AB216572

Anti-PKA alpha + beta (catalytic subunits) antibody

Name: Anti-PKA alpha + beta (catalytic subunits) antibody (ab216572) | Abcam
Brand: Abcam Limited
SKU: AB216572
Price: 510 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal PKA beta (catalytic subunit) antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Cow PRKACB aa 1-100 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

cAMP-dependent protein kinase catalytic subunit beta, PKA C-beta, PRKACB

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA alpha + beta (catalytic subunits) antibody (AB216572)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA alpha + beta (catalytic subunits) antibody (AB216572)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue labeling cAMP Protein Kinase Catalytic subunit with ab216572 at 1/200 dilution, followed by secondary antibody detection and DAB staining.

Lab

Western blot - Anti-PKA alpha + beta (catalytic subunits) antibody (AB216572)

All lanes:

Western blot - Anti-PKA alpha + beta (catalytic subunits) antibody (ab216572) at 1/500 dilution

All lanes:

Jurkat lysates

Secondary

All lanes:

Conjugated secondary antibody at 1/10000 dilution

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Cow PRKACB aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P05131

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein Kinase A (PKA) alpha and beta catalytic subunits are essential components in cellular signaling. PKA also known as cAMP-dependent protein kinase has its catalytic subunits encoded by PRKACA (alpha) and PRKACB (beta) genes. Alpha subunit has a mass of approximately 40-45 kDa. These proteins are expressed in various tissues including the brain heart and skeletal muscle. They play an important role in phosphorylating target proteins in response to increased cellular levels of cyclic adenosine monophosphate (cAMP).

Biological function summary

PKA alpha and beta catalytic subunits participate in many cellular processes. They are part of a larger complex with regulatory subunits that inhibit their activity. The complex remains inactive until cAMP binds to the regulatory subunits causing a conformational change that activates the catalytic subunits. Once activated PKA subunits phosphorylate various substrates to modulate functions such as metabolism transcription and memory.

Pathways

PKA catalytic subunits serve as key mediators in the cAMP signaling pathway. This pathway influences cellular responses to hormones like adrenaline and glucagon. As such PKA often interacts with proteins such as CREB (cAMP response element-binding protein) to regulate gene expression. PKA also has a role in the regulation of glycogen sugar and lipid metabolism working in concert with proteins like phosphorylase kinase.

Aberrant regulation of PKA catalytic subunits can lead to various conditions. Overactive or dysregulated PKA is associated with Cushing's syndrome often due to mutations in PRKACA. Additionally these catalytic subunits are implicated in certain cancers through alterations in the cAMP signaling pathway. The interaction between PKA and other proteins such as RII-beta (a regulatory subunit) is critical in understanding the molecular basis of these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates GPKOW which regulates its ability to bind RNA. Acts as a negative regulator of mTORC1 by mediating phosphorylation of RPTOR.

See full target information PRKACB

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:4497 PubMed38402335

2024

High-voltage pulsed radiofrequency improves ultrastructure of DRG and enhances spinal microglial autophagy to ameliorate neuropathic pain induced by SNI.

Applications

Unspecified application

Species

Unspecified reactive species

Ri Chen,Xueru Xu,Youfen Yu,Yanqin Chen,Chun Lin,Rongguo Liu

Journal of orthopaedic surgery and research 18:849 PubMed37941009

2023

ITGB1 alleviates osteoarthritis by inhibiting cartilage inflammation and apoptosis via activating cAMP pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lifeng Xie,Zhengnan Li,Zhijun Chen,Mingzhang Li,Jun Tao

Frontiers in pharmacology 14:1081030 PubMed36814484

2023

Cryptochrome 1 activation inhibits melanogenesis and melanosome transport through negative regulation of cAMP/PKA/CREB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Rongyin Gao,Ximei Zhang,Kun Zou,Duo Meng,Jinpeng Lv

Oxidative medicine and cellular longevity 2022:4455654 PubMed36620084

2022

BYHW Decoction Improves Cognitive Impairments in Rats with Cerebral Microinfarcts via Activation of the PKA/CREB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bingjie Xue,Bo Ma,Yaoyao Yao,Aimei Zhao,Ying Gao,Jianxun Liu

Nature communications 12:3352 PubMed34099640

2021

The C5a/C5a receptor 1 axis controls tissue neovascularization through CXCL4 release from platelets.

Applications

Unspecified application

Species

Unspecified reactive species

Henry Nording,Lasse Baron,David Haberthür,Frederic Emschermann,Matthias Mezger,Manuela Sauter,Reinhard Sauter,Johannes Patzelt,Kai Knoepp,Anne Nording,Moritz Meusel,Roza Meyer-Saraei,Ruslan Hlushchuk,Daniel Sedding,Oliver Borst,Ingo Eitel,Christian M Karsten,Robert Feil,Bernd Pichler,Jeanette Erdmann,Admar Verschoor,Emmanouil Chavakis,Triantafyllos Chavakis,Philipp von Hundelshausen,Jörg Köhl,Meinrad Gawaz,Harald F Langer

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PKA alpha + beta (catalytic subunits) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA alpha + beta (catalytic subunits) antibody (AB216572)

Western blot - Anti-PKA alpha + beta (catalytic subunits) antibody (AB216572)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Complementary Products

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Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise