Rabbit Polyclonal PKA beta (catalytic subunit) antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Cow PRKACB aa 1-100 conjugated to Keyhole Limpet Haemocyanin.
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Publications
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- Journal of orthopaedic surgery and research 18:8492023ITGB1 alleviates osteoarthritis by inhibiting cartilage inflammation and apoptosis via activating cAMP pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLifeng Xie,Zhengnan Li,Zhijun Chen,Mingzhang Li,Jun TaoPubMed 37941009
- Frontiers in pharmacology 14:10810302023Cryptochrome 1 activation inhibits melanogenesis and melanosome transport through negative regulation of cAMP/PKA/CREB signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRongyin Gao,Ximei Zhang,Kun Zou,Duo Meng,Jinpeng LvPubMed 36814484
- Oxidative medicine and cellular longevity 2022:44556542022BYHW Decoction Improves Cognitive Impairments in Rats with Cerebral Microinfarcts via Activation of the PKA/CREB Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBingjie Xue,Bo Ma,Yaoyao Yao,Aimei Zhao,Ying Gao,Jianxun LiuPubMed 36620084
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Cow PRKACB aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P05131
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Associated Products
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Target data
Function
Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates GPKOW which regulates its ability to bind RNA. Acts as a negative regulator of mTORC1 by mediating phosphorylation of RPTOR.
Alternative names
cAMP-dependent protein kinase catalytic subunit beta, PKA C-beta, PRKACB
Recommended products
Rabbit Polyclonal PKA beta (catalytic subunit) antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Cow PRKACB aa 1-100 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Cow PRKACB aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P05131
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Protein Kinase A (PKA) alpha and beta catalytic subunits are essential components in cellular signaling. PKA also known as cAMP-dependent protein kinase has its catalytic subunits encoded by PRKACA (alpha) and PRKACB (beta) genes. Alpha subunit has a mass of approximately 40-45 kDa. These proteins are expressed in various tissues including the brain heart and skeletal muscle. They play an important role in phosphorylating target proteins in response to increased cellular levels of cyclic adenosine monophosphate (cAMP).
- Biological function summary
PKA alpha and beta catalytic subunits participate in many cellular processes. They are part of a larger complex with regulatory subunits that inhibit their activity. The complex remains inactive until cAMP binds to the regulatory subunits causing a conformational change that activates the catalytic subunits. Once activated PKA subunits phosphorylate various substrates to modulate functions such as metabolism transcription and memory.
- Pathways
PKA catalytic subunits serve as key mediators in the cAMP signaling pathway. This pathway influences cellular responses to hormones like adrenaline and glucagon. As such PKA often interacts with proteins such as CREB (cAMP response element-binding protein) to regulate gene expression. PKA also has a role in the regulation of glycogen sugar and lipid metabolism working in concert with proteins like phosphorylase kinase.
- Associated diseases and disorders
Aberrant regulation of PKA catalytic subunits can lead to various conditions. Overactive or dysregulated PKA is associated with Cushing's syndrome often due to mutations in PRKACA. Additionally these catalytic subunits are implicated in certain cancers through alterations in the cAMP signaling pathway. The interaction between PKA and other proteins such as RII-beta (a regulatory subunit) is critical in understanding the molecular basis of these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-PKA alpha + beta (catalytic subunits) antibody (ab216572)
All lanes: Western blot - Anti-PKA alpha + beta (catalytic subunits) antibody (ab216572) at 1/500 dilution
All lanes: Jurkat lysates
SecondaryAll lanes: Conjugated secondary antibody at 1/10000 dilution
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKA alpha + beta (catalytic subunits) antibody (ab216572)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue labeling cAMP Protein Kinase Catalytic subunit with ab216572 at 1/200 dilution, followed by secondary antibody detection and DAB staining.
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