng,Keqin Yan,Ying Zhou,Haiyan Liang,Jing Liang,Weidong Zhao,Zhongjun Dong,Bin Ling

The American journal of pathology 181:2079-93 PubMed23031258

2012

DNA methylation plasticity of human adipose-derived stem cells in lineage commitment.

Applications

Species

Unspecified reactive species

María Berdasco,Consolación Melguizo,Jose Prados,Antonio Gómez,Miguel Alaminos,Miguel A Pujana,Miguel Lopez,Fernando Setien,Raul Ortiz,Inma Zafra,Antonia Aranega,Manel Esteller

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-PIWIL2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIWIL2 antibody (AB85084)

Western blot - Anti-PIWIL2 antibody (AB85084)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Reconstitution

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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