Rabbit Polyclonal PIWIL4/PIWI antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PIWIL4 aa 600-850.
Images
Publications
Filter by
- Journal of translational medicine 20:512022piRNA-14633 promotes cervical cancer cell malignancy in a METTL14-dependent m6A RNA methylation manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Xie,Zhen Li,Xiao Luo,Dan Wang,Yao Zhou,Jingge Zhao,Suhua Gao,Yongguang Yang,Wanying Fu,Lingfei Kong,Tingyi SunPubMed 35093098
- Molecular neurobiology 59:1693-17052022piRNA/PIWI Protein Complex as a Potential Biomarker in Sporadic Amyotrophic Lateral Sclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRehab F Abdelhamid,Kotaro Ogawa,Goichi Beck,Kensuke Ikenaka,Eriko Takeuchi,Yoshiaki Yasumizu,Jyunki Jinno,Yasuyoshi Kimura,Kousuke Baba,Yoshitaka Nagai,Yukinori Okada,Yuko Saito,Shigeo Murayama,Hideki Mochizuki,Seiichi NaganoPubMed 35015250
- Aging 13:23361-233752021piRNA-36741 regulates BMP2-mediated osteoblast differentiation via METTL3 controlled m6A modification.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianmin Liu,Ming Chen,Longyang Ma,Xingbo Dang,Gongliang DuPubMed 34645714
- Oncotarget 9:37616-376262018piRNA-8041 is downregulated in human glioblastoma and suppresses tumor growth and .Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel I Jacobs et. al.PubMed 30701019
- Scientific reports 8:164312018PIWI proteins contribute to apoptosis during the UPR in human airway epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMagdalena Gebert et. al.PubMed 30401887
- Genes, chromosomes & cancer 57:339-3492018Investigating piwi-interacting RNA regulome in human neuroblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJyoti Roy,Bibekanand MallickPubMed 29516567
- PloS one 13:e01904852018Genome-wide profiling of the PIWI-interacting RNA-mRNA regulatory networks in epithelial ovarian cancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGarima Singh et. al.PubMed 29320577
- Oncotarget 7:46002-460132016PiwiRNA-651 as marker of treatment response and survival in classical Hodgkin lymphoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Cordeiro,Alfons Navarro,Anna Gaya,Marina Díaz-Beyá,Blanca Gonzalez-Farré,Joan Josep Castellano,Dolors Fuster,Carmen Martínez,Antonio Martínez,Mariano MonzóPubMed 27329591
- The Journal of biological chemistry 291:10646-582016The Role of PIWIL4, an Argonaute Family Protein, in Breast Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZifeng Wang et. al.PubMed 26957540
- Oncotarget 5:9901-102014RNA sequencing identifies specific PIWI-interacting small non-coding RNA expression patterns in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdnan Hashim,Francesca Rizzo,Giovanna Marchese,Maria Ravo,Roberta Tarallo,Giovanni Nassa,Giorgio Giurato,Gianluca Santamaria,Angela Cordella,Concita Cantarella,Alessandro WeiszPubMed 25313140
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human PIWIL4 aa 600-850. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z3Z4
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Monkey | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes or Tris-EDTA buffer ( PH8.0) |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (By similarity). Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements (By similarity). Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association (By similarity). The piRNA process acts upstream of known mediators of DNA methylation (By similarity). Does not show endonuclease activity (By similarity). Plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle, by acting as a 'slicer-incompetent' component that loads cleaved piRNAs from the 'slicer-competent' component PIWIL2 and target them on genomic transposon loci in the nucleus (By similarity). May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci (PubMed:17544373). In addition to its role in germline, PIWIL4 also plays a role in the regulation of somatic cells activities. Plays a role in pancreatic beta cell function and insulin secretion (By similarity). Involved in maintaining cell morphology and functional integrity of retinal epithelial through Akt/GSK3alpha/beta signaling pathway (PubMed:28025795). When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of cells proliferation in tumors (PubMed:22483988).
Alternative names
HIWI2, PIWI, PIWIL4, Piwi-like protein 4
Recommended products
Rabbit Polyclonal PIWIL4/PIWI antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PIWIL4 aa 600-850.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human PIWIL4 aa 600-850. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z3Z4
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab111714 is purified by antigen-affinity chromatography.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PIWIL4 also known as PIWI-like 4 is a protein involved in RNA-mediated gene silencing with a molecular weight of approximately 97 kDa. It is expressed mostly in germ cell tissues including the testes and ovaries. PIWIL4 belongs to the PIWI subfamily of Argonaute proteins which is critical for the regulation of gene expression at the transcriptional and post-transcriptional levels.
- Biological function summary
PIWIL4 plays an important role in the process of germ cell development and maintenance. It is essential for the formation of piRNA clusters and interacts with small non-coding RNA known as PIWI-interacting RNA (piRNA). It functions as part of a protein complex that facilitates the silencing of transposable elements and the regulation of gene expression therefore safeguarding genome integrity in germ cells.
- Pathways
PIWIL4 integrates within the piRNA biogenesis pathway and RNA interference (RNAi) pathway key processes in cellular defense mechanisms. It associates with other PIWI family members such as PIWIL1 and PIWIL2 which collaborate to cleave and silence transposable elements and undesirable genetic elements. This function is critical in pausing transposition events that can disrupt genomic stability.
- Associated diseases and disorders
PIWIL4 has connections to certain cancers such as testicular germ cell tumor due to its role in regulating cell proliferation and genome protection. Abnormal expression or malfunction of PIWIL4 has implications in the development of infertility as it influences germ cell maturation. Investigations also assess its interactions with proteins like MAEL and TDRD1 which contribute to the maintenance of spermatogenic functions in testicular tissues.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-PIWIL4/PIWI antibody (ab111714)
7.5% SDS-PAGE.
All lanes: Western blot - Anti-PIWIL4/PIWI antibody (ab111714) at 1/1000 dilution
All lanes: MCF7 whole cell lysate at 30 µg
Predicted band size: 97 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PIWIL4/PIWI antibody (ab111714)
ab111714 at 1/200 dilution staining PIWIL4/PIWI in Paraformaldehyde-fixed MCF7 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PIWIL4/PIWI antibody (ab111714)
ab111714 at 1/500 dilution staining PIWIL4/PIWI in Formalin-fixed, paraffin-embedded 59T xenograft tissue by Immunohistochemistry.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com