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Rabbit Recombinant Monoclonal PIWIL4/PIWI antibody. Suitable for IP, WB and reacts with Human, Mouse samples.

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Images

Western blot - Anti-PIWIL4/PIWI antibody [EPR28186-10] (AB324050), expandable thumbnail
  • Western blot - Anti-PIWIL4/PIWI antibody [EPR28186-10] (AB324050), expandable thumbnail
  • Western blot - Anti-PIWIL4/PIWI antibody [EPR28186-10] (AB324050), expandable thumbnail
  • Western blot - Anti-PIWIL4/PIWI antibody [EPR28186-10] (AB324050), expandable thumbnail
  • Immunoprecipitation - Anti-PIWIL4/PIWI antibody [EPR28186-10] (AB324050), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Mouse
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Species
Mouse
Dilution info
1/30
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Target data

Function

Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity). Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (By similarity). Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements (By similarity). Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association (By similarity). The piRNA process acts upstream of known mediators of DNA methylation (By similarity). Does not show endonuclease activity (By similarity). Plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle, by acting as a 'slicer-incompetent' component that loads cleaved piRNAs from the 'slicer-competent' component PIWIL2 and target them on genomic transposon loci in the nucleus (By similarity). May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci (PubMed:17544373). In addition to its role in germline, PIWIL4 also plays a role in the regulation of somatic cells activities. Plays a role in pancreatic beta cell function and insulin secretion (By similarity). Involved in maintaining cell morphology and functional integrity of retinal epithelial through Akt/GSK3alpha/beta signaling pathway (PubMed:28025795). When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of cells proliferation in tumors (PubMed:22483988).

Additional Targets

Piwil4

Alternative names

HIWI2, PIWI, PIWIL4, Piwi-like protein 4

Rabbit Recombinant Monoclonal PIWIL4/PIWI antibody. Suitable for IP, WB and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28186-10
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-PIWIL4/PIWI antibody [EPR28186-10] (ab324050), expandable thumbnail

    Western blot - Anti-PIWIL4/PIWI antibody [EPR28186-10] (ab324050)

    PIWIL4/PIWI Western blot staining using rabbit Anti-PIWIL4/PIWI antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: skeletal muscle, liver, ovary (PMID: 24108303).

    To minimize protein degradation, tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-PIWIL4/PIWI antibody [EPR28186-10] (ab324050) at 1/1000 dilution

    Lane 1: Mouse P1 testis tissue lysate at 50 µg

    Lane 2: Mouse P1 skeletal muscle tissue lysate at 50 µg

    Lane 3: Mouse P1 liver tissue lysate at 50 µg

    Lane 4: Mouse P1 ovary tissue lysate at 50 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 96 kDa, 124 kDa

    Exposure time: 26s

  • Western blot - Anti-PIWIL4/PIWI antibody [EPR28186-10] (ab324050), expandable thumbnail

    Western blot - Anti-PIWIL4/PIWI antibody [EPR28186-10] (ab324050)

    PIWIL4/PIWI Western blot staining using rabbit Anti-PIWIL4/PIWI antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: P60 testis (PMID:25038252).

    To minimize protein degradation, tissues were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.

    All lanes: Western blot - Anti-PIWIL4/PIWI antibody [EPR28186-10] (ab324050) at 1/1000 dilution

    Lane 1: Mouse P1 testis tissue lysate at 50 µg

    Lane 2: Mouse P60 testis tissue lysate at 50 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 96 kDa, 124 kDa

    Exposure time: 48s

  • Western blot - Anti-PIWIL4/PIWI antibody [EPR28186-10] (ab324050), expandable thumbnail

    Western blot - Anti-PIWIL4/PIWI antibody [EPR28186-10] (ab324050)

    PIWIL4/PIWI Western blot staining of Human testis tissue lysate using rabbit Anti-PIWIL4/PIWI antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PIWIL4/PIWI antibody [EPR28186-10] (ab324050) at 1/1000 dilution

    All lanes: Human testis tissue lysate at 60 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 96 kDa

    Exposure time: 26s

  • Western blot - Anti-PIWIL4/PIWI antibody [EPR28186-10] (ab324050), expandable thumbnail

    Western blot - Anti-PIWIL4/PIWI antibody [EPR28186-10] (ab324050)

    PIWIL4/PIWI Western blot staining using rabbit Anti-PIWIL4/PIWI antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-PIWIL4/PIWI antibody [EPR28186-10] (ab324050) at 1/1000 dilution

    Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) transfected with scrambled siRNA control whole cell lysate at 60 µg

    Lane 2: K-562 transfected with siRNA specifically targeting PIWIL4/PIWI whole cell lysate at 60 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 96 kDa, 36 kDa

    Exposure time: 180s

  • Immunoprecipitation - Anti-PIWIL4/PIWI antibody [EPR28186-10] (ab324050), expandable thumbnail

    Immunoprecipitation - Anti-PIWIL4/PIWI antibody [EPR28186-10] (ab324050)

    PIWIL4/PIWI was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab324050 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab324050 at 1/1000 dilution.

    Blocking and dilution buffer and concentration: 5%

    To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

    All lanes: Immunoprecipitation - Anti-PIWIL4/PIWI antibody [EPR28186-10] (ab324050) at 1/1000 dilution

    Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 10 µg

    Lane 2: ab324050 at 1/30 IP in K-562 whole cell lysate at 10 µg

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab324050 in K-562 whole cell lysate at 10 µg

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 96 kDa

    Exposure time: 111s

  • Immunoprecipitation - Anti-PIWIL4/PIWI antibody [EPR28186-10] (ab324050), expandable thumbnail

    Immunoprecipitation - Anti-PIWIL4/PIWI antibody [EPR28186-10] (ab324050)

    PIWIL4/PIWI was immunoprecipitated from 0.35 mg Mouse P7 testis tissue lysate with ab324050 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab324050 at 1/1000 dilution.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-PIWIL4/PIWI antibody [EPR28186-10] (ab324050) at 1/1000 dilution

    Lane 1: Mouse P7 testis tissue lysate at 10 µg

    Lane 2: ab324050 at 1/30 IP in mouse P7 testis tissue lysate at 10 µg

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab324050 in mouse P7 testis tissue lysate at 10 µg

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 96 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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